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Literature summary for 2.7.1.59 extracted from
- Identification of an N-acetylglucosamine kinase essential for UDP-N-acetylglucosamine salvage synthesis in Arabidopsis (2015), FEBS Lett., 589, 3258-3262.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli M15 cells | Arabidopsis thaliana |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.046 | - |
N-acetyl-D-glucosamine | at pH 7.5 and 25°C | Arabidopsis thaliana |  |
| 0.152 | - |
ATP | at pH 7.5 and 25°C | Arabidopsis thaliana | |
| 0.268 | - |
N-acetyl-D-galactosamine | at pH 7.5 and 25°C | Arabidopsis thaliana | |
| 55.56 | - |
D-glucose | at pH 7.5 and 25°C | Arabidopsis thaliana | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 35000 | - |
x * 35000, SDS-PAGE | Arabidopsis thaliana |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + N-acetyl-D-glucosamine | Arabidopsis thaliana | - |
ADP + N-acetyl-D-glucosamine 6-phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Arabidopsis thaliana | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Talon metal affinity resin column chromatography, and gel filtration | Arabidopsis thaliana |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + D-glucose | - |
Arabidopsis thaliana | ADP + D-glucose 6-phosphate | - |
? | |
| ATP + N-acetyl-D-galactosamine | - |
Arabidopsis thaliana | ADP + N-acetyl-D-galactosamine 6-phosphate | - |
? | |
| ATP + N-acetyl-D-glucosamine | - |
Arabidopsis thaliana | ADP + N-acetyl-D-glucosamine 6-phosphate | - |
? | |
| ATP + N-acetyl-D-glucosamine | highly specific for this substrate | Arabidopsis thaliana | ADP + N-acetyl-D-glucosamine 6-phosphate | - |
? | |
| additional information | no activity with N-acetyl-D-mannose, D-mannose, D-galactose, and D-glucosamine | Arabidopsis thaliana | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 35000, SDS-PAGE | Arabidopsis thaliana |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| GlcNAc kinase | - |
Arabidopsis thaliana |
| GNK | - |
Arabidopsis thaliana |
| N-acetyl-D-glucosamine kinase | - |
Arabidopsis thaliana |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.161 | - |
N-acetyl-D-galactosamine | at pH 7.5 and 25°C | Arabidopsis thaliana | |
| 1.586 | - |
ATP | at pH 7.5 and 25°C | Arabidopsis thaliana | |
| 4.347 | - |
N-acetyl-D-glucosamine | at pH 7.5 and 25°C | Arabidopsis thaliana | |
| 6.69 | - |
D-glucose | at pH 7.5 and 25°C | Arabidopsis thaliana | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.12 | - |
D-glucose | at pH 7.5 and 25°C | Arabidopsis thaliana | |
| 0.6 | - |
N-acetyl-D-galactosamine | at pH 7.5 and 25°C | Arabidopsis thaliana | |
| 10.49 | - |
ATP | at pH 7.5 and 25°C | Arabidopsis thaliana | |
| 94.77 | - |
N-acetyl-D-glucosamine | at pH 7.5 and 25°C | Arabidopsis thaliana | |
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Release 2023.1 (January 2023)
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