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Literature summary for 2.7.1.61 extracted from
- Acyl phosphate: hexose phosphotransferase. Purification and properties of the enzyme from Aerobacter aerogenes and evidence for its common identity with hexose phosphate: hexose phosphotransferase (1967), Arch. Biochem. Biophys., 120, 322-331.
General Stability
| General Stability | Organism |
|---|---|
| stable to repeated freezing and thawing | Klebsiella aerogenes |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| additional information | not inhibitory: EDTA | Klebsiella aerogenes |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.16 | - |
D-glucose | pH 7.5, 25°C | Klebsiella aerogenes |  |
| 0.4 | - |
acetyl phosphate | carbamoyl phosphate, D-mannose 6-phosphate, pH 7.5, 25°C | Klebsiella aerogenes | |
| 2 | - |
D-ribose 5-phosphate | pH 7.5, 25°C | Klebsiella aerogenes | |
| 12 | - |
D-mannose | pH 7.5, 25°C | Klebsiella aerogenes | |
| 67 | - |
mannitol | pH 7.5, 25°C | Klebsiella aerogenes | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| additional information | no stimulation by addition of various cations | Klebsiella aerogenes |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acyl phosphate + D-hexose | Klebsiella aerogenes | - |
an acid + D-hexose phosphate | - |
? | |
| D-glucose + acetyl phosphate | Klebsiella aerogenes | - |
D-glucose 6-phosphate + acetate | - |
? | |
| D-glucose + acetyl phosphate | Klebsiella aerogenes PRL-R3 | - |
D-glucose 6-phosphate + acetate | - |
? | |
| D-glucose + D-mannose 6-phosphate | Klebsiella aerogenes | - |
D-glucose 6-phosphate + D-mannose | - |
? | |
| D-glucose + D-mannose 6-phosphate | Klebsiella aerogenes PRL-R3 | - |
D-glucose 6-phosphate + D-mannose | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Klebsiella aerogenes | - |
PRL-R3 | - |
| Klebsiella aerogenes PRL-R3 | - |
PRL-R3 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Klebsiella aerogenes |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1.3 | - |
pH 7.5, 25°C | Klebsiella aerogenes |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acyl phosphate + D-hexose | - |
Klebsiella aerogenes | an acid + D-hexose phosphate | - |
? | |
| D-fructose + acetyl phosphate | - |
Klebsiella aerogenes | D-fructose 1-phosphate + D-fructose 6-phosphate + acetate | - |
? | |
| D-glucose + acetyl phosphate | - |
Klebsiella aerogenes | D-glucose 6-phosphate + acetate | - |
? | |
| D-glucose + acetyl phosphate | - |
Klebsiella aerogenes PRL-R3 | D-glucose 6-phosphate + acetate | - |
? | |
| D-glucose + carbamoyl phosphate | - |
Klebsiella aerogenes | D-glucose 6-phosphate + carbamate | - |
? | |
| D-glucose + carbamoyl phosphate | - |
Klebsiella aerogenes PRL-R3 | D-glucose 6-phosphate + carbamate | - |
? | |
| D-glucose + D-fructose 1-phosphate | 15% of the activity with acetyl phosphate or carbamoyl phosphate | Klebsiella aerogenes | D-glucose 6-phosphate + D-fructose | - |
? | |
| D-glucose + D-fructose 6-phosphate | 25% of the activity with acetyl phosphate or carbamoyl phosphate | Klebsiella aerogenes | D-glucose 6-phosphate + D-fructose | - |
? | |
| D-glucose + D-glucose 1-phosphate | 5% of the activity with acetyl phosphate or carbamoyl phosphate | Klebsiella aerogenes | D-glucose 6-phosphate + D-glucose | - |
? | |
| D-glucose + D-mannose 6-phosphate | - |
Klebsiella aerogenes | D-glucose 6-phosphate + D-mannose | - |
? | |
| D-glucose + D-mannose 6-phosphate | 71% of the activity with acetyl phosphate or carbamoyl phosphate | Klebsiella aerogenes | D-glucose 6-phosphate + D-mannose | - |
? | |
| D-glucose + D-mannose 6-phosphate | - |
Klebsiella aerogenes PRL-R3 | D-glucose 6-phosphate + D-mannose | - |
? | |
| D-glucose + D-mannose 6-phosphate | 71% of the activity with acetyl phosphate or carbamoyl phosphate | Klebsiella aerogenes PRL-R3 | D-glucose 6-phosphate + D-mannose | - |
? | |
| D-glucose + D-ribose 5-phosphate | 18% of the activity with acetyl phosphate or carbamoyl phosphate | Klebsiella aerogenes | D-glucose 6-phosphate + D-ribose | - |
? | |
| D-glucose + D-sorbitol 6-phosphate | 2.5% of the activity with acetyl phosphate or carbamoyl phosphate | Klebsiella aerogenes | D-glucose 6-phosphate + D-sorbitol | - |
? | |
| D-mannitol + acetyl phosphate | - |
Klebsiella aerogenes | ? | - |
? | |
| D-mannose + acetyl phosphate | - |
Klebsiella aerogenes | D-mannose 6-phosphate + acetate | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9 | - |
- |
Klebsiella aerogenes |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | 9 | pH 7.5: 50% of maximum activity, pH 9: maximum activity | Klebsiella aerogenes |
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Release 2023.1 (January 2023)
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