Any feedback?
Literature summary for 2.7.1.40 extracted from
- Effector analogues detect varied allosteric roles for conserved protein-effector interactions in pyruvate kinase isozymes (2011), Biochemistry, 50, 1934-1939.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of wild-type and mutant H476L in the FF50 strain of Escherichia coli | Homo sapiens |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| H476L | site-directed mutagenesis | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2-aminoisobutyric acid | allosteric inhibition | Homo sapiens |  |
| D-alanine | allosteric inhibition | Homo sapiens | |
| L-alanine | allosteric inhibition. The pyruvate kinase isozyme from human liver has decreased affinity for phosphoenolpyruvate when allosterically inhibited by alanine. Minimal effect on coupling caused by the methyl group substitution to Ala (2-aminoisobutyric acid vs. Ala) | Homo sapiens | |
| L-cysteine | allosteric inhibition | Homo sapiens | |
| L-phenylalanine | allosteric inhibition. Replacement of the alpha-hydrogen of L-Phe with a methyl group (S)-2-amino-2-methyl-3-phenyl-propionic acid eliminates an allosteric response | Homo sapiens | |
| L-proline | allosteric inhibition | Homo sapiens | |
| L-valine | allosteric inhibition | Homo sapiens | |
| additional information | no inhibition by D-phenylalanine and (S)-2-amino-2-methyl-3-phenyl-propionic acid. Analysis of the binding site for allosteric inhibitor amino acids and the allosteric kinetic mechanism, overview. L-Phe elicits the smallest antagonism of phosphoenolpyruvate affinity of all amino acids tested | Homo sapiens |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required | Homo sapiens | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + pyruvate | Homo sapiens | - |
ADP + phosphoenolpyruvate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant wild-type and mutant H476L from the FF50 strain of Escherichia coli by ammonium sulfate fractionation and anion exchange chromatography | Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | liver-specific isozyme hL-PYK | Homo sapiens | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + pyruvate | - |
Homo sapiens | ADP + phosphoenolpyruvate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| hL-PYK | - |
Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Homo sapiens |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Homo sapiens | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
