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Literature summary for 2.7.1.74 extracted from

  • Kozai, Y.; Sonoda, S.; Kobayashi, S.; Sugino, Y.
    Metabolism in deoxyribonucleotides. IV. Purification and properties of deoxycytidine kinase of calf thymus (1972), J. Biochem., 71, 485-496.
    View publication on PubMed
Search for more literature for 2.7.1.74

Inhibitors

Inhibitors Comment Organism Structure
ADP
-
 Bos taurus
CDP strong Bos taurus
CTP
-
 Bos taurus
cytidine not Bos taurus
dADP strong Bos taurus
dATP strong Bos taurus
dCDP strong, ATP as substrate Bos taurus
dCMP ATP as substrate; product inhibition, strong Bos taurus
dCTP ATP as substrate Bos taurus
deoxycytidine cytidine as substrate; deoxyguanosine as substrate Bos taurus
deoxyguanosine not Bos taurus
dGDP strong Bos taurus
dGTP
-
 Bos taurus
dTDP strong Bos taurus
dTTP
-
 Bos taurus
dUDP strong Bos taurus
GDP
-
 Bos taurus
GTP
-
 Bos taurus
Tris-maleate buffer
-
 Bos taurus
UDP strong Bos taurus
UTP
-
 Bos taurus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information kinetic study Bos taurus
additional information
-
 additional information linear kinetics with deoxyguanosine or cytidine, bimodal kinetics with deoxycytidine as substrate Bos taurus
1.4
-
 cytidine 37°C, pH 8.0 Bos taurus
3
-
 deoxyguanosine 37°C, pH 8.0 Bos taurus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Bos taurus 5829
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ activation Bos taurus
Mg2+ below: decrease of activity Bos taurus
Mg2+ Mg2+/ATP complex is the reactive species Bos taurus
Mg2+ maximal activity at a ATP:Mg2+-ratio of 1:1 Bos taurus
Mn2+ requirement Bos taurus
Ni2+ activation Bos taurus
Zn2+ activation Bos taurus

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial Bos taurus

Source Tissue

Source Tissue Comment Organism Textmining
thymus
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.00523
-
 37°C, pH 8.0 Bos taurus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 2'-deoxycytidine
-
 Bos taurus ADP + 5'-dCMP
-
 ?
ATP + cytidine
-
 Bos taurus ADP + ?
-
 ?
CTP + 2'-deoxycytidine
-
 Bos taurus CDP + 2'-deoxy-CMP
-
 ?
dGTP + 2'-deoxycytidine best phosphate donor Bos taurus dGDP + 2'-deoxy-CMP
-
 ?
dTTP + 2'-deoxycytidine
-
 Bos taurus dTDP + 2'-deoxy-CMP
-
 ?
GTP + 2'-deoxycytidine
-
 Bos taurus GDP + 2'-deoxy-CMP
-
 ?
NTP + deoxycytidine no substrate: dCTP Bos taurus NDP + dCMP
-
 ?
NTP + deoxycytidine no substrate: guanosine Bos taurus NDP + dCMP
-
 ?
NTP + deoxycytidine in order of decreasing activity: dGTP, CTP, ATP, dATP, dTTP, UTP, GTP Bos taurus NDP + dCMP
-
 ?
NTP + deoxycytidine no substrate: adenosine, uridine Bos taurus NDP + dCMP
-
 ?
NTP + deoxycytidine no substrate: thymidine Bos taurus NDP + dCMP
-
 ?
NTP + deoxyguanosine
-
 Bos taurus NDP + dGMP
-
 ?
UTP + 2'-deoxycytidine
-
 Bos taurus UDP + 2'-deoxy-CMP
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Bos taurus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Bos taurus

pH Range

pH Minimum pH Maximum Comment Organism
7 10 about 80% of maximal activity at pH 7 and about 65% of maximal activity at pH 10 Bos taurus