Cloned (Comment) | Organism |
---|---|
gene expression patterns of isoforms, AtSK1 and AtSK2 showing a signature of regulatory subfunctionalization | Arabidopsis thaliana |
Crystallization (Comment) | Organism |
---|---|
heat-stable isozyme AtSK2, X-ray diffraction structure determinaion and analysis at 2.35 A resolution | Arabidopsis thaliana |
Protein Variants | Comment | Organism |
---|---|---|
K138N | AtSK1 mutation relative to AtSK2 predicted to confer substantial stabilizing effect | Arabidopsis thaliana |
K87R | AtSK1 mutation relative to AtSK2 predicted to confer substantial stabilizing effect | Arabidopsis thaliana |
L233F | AtSK1 mutation relative to AtSK2 predicted to confer substantial stabilizing effect | Arabidopsis thaliana |
N234K | AtSK1 mutation relative to AtSK2 predicted to confer substantial stabilizing effect | Arabidopsis thaliana |
R120K | AtSK1 mutation relative to AtSK2 predicted to confer substantial stabilizing effect | Arabidopsis thaliana |
S152N | AtSK1 mutation relative to AtSK2 predicted to confer substantial stabilizing effect | Arabidopsis thaliana |
S251N | AtSK1 mutation relative to AtSK2 predicted to confer substantial stabilizing effect | Arabidopsis thaliana |
T230S | AtSK1 mutation relative to AtSK2 predicted to confer substantial stabilizing effect | Arabidopsis thaliana |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required, Mg2+ coordinating residues are Thr112 and Asp128 | Arabidopsis thaliana |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + shikimate | Arabidopsis thaliana | - |
ADP + 3-phosphoshikimate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | Q8GY88 | two isozymes, AtSK1 and AtSK2, AatSK1 is heat-inducible | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + shikimate | - |
Arabidopsis thaliana | ADP + 3-phosphoshikimate | - |
? | |
additional information | AtSK2 shikimate binding residues include Gly177, Gly178, Gly179, and Asp130 of the Walker B motif and Phe146, Phe154, Glu158, Arg155, and Arg243 | Arabidopsis thaliana | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | AtSK2 adopts an alpha-beta-alpha fold with a central sheet of five parallel beta-strands flanked by two layers of alpha-helices. The AtSK2 structure can be divided into four domains: the reduced CORE domain comprising the central beta-sheet and flanking alpha-helices, the nucleotide binding domain, which includes the phosphate binding loop (P-loop/Walker-A motif), the disordered LID domain, which contains catalytic and substrate binding residues, and the extended shikimate binding domain, which includes a modified SK-type Walker B motif and several other substrate binding residues. Computational analysis of isozyme AtSK2 structure, overview | Arabidopsis thaliana |
Synonyms | Comment | Organism |
---|---|---|
AtSK1 | - |
Arabidopsis thaliana |
AtSK2 | - |
Arabidopsis thaliana |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
isozyme AtSK2 is highly unstable and becomes inactivated at 37°C, whereas the heat-induced isoform, AtSK1, is thermostable and fully active at 37°C | Arabidopsis thaliana |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Arabidopsis thaliana |
Organism | Comment | Expression |
---|---|---|
Arabidopsis thaliana | expression of plant shikimate kinase is induced under specific conditions of environmental stress and developmental requirements in an isoform-specific manner | up |
General Information | Comment | Organism |
---|---|---|
evolution | AtSK2 belongs to the nucleoside monophosphate kinase family | Arabidopsis thaliana |
metabolism | shikimate kinase catalyzes an intermediate step in the shikimate pathway to aromatic amino acid biosynthesis | Arabidopsis thaliana |
additional information | mechanism of thermal regulation, computational analysis of AtSK1 and AtSK2 structural variation, overview | Arabidopsis thaliana |