Activating Compound | Comment | Organism | Structure |
---|---|---|---|
small G protein ARF | ARF activation absolutely requires the presence of phosphatidic acid, which cannot be substituted by phosphatidylserine, phosphatidylethanolamine, or lysophosphatidic acid, but by competitive phosphatidylcholine, reconstitution of the recombinant either isozyme alpha, beta, or gamma with recombinant class II and III ARFs, ARF5 and ARF6, respectively, as well as myristoylated class I ARF in presence of GTPgammaS or GDP, and phosphatidic acid activates the isozymes | Mus musculus | |
small G protein RHO | activates the enzyme in neurits, crucial in cell shapig together with the enzyme, overview | Mus musculus | |
small G proteins | - |
Bos taurus | |
small G proteins ARF | ARF activation absolutely requires the presence of phosphatidic acid, which cannot be substituted by phosphatidylserine, phosphatidylethanolamine, or lysophosphatidic acid, but by competitive phosphatidylcholine | Homo sapiens | |
small G proteins ARF | ARF activation absolutely requires the presence of phosphatidic acid, which cannot be substituted by phosphatidylserine, phosphatidylethanolamine, or lysophosphatidic acid, but by competitive phosphatidylcholine | Rattus norvegicus |
Cloned (Comment) | Organism |
---|---|
functional expression in HeLa cells as GFP-fusion protein, translocation to the plasma membrane by coexpression of ARF6 upon stimulation by EGF, coexpression of ARF6 mutant N122I inhibits the membrane ruffling, overview | Homo sapiens |
overexpression of mutant D266A in N1E-115 cells, expression of isozyme alpha in COS-7 cells, expression of isozyme alpha in Escherichia coli | Mus musculus |
Protein Variants | Comment | Organism |
---|---|---|
D266A | site-directed mtagenesis, mutation of the putative ATP and 1-phosphatidyl-1D-myo-inositol 4-phosphate binding site, cells form extremely extended bipolar neurits even in presence of serum, failed to interfere with cell flattening and neurite extension | Mus musculus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | GTPgammaS shows poor or no activation of the brain cytosol enzyme but acts synergistically with phosphatidic acid | Bos taurus | |
phosphatidic acid | 2-3fold activation of the brain cytosol enzyme, 30fold enhancement of the activation by GTPgammaS, even higher activation by addition of recombinant mouse isozyme alpha | Bos taurus | |
small G proteins ARF | ARF activation absolutely requires the presence of phosphatidic acid, which cannot be substituted by phosphatidylserine, phosphatidylethanolamine, or lysophophatidic acid, but by competitive phosphatidylcholine, proteins ARF1 and ARF3, from brain cytosol, purification and identification, the 21 kDa G-proteins are activated by GTPgammaS, not by GDP, and activate themselves the PI(4)P 5-kinase | Bos taurus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Mus musculus | 5829 | - |
cytosol | - |
Homo sapiens | 5829 | - |
cytosol | - |
Rattus norvegicus | 5829 | - |
cytosol | - |
Bos taurus | 5829 | - |
Golgi apparatus | ARF recruits brain cytosol enzyme from the cytosol to the Golgi complex in an in vitro system | Bos taurus | 5794 | - |
plasma membrane | translocation to the plasma membrane of isozyme alpha is dependent on ARF6 and EGF | Mus musculus | 5886 | - |
plasma membrane | translocation to the plasma membrane of isozyme alpha is dependent on ARF6 and EGF | Rattus norvegicus | 5886 | - |
plasma membrane | translocation to the plasma membrane of isozyme beta is dependent on ARF6 and EGF, and is completely inhibited by the recombinant GTP-binding-deficient ARF6 mutant N122I | Homo sapiens | 5886 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Mus musculus | |
Mg2+ | - |
Homo sapiens | |
Mg2+ | - |
Rattus norvegicus | |
Mg2+ | - |
Bos taurus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 1-phosphatidyl-1D-myo-inositol 4-phosphate | Mus musculus | - |
ADP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate | 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate has several important physiologic functions, overview | ? | |
ATP + 1-phosphatidyl-1D-myo-inositol 4-phosphate | Bos taurus | - |
ADP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate | 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate has several important physiologic functions, overview | ? | |
ATP + 1-phosphatidyl-1D-myo-inositol 4-phosphate | Homo sapiens | - |
ADP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate | 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate has several important physiological functions, overview | ? | |
ATP + 1-phosphatidyl-1D-myo-inositol 4-phosphate | Rattus norvegicus | - |
ADP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate | 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate has several important physiological functions, overview | ? | |
additional information | Homo sapiens | ARF6-dependent enzyme activation plays an important role in signal transduction of membrane ruffling formation through 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate | ? | - |
? | |
additional information | Rattus norvegicus | ARF6-dependent enzyme activation plays an important role in signal transduction of membrane ruffling formation through 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate | ? | - |
? | |
additional information | Bos taurus | ARF6-dependent enzyme activation plays an important role in signal transduction of membrane ruffling formation through 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate | ? | - |
? | |
additional information | Mus musculus | enzyme is important in cell signaling and cell shaping, overview, the enzyme is essential for regulation of neurite modeling, ARF6-dependent enzyme activation plays an important role in signal transduction of membrane ruffling formation through 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Homo sapiens | - |
3 isozymes alpha, beta, and gamma | - |
Mus musculus | - |
3 isozymes alpha, beta, and gamma | - |
Rattus norvegicus | - |
3 isozymes alpha, beta, and gamma | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | - |
Bos taurus | - |
fibroblast | - |
Mus musculus | - |
N1E-115 cell | neuroblastoma cell | Mus musculus | - |
neuroblastoma cell | - |
Mus musculus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 1-phosphatidyl-1D-myo-inositol 4-phosphate | - |
Mus musculus | ADP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate | - |
? | |
ATP + 1-phosphatidyl-1D-myo-inositol 4-phosphate | - |
Homo sapiens | ADP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate | - |
? | |
ATP + 1-phosphatidyl-1D-myo-inositol 4-phosphate | - |
Rattus norvegicus | ADP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate | - |
? | |
ATP + 1-phosphatidyl-1D-myo-inositol 4-phosphate | - |
Bos taurus | ADP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate | - |
? | |
ATP + 1-phosphatidyl-1D-myo-inositol 4-phosphate | - |
Mus musculus | ADP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate | 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate has several important physiologic functions, overview | ? | |
ATP + 1-phosphatidyl-1D-myo-inositol 4-phosphate | - |
Bos taurus | ADP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate | 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate has several important physiologic functions, overview | ? | |
ATP + 1-phosphatidyl-1D-myo-inositol 4-phosphate | - |
Homo sapiens | ADP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate | 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate has several important physiological functions, overview | ? | |
ATP + 1-phosphatidyl-1D-myo-inositol 4-phosphate | - |
Rattus norvegicus | ADP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate | 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate has several important physiological functions, overview | ? | |
additional information | ARF6-dependent enzyme activation plays an important role in signal transduction of membrane ruffling formation through 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate | Homo sapiens | ? | - |
? | |
additional information | ARF6-dependent enzyme activation plays an important role in signal transduction of membrane ruffling formation through 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate | Rattus norvegicus | ? | - |
? | |
additional information | ARF6-dependent enzyme activation plays an important role in signal transduction of membrane ruffling formation through 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate | Bos taurus | ? | - |
? | |
additional information | enzyme is important in cell signaling and cell shaping, overview, the enzyme is essential for regulation of neurite modeling, ARF6-dependent enzyme activation plays an important role in signal transduction of membrane ruffling formation through 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate | Mus musculus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | the enzyme possesses a central so-called kinase core domain containing the activation loop which is responsible for substrate specificity and subcellular targeting of the enzyme | Mus musculus |
More | the enzyme possesses a central so-called kinase core domain containing the activation loop which is responsible for substrate specificity and subcellular targeting of the enzyme | Homo sapiens |
More | the enzyme possesses a central socalled kinase core domain containing the activation loop which is responsible for substrate specificity and subcellular targeting of the enzyme | Rattus norvegicus |
More | the enzyme possesses a central socalled kinase core domain containing the activation loop which is responsible for substrate specificity and subcellular targeting of the enzyme | Bos taurus |
Synonyms | Comment | Organism |
---|---|---|
PI(4)P 5-kinase | - |
Mus musculus |
PI(4)P 5-kinase | - |
Homo sapiens |
PI(4)P 5-kinase | - |
Rattus norvegicus |
PI(4)P 5-kinase | - |
Bos taurus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Mus musculus | |
ATP | - |
Homo sapiens | |
ATP | - |
Rattus norvegicus | |
ATP | - |
Bos taurus |