Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells and Nicotiana benthamiana leaves | Danio rerio |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.031 | - |
D-glucuronate | at pH 7.5 and 35°C | Danio rerio | |
0.059 | - |
ATP | at pH 7.5 and 35°C | Danio rerio |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | Co2+ is able to substitute nfor Mg2+ | Danio rerio | |
Mg2+ | required | Danio rerio | |
Mn2+ | Mn2+ is able to substitute nfor Mg2+ | Danio rerio |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
70000 | - |
x * 70000, SDS-PAGE | Danio rerio |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + D-glucuronate | Danio rerio | - |
ADP + 1-phospho-alpha-D-glucuronate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Danio rerio | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Protino-Ni1000 column chromatography and Strep-Tactin Macro Prep resin column chromatography | Danio rerio |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + D-glucuronate | - |
Danio rerio | ADP + 1-phospho-alpha-D-glucuronate | - |
? | |
additional information | no activity with CTP, GTP, TTP, dATP, dCTP, dGTP and dTTP, D-glucose, D-xylose, L-arabinose, L-fucose, D-mannose, L-rhamnose, D-galactose, and D-galacturonate | Danio rerio | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 70000, SDS-PAGE | Danio rerio |
Synonyms | Comment | Organism |
---|---|---|
GKUP | bifunctional enzyme glucuronokinase and putative uridyl pyrophosphorylase activities | Danio rerio |
glucuronokinase | - |
Danio rerio |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | 40 | temperatures above 45°C lead to rapid inactivation of the enzyme | Danio rerio |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | 8 | no activity is measured below pH 4.5, whereas the maximum is reached between pH 7.5 and 8 with a far lower activity above pH 8.5 | Danio rerio |