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Literature summary for 2.7.1.40 extracted from

  • Lin, M.; Turpin, D.H.; Plaxton, W.C.
    Pyruvate kinase isozymes from the green alga, Selenastrum minutum. I. Purification and physical and immunological characterization (1989), Arch. Biochem. Biophys., 269, 219-227.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
N-ethylmaleimide isozyme PK1 is more sensitive than PK2 Monoraphidium minutum

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol 2 isozymes: a cytosolic and a plastid one Monoraphidium minutum 5829
-

Metals/Ions

Metals/Ions Comment Organism Structure
K+
-
Monoraphidium minutum
Mg2+
-
Monoraphidium minutum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
57000
-
10 * 57000, isozyme PK2, SDS-PAGE Monoraphidium minutum
240000
-
isozyme PK1 Monoraphidium minutum
590000
-
isozyme PK1 Monoraphidium minutum

Organism

Organism UniProt Comment Textmining
Monoraphidium minutum
-
green alga
-

Purification (Commentary)

Purification (Comment) Organism
2 immunologically unrelated isoforms, PK1 and 2, separable by anion exchange chromatography Monoraphidium minutum
partial Monoraphidium minutum

Source Tissue

Source Tissue Comment Organism Textmining
cell culture
-
Monoraphidium minutum
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Monoraphidium minutum

Storage Stability

Storage Stability Organism
-80°C, frozen with liquid N2, several months Monoraphidium minutum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ADP + phosphoenolpyruvate
-
Monoraphidium minutum ATP + pyruvate
-
ir

Subunits

Subunits Comment Organism
decamer 10 * 57000, isozyme PK2, SDS-PAGE Monoraphidium minutum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Monoraphidium minutum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
3 min, isozyme PK1: 34% loss of activity, isozyme PK2: stable Monoraphidium minutum
60
-
3 min, 26% loss of activity, isozyme PK2 Monoraphidium minutum