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Literature summary for 2.7.1.68 extracted from

  • Giudici, M.L.; Emson, P.C.; Irvine, R.F.
    A novel neuronal-specific splice variant of Type I phosphatidylinositol 4-phosphate 5-kinase isoform gamma (2004), Biochem. J., 379, 489-496.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.1.68

Cloned(Commentary)

Cloned (Comment) Organism
identification of splicing form c of type I phosphatidylinositol 4-phosphate 5-kinase isoform gamma possessing additional 26 amino acids at the C-terminus from a hippocampus library, DNA and amino acid sequence determination and analysis, overvexpression of the catalytically inactive mutants D316K and K188A of isozyme gamma splicing form c in rat cerebellar granule cells, expression of isozyme gamma splicing form a, expression of wild-type and FLAG-tagged isozyme gamma splicing form c in COS-7 cells Rattus norvegicus

Protein Variants

Protein Variants Comment Organism
D316K site-directed mutagenesis, overexpression of the catalytically inactive construct of isozyme gamma splicing form c in rat cerebellar granule cells causes progressive loss of their neuronal processes, not observed with expression of isozyme gamma splicing form a Rattus norvegicus
K188A site-directed mutagenesis, overexpression of the catalytically inactive construct of isozyme gamma splicing form c in rat cerebellar granule cells causes progressive loss of their neuronal processes, not observed with expression of isozyme gamma splicing form a Rattus norvegicus

Metals/Ions

Metals/Ions Comment Organism Structure
KCl at 80 mM Rattus norvegicus
MgCl2 at 10 mM Rattus norvegicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
75600
-
 x * 75600, isozyme splicing form c, SDS-PAGE Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + 1-phosphatidyl-1D-myo-inositol 4-phosphate Rattus norvegicus
-
 ADP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate
-
 ?
additional information Rattus norvegicus splicing form a of the isozyme gamma is inactive, splicing form c is involved in maintenance of some neuronal cellular processes ?
-
 ?

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
 type I phosphatidylinositol 4-phosphate 5-kinase isozyme gamma, 3 splicing forms a-c
-

Purification (Commentary)

Purification (Comment) Organism
wild-type and mutant isozyme gamma, partially by immunoprecipitation and protein G affinity chromatography Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
brain
-
 Rattus norvegicus
-
cerebellum granule cells Rattus norvegicus
-
hippocampus dentate gyrus, pyramidal cells Rattus norvegicus
-
neuron in the pyramidal cells of the hippocampus and of the cerebral cortex, splicing form c of isozyme gamma is neuronal-specific Rattus norvegicus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 1-phosphatidyl-1D-myo-inositol 4-phosphate
-
 Rattus norvegicus ADP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate
-
 ?
additional information splicing form a of the isozyme gamma is inactive, splicing form c is involved in maintenance of some neuronal cellular processes Rattus norvegicus ?
-
 ?

Subunits

Subunits Comment Organism
? x * 75600, isozyme splicing form c, SDS-PAGE Rattus norvegicus

Synonyms

Synonyms Comment Organism
phosphatidylinositol 4-phosphate 5-kinase
-
 Rattus norvegicus
PIPkin
-
 Rattus norvegicus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
 assay at Rattus norvegicus

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Rattus norvegicus