Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.7.1.43 extracted from

  • Gangl, R.; Behmueller, R.; Tenhaken, R.
    Molecular cloning of a novel glucuronokinase/putative pyrophosphorylase from zebrafish acting in an UDP-glucuronic acid salvage pathway (2014), PLoS ONE, 9, e89690.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells and Nicotiana benthamiana leaves Danio rerio

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.031
-
D-glucuronate at pH 7.5 and 35°C Danio rerio
0.059
-
ATP at pH 7.5 and 35°C Danio rerio

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ Co2+ is able to substitute nfor Mg2+ Danio rerio
Mg2+ required Danio rerio
Mn2+ Mn2+ is able to substitute nfor Mg2+ Danio rerio

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
70000
-
x * 70000, SDS-PAGE Danio rerio

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + D-glucuronate Danio rerio
-
ADP + 1-phospho-alpha-D-glucuronate
-
?

Organism

Organism UniProt Comment Textmining
Danio rerio
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Protino-Ni1000 column chromatography and Strep-Tactin Macro Prep resin column chromatography Danio rerio

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + D-glucuronate
-
Danio rerio ADP + 1-phospho-alpha-D-glucuronate
-
?
additional information no activity with CTP, GTP, TTP, dATP, dCTP, dGTP and dTTP, D-glucose, D-xylose, L-arabinose, L-fucose, D-mannose, L-rhamnose, D-galactose, and D-galacturonate Danio rerio ?
-
?

Subunits

Subunits Comment Organism
? x * 70000, SDS-PAGE Danio rerio

Synonyms

Synonyms Comment Organism
GKUP bifunctional enzyme glucuronokinase and putative uridyl pyrophosphorylase activities Danio rerio
glucuronokinase
-
Danio rerio

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35 40 temperatures above 45°C lead to rapid inactivation of the enzyme Danio rerio

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5 8 no activity is measured below pH 4.5, whereas the maximum is reached between pH 7.5 and 8 with a far lower activity above pH 8.5 Danio rerio