Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.7.1.78 extracted from

  • Novogrodsky, A.; Tal, M.; Traub, A.; Hurwitz, J.
    The enzymatic phosphorylation of ribonucleic acid and deoxyribonucleic acid. II. Further properties of the 5-hydroxyl polynucleotide kinase (1966), J. Biol. Chem., 241, 2933-2943.
    View publication on PubMed
Search for more literature for 2.7.1.78

General Stability

General Stability Organism
greatest protection from heat inactivation with substrates for the kinase, e.g. thymus DNA and ribosomal RNA, both micrococcal nuclease-treated and 3'-AMP Escherichia phage T2

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ inhibits in combination with MgCl2, stimulates without MgCl2 Escherichia phage T2
Cu2+ inhibits in presence of CuCl2 Escherichia phage T2
diphosphate
-
 Escherichia phage T2
Maleate
-
 Escherichia phage T2
Ni2+ inhibits in combination with MgCl2, stimulates without MgCl2 Escherichia phage T2
phosphate
-
 Escherichia phage T2

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.1
-
 Mn2+ pH 6.0, 37°C Escherichia phage T2
0.5
-
 Mg2+ pH 6.0, 37°C Escherichia phage T2

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ divalent cation required, slight activation in absence of MgCl2 Escherichia phage T2
Ca2+ inhibition in combination with MgCl2 Escherichia phage T2
Co2+ Mg2+, Mn2+, Zn2+ and Co2+ are equally effective in activation Escherichia phage T2
divalent cations absolutely required Escherichia phage T2
divalent cations Mg2+, Mn2+, Zn2+, Co2+ are equally effective Escherichia phage T2
Mg2+ Mg2+, Mn2+, Zn2+ and Co2+ are equally effective Escherichia phage T2
Mn2+ Mg2+, Mn2+, Zn2+ and Co2+ are equally effective Escherichia phage T2
Ni2+ slight activation in absence of MgCl2, inhibition in combination with MgCl2 Escherichia phage T2
Zn2+ Mg2+, Mn2+, Zn2+ and Co2+ are equally effective in activation Escherichia phage T2

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + 5'-dephospho-DNA Escherichia phage T2
-
 ADP + 5'-phospho-DNA
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia phage T2
-
 from infected Escherichia coli
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 2'(3')-ribonucleotides no activity with (2')-AMP Escherichia phage T2 ?
-
 ?
ATP + 5'-dephospho-DNA
-
 Escherichia phage T2 ADP + 5'-phospho-DNA
-
 ?
ATP + 5'-dephospho-RNA
-
 Escherichia phage T2 ADP + 5'-phospho-RNA
-
 ?
ATP + nucleoside-3'-monophosphate
-
 Escherichia phage T2 ADP + nucleoside-3',5'-diphosphate
-
 ?
CTP + 5'-dephospho-DNA
-
 Escherichia phage T2 CDP + 5'-phospho-DNA
-
 ?
GTP + 5'-dephospho-DNA
-
 Escherichia phage T2 GDP + 5'-phospho-DNA
-
 ?
additional information substrate specificity Escherichia phage T2 ?
-
 ?
additional information no activity with adenosine, nucleotides, and 5'-mononucleotides Escherichia phage T2 ?
-
 ?
UTP + 5'-dephospho-DNA
-
 Escherichia phage T2 UDP + 5'-phospho-DNA
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Escherichia phage T2

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
 protection against heat inactivation by substrates, e.g. calf thymus DNA and ribosomal RNA, both micrococcal nuclease-treated, and 3'-AMP Escherichia phage T2
37
-
 activity is completely lost without substrates Escherichia phage T2

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6 9 broad optimum Escherichia phage T2

pH Range

pH Minimum pH Maximum Comment Organism
5 9.5 about 50% of activity maximum at pH 5.0 and pH 9.5 Escherichia phage T2

Cofactor

Cofactor Comment Organism Structure
ATP dependent on Escherichia phage T2
CDP
-
 Escherichia phage T2
CTP
-
 Escherichia phage T2
GDP
-
 Escherichia phage T2
GTP
-
 Escherichia phage T2
UDP
-
 Escherichia phage T2
UTP
-
 Escherichia phage T2