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Literature summary for 2.7.1.68 extracted from

  • Kanaho, Y.; Miyazaki, H.; Yamazaki, M.
    Activation of PI(4)P 5-kinase by small G proteins (2003), Adv. Enzyme Regul., 43, 107-119.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
small G protein ARF ARF activation absolutely requires the presence of phosphatidic acid, which cannot be substituted by phosphatidylserine, phosphatidylethanolamine, or lysophosphatidic acid, but by competitive phosphatidylcholine, reconstitution of the recombinant either isozyme alpha, beta, or gamma with recombinant class II and III ARFs, ARF5 and ARF6, respectively, as well as myristoylated class I ARF in presence of GTPgammaS or GDP, and phosphatidic acid activates the isozymes Mus musculus
small G protein RHO activates the enzyme in neurits, crucial in cell shapig together with the enzyme, overview Mus musculus
small G proteins
-
Bos taurus
small G proteins ARF ARF activation absolutely requires the presence of phosphatidic acid, which cannot be substituted by phosphatidylserine, phosphatidylethanolamine, or lysophosphatidic acid, but by competitive phosphatidylcholine Homo sapiens
small G proteins ARF ARF activation absolutely requires the presence of phosphatidic acid, which cannot be substituted by phosphatidylserine, phosphatidylethanolamine, or lysophosphatidic acid, but by competitive phosphatidylcholine Rattus norvegicus

Cloned(Commentary)

Cloned (Comment) Organism
functional expression in HeLa cells as GFP-fusion protein, translocation to the plasma membrane by coexpression of ARF6 upon stimulation by EGF, coexpression of ARF6 mutant N122I inhibits the membrane ruffling, overview Homo sapiens
overexpression of mutant D266A in N1E-115 cells, expression of isozyme alpha in COS-7 cells, expression of isozyme alpha in Escherichia coli Mus musculus

Protein Variants

Protein Variants Comment Organism
D266A site-directed mtagenesis, mutation of the putative ATP and 1-phosphatidyl-1D-myo-inositol 4-phosphate binding site, cells form extremely extended bipolar neurits even in presence of serum, failed to interfere with cell flattening and neurite extension Mus musculus

Inhibitors

Inhibitors Comment Organism Structure
additional information GTPgammaS shows poor or no activation of the brain cytosol enzyme but acts synergistically with phosphatidic acid Bos taurus
phosphatidic acid 2-3fold activation of the brain cytosol enzyme, 30fold enhancement of the activation by GTPgammaS, even higher activation by addition of recombinant mouse isozyme alpha Bos taurus
small G proteins ARF ARF activation absolutely requires the presence of phosphatidic acid, which cannot be substituted by phosphatidylserine, phosphatidylethanolamine, or lysophophatidic acid, but by competitive phosphatidylcholine, proteins ARF1 and ARF3, from brain cytosol, purification and identification, the 21 kDa G-proteins are activated by GTPgammaS, not by GDP, and activate themselves the PI(4)P 5-kinase Bos taurus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Mus musculus 5829
-
cytosol
-
Homo sapiens 5829
-
cytosol
-
Rattus norvegicus 5829
-
cytosol
-
Bos taurus 5829
-
Golgi apparatus ARF recruits brain cytosol enzyme from the cytosol to the Golgi complex in an in vitro system Bos taurus 5794
-
plasma membrane translocation to the plasma membrane of isozyme alpha is dependent on ARF6 and EGF Mus musculus 5886
-
plasma membrane translocation to the plasma membrane of isozyme alpha is dependent on ARF6 and EGF Rattus norvegicus 5886
-
plasma membrane translocation to the plasma membrane of isozyme beta is dependent on ARF6 and EGF, and is completely inhibited by the recombinant GTP-binding-deficient ARF6 mutant N122I Homo sapiens 5886
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
Mus musculus
Mg2+
-
Homo sapiens
Mg2+
-
Rattus norvegicus
Mg2+
-
Bos taurus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + 1-phosphatidyl-1D-myo-inositol 4-phosphate Mus musculus
-
ADP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate has several important physiologic functions, overview ?
ATP + 1-phosphatidyl-1D-myo-inositol 4-phosphate Bos taurus
-
ADP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate has several important physiologic functions, overview ?
ATP + 1-phosphatidyl-1D-myo-inositol 4-phosphate Homo sapiens
-
ADP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate has several important physiological functions, overview ?
ATP + 1-phosphatidyl-1D-myo-inositol 4-phosphate Rattus norvegicus
-
ADP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate has several important physiological functions, overview ?
additional information Homo sapiens ARF6-dependent enzyme activation plays an important role in signal transduction of membrane ruffling formation through 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate ?
-
?
additional information Rattus norvegicus ARF6-dependent enzyme activation plays an important role in signal transduction of membrane ruffling formation through 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate ?
-
?
additional information Bos taurus ARF6-dependent enzyme activation plays an important role in signal transduction of membrane ruffling formation through 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate ?
-
?
additional information Mus musculus enzyme is important in cell signaling and cell shaping, overview, the enzyme is essential for regulation of neurite modeling, ARF6-dependent enzyme activation plays an important role in signal transduction of membrane ruffling formation through 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate ?
-
?

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-
Homo sapiens
-
3 isozymes alpha, beta, and gamma
-
Mus musculus
-
3 isozymes alpha, beta, and gamma
-
Rattus norvegicus
-
3 isozymes alpha, beta, and gamma
-

Source Tissue

Source Tissue Comment Organism Textmining
brain
-
Bos taurus
-
fibroblast
-
Mus musculus
-
N1E-115 cell neuroblastoma cell Mus musculus
-
neuroblastoma cell
-
Mus musculus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 1-phosphatidyl-1D-myo-inositol 4-phosphate
-
Mus musculus ADP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate
-
?
ATP + 1-phosphatidyl-1D-myo-inositol 4-phosphate
-
Homo sapiens ADP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate
-
?
ATP + 1-phosphatidyl-1D-myo-inositol 4-phosphate
-
Rattus norvegicus ADP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate
-
?
ATP + 1-phosphatidyl-1D-myo-inositol 4-phosphate
-
Bos taurus ADP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate
-
?
ATP + 1-phosphatidyl-1D-myo-inositol 4-phosphate
-
Mus musculus ADP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate has several important physiologic functions, overview ?
ATP + 1-phosphatidyl-1D-myo-inositol 4-phosphate
-
Bos taurus ADP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate has several important physiologic functions, overview ?
ATP + 1-phosphatidyl-1D-myo-inositol 4-phosphate
-
Homo sapiens ADP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate has several important physiological functions, overview ?
ATP + 1-phosphatidyl-1D-myo-inositol 4-phosphate
-
Rattus norvegicus ADP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate has several important physiological functions, overview ?
additional information ARF6-dependent enzyme activation plays an important role in signal transduction of membrane ruffling formation through 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate Homo sapiens ?
-
?
additional information ARF6-dependent enzyme activation plays an important role in signal transduction of membrane ruffling formation through 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate Rattus norvegicus ?
-
?
additional information ARF6-dependent enzyme activation plays an important role in signal transduction of membrane ruffling formation through 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate Bos taurus ?
-
?
additional information enzyme is important in cell signaling and cell shaping, overview, the enzyme is essential for regulation of neurite modeling, ARF6-dependent enzyme activation plays an important role in signal transduction of membrane ruffling formation through 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate Mus musculus ?
-
?

Subunits

Subunits Comment Organism
More the enzyme possesses a central so-called kinase core domain containing the activation loop which is responsible for substrate specificity and subcellular targeting of the enzyme Mus musculus
More the enzyme possesses a central so-called kinase core domain containing the activation loop which is responsible for substrate specificity and subcellular targeting of the enzyme Homo sapiens
More the enzyme possesses a central socalled kinase core domain containing the activation loop which is responsible for substrate specificity and subcellular targeting of the enzyme Rattus norvegicus
More the enzyme possesses a central socalled kinase core domain containing the activation loop which is responsible for substrate specificity and subcellular targeting of the enzyme Bos taurus

Synonyms

Synonyms Comment Organism
PI(4)P 5-kinase
-
Mus musculus
PI(4)P 5-kinase
-
Homo sapiens
PI(4)P 5-kinase
-
Rattus norvegicus
PI(4)P 5-kinase
-
Bos taurus

Cofactor

Cofactor Comment Organism Structure
ATP
-
Mus musculus
ATP
-
Homo sapiens
ATP
-
Rattus norvegicus
ATP
-
Bos taurus