Cloned (Comment) | Organism |
---|---|
expression profiling by RT-PCR techniques | Brassica juncea |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
3-phosphoglycerate | 8% inhibition at 5 mM | Brassica juncea | |
AMP | 11% inhibition at 5 mM | Brassica juncea | |
ATP | 48% inhibition at 5 mM, significant allosteric effector of the partially purified enzyme | Brassica juncea | |
citrate | 60% inhibition at 5 mM, significant allosteric effector of the partially purified enzyme | Brassica juncea | |
isocitrate | 30% inhibition at 5 mM | Brassica juncea | |
additional information | poor inhibition of glucose 1-phosphate and fumaric acid at 5 mM | Brassica juncea |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics, in presence of saturated substrate concentration, the enzyme exhibits hyperbolic kinetics for both ADP and PEP | Brassica juncea | |
0.0965 | - |
ADP | pH 7.0, 25°C | Brassica juncea | |
0.1785 | - |
phosphoenolpyruvate | pH 7.0, 25°C | Brassica juncea |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast | plastidial isozyme | Brassica juncea | 9507 | - |
cytoplasm | cytosolic isozyme | Brassica juncea | 5737 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Brassica juncea |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + pyruvate | Brassica juncea | - |
ADP + phosphoenolpyruvate | - |
r | |
ATP + pyruvate | Brassica juncea Pusa Bold | - |
ADP + phosphoenolpyruvate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Brassica juncea | - |
- |
- |
Brassica juncea Pusa Bold | - |
- |
- |
Purification (Comment) | Organism |
---|---|
native cytosolic isozyme 12.3fold from seeds by anion exchange and hydroxyapatite chromatography, inactivation of plastidial isoform by heating the enzyme extract at 60°C for 3 min | Brassica juncea |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
flower | - |
Brassica juncea | - |
leaf | - |
Brassica juncea | - |
seed | expression profiling of cytosolic pyruvate kinase enzyme during seed development, enzyme activity is highest during middle stage, overview | Brassica juncea | - |
seedling | highest activity | Brassica juncea | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
690.7 | - |
partially purified cytosolic isozyme, pH 7.0, 25°C | Brassica juncea |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + pyruvate | - |
Brassica juncea | ADP + phosphoenolpyruvate | - |
r | |
ATP + pyruvate | - |
Brassica juncea Pusa Bold | ADP + phosphoenolpyruvate | - |
r |
Synonyms | Comment | Organism |
---|---|---|
PYK | - |
Brassica juncea |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Brassica juncea |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
3 min, pH 7.0, plastidial isoform, inactivation | Brassica juncea |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
narrow optimum, cytosolic isozyme | Brassica juncea |
8 | - |
plastidial isozyme | Brassica juncea |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Brassica juncea |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
3.64 | - |
pH 7.0, 25°C | Brassica juncea | citrate | |
5 | - |
pH 7.0, 25°C | Brassica juncea | ATP |
General Information | Comment | Organism |
---|---|---|
metabolism | pyruvate kinase is a crucial regulatory enzyme involved in glycolysis | Brassica juncea |