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Literature summary for 2.7.1.40 extracted from

  • Baysdorfer, C.; Bassham, J.A.
    Spinach pyruvate kinase isoforms. Partial purification and regulatory properties (1984), Plant Physiol., 74, 374-379.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
asparagine activation Spinacia oleracea
asparagine synergistic activation with fructose 1,6-diphosphate of isozyme PK-aerobic, not PK-anoxic Spinacia oleracea
D-fructose 1,6-diphosphate not Spinacia oleracea
D-fructose 2,6-diphosphate not Spinacia oleracea

Inhibitors

Inhibitors Comment Organism Structure
2-oxoglutarate not Spinacia oleracea
adenine weak Spinacia oleracea
citrate isozyme PKI, kinetics; not isozyme PKII Spinacia oleracea
L-glutamate not isozyme PKI; strong, isozyme PKII, kinetics Spinacia oleracea
oxalate kinetics Spinacia oleracea
tryptophan weak Spinacia oleracea

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information kinetic parameters Saccharomyces cerevisiae
additional information
-
additional information kinetic parameters Spinacia oleracea
0.72 0.73 UDP pH 7.1, 25°C Spinacia oleracea
1.1
-
GDP pH 7.1, 25°C Spinacia oleracea
6.8
-
CDP isozyme PKII, pH 7.1, 25°C Spinacia oleracea
9
-
CDP isozyme PKI, pH 7.1, 25°C Spinacia oleracea

Metals/Ions

Metals/Ions Comment Organism Structure
K+
-
Saccharomyces cerevisiae
K+
-
Spinacia oleracea
Mg2+
-
Saccharomyces cerevisiae
Mg2+
-
Spinacia oleracea

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-
Spinacia oleracea
-
spinach
-

Purification (Commentary)

Purification (Comment) Organism
partial, 2 isoforms, separable by blue-agarose chromatography Spinacia oleracea

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
Spinacia oleracea
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Saccharomyces cerevisiae
additional information
-
-
Spinacia oleracea

Storage Stability

Storage Stability Organism
-20°C, in the absence of substrates, 70% loss of activity within 1 month, phosphoenolpyruvate reduces this loss somewhat, ADP increases it Spinacia oleracea
-80°C, in the absence of substrates, 95% loss of activity within 1 month, phosphoenolpyruvate reduces this loss somewhat, ADP increases it Spinacia oleracea
4°C, in the absence of substrates, t1/2: 1 month Spinacia oleracea
8°C, isozyme PKI in the absence of substrates, less than 20% loss of activity within 1 month and isozyme PKII with t1/2 of 5 days, 25% loss within 1 month in the presence of phosphoenolpyruvate or 60% loss of activity in the presence of ADP Spinacia oleracea

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ADP + phosphoenolpyruvate
-
Saccharomyces cerevisiae ATP + pyruvate
-
?
ADP + phosphoenolpyruvate best nucleoside diphosphate substrate Spinacia oleracea ATP + pyruvate
-
?
CDP + phosphoenolpyruvate less effective than ADP Spinacia oleracea CTP + pyruvate
-
?
GDP + phosphoenolpyruvate less effective than ADP Spinacia oleracea GTP + pyruvate
-
?
UDP + phosphoenolpyruvate less effective than ADP Spinacia oleracea UTP + pyruvate
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Spinacia oleracea

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
cold-labile enzyme Saccharomyces cerevisiae
additional information
-
cold-labile enzyme Spinacia oleracea

pH Range

pH Minimum pH Maximum Comment Organism
5.5 7.5 about 80% of maximal activity at pH 5.5 and pH 7.5 Spinacia oleracea

Cofactor

Cofactor Comment Organism Structure
AMP activation Spinacia oleracea
AMP not at physiological concentrations Spinacia oleracea