Literature summary for 2.7.1.50 extracted from

Jeyakanthan, J.; Thamotharan, S.; Velmurugan, D.; Rao, V.S.; Nagarajan, S.; Shinkai, A.; Kuramitsu, S.; Yokoyama, S.
New structural insights and molecular-modelling studies of 4-methyl-5-beta-hydroxyethylthiazole kinase from Pyrococcus horikoshii OT3 (PhThiK) (2009), Acta Crystallogr. Sect. F, 65, 978-986.

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Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21-CodonPlus(DE3)-RIL	Pyrococcus horikoshii OT3

Crystallization (Commentary)

Crystallization (Comment)	Organism
precipitant: sodium citrate, pH 9.3, 293 K	Pyrococcus horikoshii OT3

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	amino-acid residues in the magnesium ion-binding site are conserved	Pyrococcus horikoshii OT3

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
28950	-	3 * 28950 Da, crystal structure, calculated	Pyrococcus horikoshii OT3

Organism

Organism	UniProt	Comment	Textmining
Pyrococcus horikoshii OT3	O58877	-	-

Purification (Commentary)

Purification (Comment)	Organism
heat treatment (363 K, 13 min), centrifugation, ion exchange chromatography, hydroxyapatite chromatography, gel filtration,	Pyrococcus horikoshii OT3

Subunits

Subunits	Comment	Organism
homotrimer	3 * 28950 Da, crystal structure, calculated	Pyrococcus horikoshii OT3

Synonyms

Synonyms	Comment	Organism
4-methyl-5-beta-hydroxyethylthiazole kinase	-	Pyrococcus horikoshii OT3

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Release 2023.1 (January 2023)