Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.7.1.77 extracted from

  • Brunngraber, E.F.; Chargaff, E.
    Purification and properties of a nucleoside phosphotransferase from carrot (1967), J. Biol. Chem., 242, 4834-4840.
    View publication on PubMed
Search for more literature for 2.7.1.77

General Stability

General Stability Organism
dialysis first against EDTA or oxime and then against water produces an inhibition which results in the recovery of 36% of the initial phosphotransferase activity and 65% of the initial phosphatase activity Daucus carota
no loss of activity after dialysis overnight against distilled water Daucus carota

Inhibitors

Inhibitors Comment Organism Structure
Ca2+
-
 Daucus carota
cysteine 2.5 mM, depresses both transferase and phosphatase activity by about 25% Daucus carota
EDTA 0.625 mM, inhibition is reversed by 5 mM MgCl2 Daucus carota
Fe2+
-
 Daucus carota
Fe3+
-
 Daucus carota
glutathione 2.5 mM, depresses both transferase and phosphatase activity by about 25% Daucus carota
Mn2+
-
 Daucus carota
Pb2+
-
 Daucus carota

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ enhances phosphatase activity considerably, but transferase activity to a small extent Daucus carota
Cu2+ enhances phosphatase activity considerably, but transferase activity to a small extent Daucus carota
Mg2+ enhances phosphatase activity considerably, but transferase activity to a small extent Daucus carota

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
additional information
-
 the particle weight of the enzyme as determined by gel filtration varies with pH. At pH 5.0 one species of 45000 Da is detected, at pH 9.5 two species of 44000 Da and 63000 Da are observed Daucus carota

Organism

Organism UniProt Comment Textmining
Daucus carota
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Daucus carota

Source Tissue

Source Tissue Comment Organism Textmining
root
-
 Daucus carota
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Daucus carota

Storage Stability

Storage Stability Organism
-20°C, stable for at least 1 months Daucus carota
4°C, 1 week, 80% of the transferase activity is retained, no loss of hydrolase activity Daucus carota

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2',3')-uridylic acid + uridine about 10% of the activity with phenyl phosphate Daucus carota uridine + UMP
-
 ?
3'-AMP + uridine 70% of the activity with phenyl phosphate Daucus carota adenosine + UMP
-
 ?
5'-adenylic acid + uridine 70% of the activity with phenyl phosphate Daucus carota adenosine + UMP
-
 ?
beta-glycerophosphate + uridine about 15% of the activity with phenyl phosphate Daucus carota glycerol + UMP
-
 ?
D-ribose 5-phosphate + uridine about 30% of the activity with phenyl phosphate Daucus carota D-ribose + UMP
-
 ?
additional information the enzyme also exhibits phosphatase activity Daucus carota ?
-
 ?
additional information protein carries both transfer and hydrolytic functions Daucus carota ?
-
 ?
phenyl phosphate + adenosine
-
 Daucus carota AMP + phenol
-
 ?
phenyl phosphate + cytidine
-
 Daucus carota CMP + phenol
-
 ?
phenyl phosphate + deoxyadenosine
-
 Daucus carota dAMP + phenol
-
 ?
phenyl phosphate + deoxycytidine
-
 Daucus carota dCMP + phenol
-
 ?
phenyl phosphate + deoxyguanosine
-
 Daucus carota dGMP + phenol
-
 ?
phenyl phosphate + deoxyuridine
-
 Daucus carota dUMP + phenol
-
 ?
phenyl phosphate + guanosine
-
 Daucus carota GMP + phenol
-
 ?
phenyl phosphate + thymidine
-
 Daucus carota TMP + phenol
-
 ?
phenyl phosphate + uridine
-
 Daucus carota 5'-UMP + phenol
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
 reaction with phenylphosphate and uridine or deoxycytidine Daucus carota

pH Range

pH Minimum pH Maximum Comment Organism
4 7 pH 4.0: about 25% of maximal activity in the reaction with phenyl phosphate and uridine, about 40% of maximal activity with phenyl phosphate and deoxycytidine , pH 7.0: about 20% of maximal activity in the reaction with phenyl phosphate and uridine, about 50% of maximal activity with phenyl phosphate and deoxycytidine Daucus carota