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Literature summary for 2.7.1.6 extracted from

  • Thoden, J.B.; Sellick, C.A.; Timson, D.J.; Reece, R.J.; Holden, H.M.
    Molecular structure of Saccharomyces cerevisiae Gal1p, a bifunctional galactokinase and transcriptional inducer (2005), J. Biol. Chem., 280, 36905-36911.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli DH5alpha cells Saccharomyces cerevisiae

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapour diffusion method using 18-25% poly(ethylene glycol) 8000 containing 200 mM LiCl buffered with 100 mM MES3 (pH 6.0) and enzyme concentrations of 15.0 mg/ml and 5 mM Mg-adenosine 5'-(beta, gamma-imido)triphosphate Saccharomyces cerevisiae

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
nickel-nitrilotriacetic acid-agarose chromatography and Resource-Q column chromatography Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + D-galactose
-
Saccharomyces cerevisiae ADP + alpha-D-galactose 1-phosphate
-
?

Subunits

Subunits Comment Organism
monomer x-ray crystallography Saccharomyces cerevisiae

Synonyms

Synonyms Comment Organism
Gal1p
-
Saccharomyces cerevisiae

Cofactor

Cofactor Comment Organism Structure
ATP
-
Saccharomyces cerevisiae