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Literature summary for 2.7.1.59 extracted from

  • Barkulis, S.S.
    N-Acetyl-D-glucosamine kinase. I. Steptococcus pyrogenes (1966), Methods Enzymol., 9, 415-420.
No PubMed abstract available

Activating Compound

Activating Compound Comment Organism Structure
cysteine reverses inhibition with p-hydroxymercuribenzoate Streptococcus pyogenes

General Stability

General Stability Organism
ammonium sulfate partially stabilizes against temperature inactivation Streptococcus pyogenes
dialysis at 4°C for 17 h against 0.005 M Tris buffer, pH 7.6, 92% loss of activity, 33% loss of activity in presence of 0.1 M N-acetyl-D-glucosamine or glucose, stable in presence of 0.2 M or 2.5 Streptococcus pyogenes
substrate at 0.2 M stabilizes, unstable in absence of substrate at 4°C Streptococcus pyogenes

Inhibitors

Inhibitors Comment Organism Structure
D-glucose competitive with N-acetyl-D-glucosamine Streptococcus pyogenes
N-acetyl-D-glucosamine competitive with glucose Streptococcus pyogenes
p-hydroxymercuribenzoate no protection by substrate, protection but not reversal of inhibition by thioethanol, complete inhibition above 0.00125 mM, cysteine reverses inhibition Streptococcus pyogenes

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00077
-
N-acetyl-D-glucosamine 30°C, pH 7.4 Streptococcus pyogenes
0.0011
-
D-glucose 30°C, pH 7.4 Streptococcus pyogenes

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + N-acetyl-D-glucosamine Streptococcus pyogenes
-
ADP + N-acetyl-D-glucosamine 6-phosphate
-
?
ATP + N-acetyl-D-glucosamine Streptococcus pyogenes 523
-
ADP + N-acetyl-D-glucosamine 6-phosphate
-
?

Organism

Organism UniProt Comment Textmining
Streptococcus pyogenes
-
serotype 14
-
Streptococcus pyogenes 523
-
serotype 14
-

Purification (Commentary)

Purification (Comment) Organism
-
Streptococcus pyogenes

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
250
-
purified enzyme, 30°C, pH 7.4 Streptococcus pyogenes

Storage Stability

Storage Stability Organism
-25°C, stable for several months Streptococcus pyogenes

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + D-glucose at equal rate than N-acetyl-D-glucosamine Streptococcus pyogenes ADP + D-glucose 6-phosphate
-
?
ATP + D-glucose at equal rate than N-acetyl-D-glucosamine Streptococcus pyogenes 523 ADP + D-glucose 6-phosphate
-
?
ATP + N-acetyl-D-glucosamine
-
Streptococcus pyogenes ADP + N-acetyl-D-glucosamine 6-phosphate
-
?
ATP + N-acetyl-D-glucosamine
-
Streptococcus pyogenes 523 ADP + N-acetyl-D-glucosamine 6-phosphate
-
?
additional information D-fructose, D-mannose, D-maltose, D-galactose, N-acetyl-D-galactosamine, N-acetyl-D-mannosamine do not serve as substrates Streptococcus pyogenes ?
-
?
additional information D-fructose, D-mannose, D-maltose, D-galactose, N-acetyl-D-galactosamine, N-acetyl-D-mannosamine do not serve as substrates Streptococcus pyogenes 523 ?
-
?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
25% loss of activity within 1 min in presence of 13% ammonium sulfate, stable for 1 h in presence of ammonium sulfate and substrate Streptococcus pyogenes
70
-
complete inactivation with 1 min Streptococcus pyogenes

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 8 strongly decreasing activity outside this range Streptococcus pyogenes

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0009
-
N-acetyl-D-glucosamine for reaction with glucose, 30°C, pH 7.4 Streptococcus pyogenes
0.0018
-
D-glucose for reaction with N-acetyl-D-glucosamine, 30°C, pH 7.4 Streptococcus pyogenes