Any feedback?
Literature summary for 2.7.1.63 extracted from
- Cg2091 encodes a polyphosphate/ATP-dependent glucokinase of Corynebacterium glutamicum (2010), Appl. Microbiol. Biotechnol., 87, 703-713.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | no effect by fructose 1,6-bisphosphate, xylose, cAMP, UDP glucose, and glucose 1,6-bisphosphate | Corynebacterium glutamicum |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene cg2091 or ppgK, sequence comparisons and phylogenetic analysis, expression of His-tagged enzyme in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain DH5alpha | Corynebacterium glutamicum |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ADP | - |
Corynebacterium glutamicum |  |
| AMP | - |
Corynebacterium glutamicum | |
| additional information | no effect by fructose 1,6-bisphosphate, xylose, cAMP, UDP glucose, and glucose 1,6-bisphosphate | Corynebacterium glutamicum |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.1 | - |
(phosphate)65 | pH 7.0, temperature not specified in the publication | Corynebacterium glutamicum | |
| 0.1 | - |
(phosphate)120 | pH 7.0, temperature not specified in the publication | Corynebacterium glutamicum | |
| 0.2 | - |
(phosphate)45 | pH 7.0, temperature not specified in the publication | Corynebacterium glutamicum | |
| 0.3 | - |
(phosphate)25 | pH 7.0, temperature not specified in the publication | Corynebacterium glutamicum | |
| 0.3 | - |
(phosphate)75 | pH 7.0, temperature not specified in the publication | Corynebacterium glutamicum | |
| 0.4 | - |
(phosphate)20 | pH 7.0, temperature not specified in the publication | Corynebacterium glutamicum | |
| 0.5 | - |
(phosphate)4 | pH 7.0, temperature not specified in the publication | Corynebacterium glutamicum | |
| 0.6 | - |
(phosphate)15 | pH 7.0, temperature not specified in the publication | Corynebacterium glutamicum | |
| 0.8 | - |
ITP | pH 7.0, temperature not specified in the publication | Corynebacterium glutamicum | |
| 0.8 | - |
(phosphate)5 | pH 7.0, temperature not specified in the publication | Corynebacterium glutamicum | |
| 1.5 | - |
UTP | pH 7.0, temperature not specified in the publication | Corynebacterium glutamicum | |
| 3.5 | - |
GTP | pH 7.0, temperature not specified in the publication | Corynebacterium glutamicum | |
| 3.9 | - |
CTP | pH 7.0, temperature not specified in the publication | Corynebacterium glutamicum | |
| 6 | - |
ATP | pH 7.0, temperature not specified in the publication | Corynebacterium glutamicum | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| additional information | the enzyme is a component of PolyP containing granular (voluntin) in Corynebacterium glutamicum | Corynebacterium glutamicum | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 26700 | - |
2 * 26700, about, sequence calculation | Corynebacterium glutamicum |
| 67800 | - |
recombinant detagged enzyme, gel filtration | Corynebacterium glutamicum |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (phosphate)n + D-glucose | Corynebacterium glutamicum | - |
(phosphate)n-1 + D-glucose 6-phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Corynebacterium glutamicum | A0A160PRJ4 | gene cg2091 or ppgK | - |
| Corynebacterium glutamicum ATCC 13032 | A0A160PRJ4 | gene cg2091 or ppgK | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and His-tag cleavage with factor Xa | Corynebacterium glutamicum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (phosphate)120 + D-glucose | - |
Corynebacterium glutamicum | (phosphate)119 + D-glucose 6-phosphate | - |
? | |
| (phosphate)15 + D-glucose | - |
Corynebacterium glutamicum | (phosphate)14 + D-glucose 6-phosphate | - |
? | |
| (phosphate)20 + D-glucose | - |
Corynebacterium glutamicum | (phosphate)19 + D-glucose 6-phosphate | - |
? | |
| (phosphate)25 + D-glucose | - |
Corynebacterium glutamicum | (phosphate)24 + D-glucose 6-phosphate | - |
? | |
| (phosphate)4 + D-glucose | - |
Corynebacterium glutamicum | (phosphate)3 + D-glucose 6-phosphate | - |
? | |
| (phosphate)45 + D-glucose | - |
Corynebacterium glutamicum | (phosphate)44 + D-glucose 6-phosphate | - |
? | |
| (phosphate)5 + D-glucose | - |
Corynebacterium glutamicum | (phosphate)4 + D-glucose 6-phosphate | - |
? | |
| (phosphate)65 + D-glucose | - |
Corynebacterium glutamicum | (phosphate)64 + D-glucose 6-phosphate | - |
? | |
| (phosphate)75 + D-glucose | - |
Corynebacterium glutamicum | (phosphate)74 + D-glucose 6-phosphate | - |
? | |
| (phosphate)n + D-glucose | - |
Corynebacterium glutamicum | (phosphate)n-1 + D-glucose 6-phosphate | - |
? | |
| ATP + D-glucose | see also glucokinase, EC 2.7.1.2 | Corynebacterium glutamicum | ADP + D-glucose 6-phosphate | - |
? | |
| ATP + D-glucose | see also glucokinase, EC 2.7.1.2 | Corynebacterium glutamicum ATCC 13032 | ADP + D-glucose 6-phosphate | - |
? | |
| CTP + D-glucose | - |
Corynebacterium glutamicum | CDP + D-glucose 6-phosphate | - |
? | |
| CTP + D-glucose | - |
Corynebacterium glutamicum ATCC 13032 | CDP + D-glucose 6-phosphate | - |
? | |
| GTP + D-glucose | - |
Corynebacterium glutamicum | GDP + D-glucose 6-phosphate | - |
? | |
| ITP + D-glucose | - |
Corynebacterium glutamicum | IDP + D-glucose 6-phosphate | - |
? | |
| ITP + D-glucose | - |
Corynebacterium glutamicum ATCC 13032 | IDP + D-glucose 6-phosphate | - |
? | |
| additional information | PolyP is highly preferred over ATP and other NTPs as substrate and with respect to the tested PolyPs differing in chain length. The protein is most active with PolyP75 | Corynebacterium glutamicum | ? | - |
? | |
| additional information | PolyP is highly preferred over ATP and other NTPs as substrate and with respect to the tested PolyPs differing in chain length. The protein is most active with PolyP75 | Corynebacterium glutamicum ATCC 13032 | ? | - |
? | |
| UTP + D-glucose | - |
Corynebacterium glutamicum | UDP + D-glucose 6-phosphate | - |
? | |
| UTP + D-glucose | - |
Corynebacterium glutamicum ATCC 13032 | UDP + D-glucose 6-phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homodimer | 2 * 26700, about, sequence calculation | Corynebacterium glutamicum |
| More | PolyP supports the formation of homodimers, the active form of PPGK | Corynebacterium glutamicum |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| PolyP-dependent glucokinase | - |
Corynebacterium glutamicum |
| polyphosphate-dependent glucokinase | - |
Corynebacterium glutamicum |
| PPGK | - |
Corynebacterium glutamicum |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 40 | purified recombinant enzyme, 100% remaining activity after 1 h | Corynebacterium glutamicum |
| 50 | - |
purified recombinant enzyme, 12% remaining activity after 1 h | Corynebacterium glutamicum |
| 60 | - |
purified recombinant enzyme, no remaining activity after 5 min | Corynebacterium glutamicum |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.1 | - |
CTP | pH 7.0, temperature not specified in the publication | Corynebacterium glutamicum | |
| 0.2 | - |
(phosphate)5 | pH 7.0, temperature not specified in the publication | Corynebacterium glutamicum | |
| 0.3 | - |
UTP | pH 7.0, temperature not specified in the publication | Corynebacterium glutamicum | |
| 0.4 | - |
ATP | pH 7.0, temperature not specified in the publication | Corynebacterium glutamicum | |
| 1.3 | - |
GTP | pH 7.0, temperature not specified in the publication | Corynebacterium glutamicum | |
| 2.5 | - |
(phosphate)4 | pH 7.0, temperature not specified in the publication | Corynebacterium glutamicum | |
| 3.3 | - |
ITP | pH 7.0, temperature not specified in the publication | Corynebacterium glutamicum | |
| 6 | - |
(phosphate)15 | pH 7.0, temperature not specified in the publication | Corynebacterium glutamicum | |
| 8.3 | - |
(phosphate)45 | pH 7.0, temperature not specified in the publication | Corynebacterium glutamicum | |
| 8.5 | - |
(phosphate)20 | pH 7.0, temperature not specified in the publication | Corynebacterium glutamicum | |
| 10 | - |
(phosphate)25 | pH 7.0, temperature not specified in the publication | Corynebacterium glutamicum | |
| 11.3 | - |
(phosphate)120 | pH 7.0, temperature not specified in the publication | Corynebacterium glutamicum | |
| 11.6 | - |
(phosphate)65 | pH 7.0, temperature not specified in the publication | Corynebacterium glutamicum | |
| 15.4 | - |
(phosphate)75 | pH 7.0, temperature not specified in the publication | Corynebacterium glutamicum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
- |
Corynebacterium glutamicum |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5.7 | 8.4 | 84% activity at pH 6.4 in 50 mM MES and 73% activity at pH 8.4 in 50 mM glycylglycine | Corynebacterium glutamicum |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 11 | - |
ADP | pH 7.0, temperature not specified in the publication | Corynebacterium glutamicum | |
| 15 | - |
AMP | pH 7.0, temperature not specified in the publication | Corynebacterium glutamicum | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | phylogenetic analysis | Corynebacterium glutamicum |
| malfunction | a ppgK deletion mutant shows slowed growth in minimal medium with maltose as sole carbon source or under phosphate starvation. Moreover, in minimal medium containing 2 to 4% w/v glucose as carbon source, DELTAppgK grows to lower final biomass concentrations than the wild-type. Growth of a ppgK overexpressing strain is increased as compared to wild-type and empty vector control, respectively | Corynebacterium glutamicum |
| physiological function | presence of PPGK entails a growth advantage | Corynebacterium glutamicum |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.03 | - |
CTP | pH 7.0, temperature not specified in the publication | Corynebacterium glutamicum | |
| 0.07 | - |
ATP | pH 7.0, temperature not specified in the publication | Corynebacterium glutamicum | |
| 0.2 | - |
UTP | pH 7.0, temperature not specified in the publication | Corynebacterium glutamicum | |
| 0.3 | - |
(phosphate)5 | pH 7.0, temperature not specified in the publication | Corynebacterium glutamicum | |
| 0.4 | - |
GTP | pH 7.0, temperature not specified in the publication | Corynebacterium glutamicum | |
| 4.1 | - |
ITP | pH 7.0, temperature not specified in the publication | Corynebacterium glutamicum | |
| 5 | - |
(phosphate)4 | pH 7.0, temperature not specified in the publication | Corynebacterium glutamicum | |
| 10 | - |
(phosphate)15 | pH 7.0, temperature not specified in the publication | Corynebacterium glutamicum | |
| 21.3 | - |
(phosphate)20 | pH 7.0, temperature not specified in the publication | Corynebacterium glutamicum | |
| 33.3 | - |
(phosphate)25 | pH 7.0, temperature not specified in the publication | Corynebacterium glutamicum | |
| 41.5 | - |
(phosphate)45 | pH 7.0, temperature not specified in the publication | Corynebacterium glutamicum | |
| 51.3 | - |
(phosphate)75 | pH 7.0, temperature not specified in the publication | Corynebacterium glutamicum | |
| 113 | - |
(phosphate)120 | pH 7.0, temperature not specified in the publication | Corynebacterium glutamicum | |
| 116 | - |
(phosphate)65 | pH 7.0, temperature not specified in the publication | Corynebacterium glutamicum |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
