Activating Compound | Comment | Organism | Structure |
---|---|---|---|
cysteine | activation, can replace dithiothreitol | Trypanosoma brucei | |
D-fructose 1,6-diphosphate | activation | Trypanosoma brucei | |
D-fructose 1,6-diphosphate | kinetics | Trypanosoma brucei | |
D-glucose 6-phosphate | - |
Trypanosoma brucei | |
dithiothreitol | activation | Trypanosoma brucei | |
GSH | activation, can replace dithiothreittol | Trypanosoma brucei |
General Stability | Organism |
---|---|
glycerol stabilizes during purification | Trypanosoma brucei |
unstable in aqueous solution, thiol protecting agents and glycerol stabilize | Trypanosoma brucei |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
K+ | above 100 mM, activates below | Trypanosoma brucei | |
Na+ | above 100 mM, activates below | Trypanosoma brucei | |
NH4+ | above 100 mM, activates below | Trypanosoma brucei |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.082 | 0.4 | ADP | pH 7.4, 25°C | Trypanosoma brucei | |
0.098 | 0.1 | GDP | pH 7.4, 25°C | Trypanosoma brucei | |
0.41 | - |
UDP | pH 7.4, 25°C | Trypanosoma brucei | |
2.53 | - |
CDP | pH 7.4, 25°C | Trypanosoma brucei |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
K+ | requirement | Trypanosoma brucei | |
K+ | inhibits above 100 mM | Trypanosoma brucei | |
Mg2+ | requirement | Trypanosoma brucei | |
Mn2+ | requirement | Trypanosoma brucei | |
monovalent cation | requirement | Trypanosoma brucei | |
Na+ | inhibits above 100 mM | Trypanosoma brucei | |
NH4+ | requirement | Trypanosoma brucei | |
NH4+ | can replace K+ | Trypanosoma brucei | |
NH4+ | inhibits above 100 mM | Trypanosoma brucei |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
270000 | - |
gel filtration | Trypanosoma brucei |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trypanosoma brucei | - |
- |
- |
Purification (Comment) | Organism |
---|---|
partial | Trypanosoma brucei |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell culture | - |
Trypanosoma brucei | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Trypanosoma brucei |
Storage Stability | Organism |
---|---|
4°C, lyophilized lyzed trypanosomes, 3 h after resuspension, 20% loss of activity, storage in aqueous solution leads to a decrease in activity and cooperative nature of phosphoenolpyruvate-enzyme interaction | Trypanosoma brucei |
glycerol, 25% w/v, or 0.1 mM dithiothreitol stabilizes, more effective under refrigerated conditions than at room temperature, KCl, MgSO4, EDTA, phosphoenolpyruvate, ADP or (NH4)2SO4 decreases stability at either 4°C or 17°C | Trypanosoma brucei |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ADP + phosphoenolpyruvate | best nucleoside diphosphate substrate | Trypanosoma brucei | ATP + pyruvate | - |
? | |
ADP + phosphoenolpyruvate | positive cooperativity for phosphoenolpyruvate | Trypanosoma brucei | ATP + pyruvate | - |
? | |
CDP + phosphoenolpyruvate | less effective than ADP | Trypanosoma brucei | CTP + pyruvate | - |
? | |
GDP + phosphoenolpyruvate | less effective than ADP | Trypanosoma brucei | GTP + pyruvate | - |
? | |
additional information | preferred: purine nucleotides | Trypanosoma brucei | ? | - |
? | |
UDP + phosphoenolpyruvate | less effective than ADP | Trypanosoma brucei | UTP + pyruvate | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Trypanosoma brucei |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | 7 | - |
Trypanosoma brucei |