Protein Variants | Comment | Organism |
---|---|---|
additional information | generation of W3110 derivative strains VH33, VH34 and VH35, that lack the main phosphoenolpyruvate consumers phosphoenolpyruvate:sugar phosphotransferase system and pyruvate kinase isozymes PykA and PykF causing modifications on cell physiology, carbon flux distribution and aromatics production capacity, overview. The phosphoenolpyruvate:sugar phosphotransferase-deficient strain shows lower specific rates for growth, glucose consumption and acetate production as well as a higher biomass yield from glucose. The effects are even more pronounced by the additional inactivation of PykA or PykF. The wild-type and mutant strains are modified to overproduce L-phenylalanine. In resting cells experiments, compared to reference strain, a 10, 4 and 7fold higher aromatics yields from glucose are observed as consequence of PTS, PTS PykA and PTS PykF inactivation, overview | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + pyruvate | Escherichia coli | - |
ADP + phosphoenolpyruvate | - |
? | |
ATP + pyruvate | Escherichia coli K-12 W3110 | - |
ADP + phosphoenolpyruvate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
isozymes PykA and PykF encoded by genes pykA and pykF | - |
Escherichia coli K-12 W3110 | - |
isozymes PykA and PykF encoded by genes pykA and pykF | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + pyruvate | - |
Escherichia coli | ADP + phosphoenolpyruvate | - |
? | |
ATP + pyruvate | - |
Escherichia coli K-12 W3110 | ADP + phosphoenolpyruvate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PYK | - |
Escherichia coli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | - |
assay at | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Escherichia coli |
General Information | Comment | Organism |
---|---|---|
metabolism | phosphoenolpyruvate enters the aromatic amino acids biosynthesis, metabolic flux distribution and Pyk activity in wild-type strain W3110 and in modified strains VH33, VH34 and VH35, overview | Escherichia coli |
physiological function | phosphoenolpyruvate is a key central metabolism intermediate that participates in glucose transport, as precursor in several biosynthetic pathways and it is involved in allosteric regulation of glycolytic enzymes | Escherichia coli |