Activating Compound | Comment | Organism | Structure |
---|---|---|---|
asparagine | activation | Spinacia oleracea | |
asparagine | synergistic activation with fructose 1,6-diphosphate of isozyme PK-aerobic, not PK-anoxic | Spinacia oleracea | |
D-fructose 1,6-diphosphate | not | Spinacia oleracea | |
D-fructose 2,6-diphosphate | not | Spinacia oleracea |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-oxoglutarate | not | Spinacia oleracea | |
adenine | weak | Spinacia oleracea | |
citrate | isozyme PKI, kinetics; not isozyme PKII | Spinacia oleracea | |
L-glutamate | not isozyme PKI; strong, isozyme PKII, kinetics | Spinacia oleracea | |
oxalate | kinetics | Spinacia oleracea | |
tryptophan | weak | Spinacia oleracea |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetic parameters | Saccharomyces cerevisiae | |
additional information | - |
additional information | kinetic parameters | Spinacia oleracea | |
0.72 | 0.73 | UDP | pH 7.1, 25°C | Spinacia oleracea | |
1.1 | - |
GDP | pH 7.1, 25°C | Spinacia oleracea | |
6.8 | - |
CDP | isozyme PKII, pH 7.1, 25°C | Spinacia oleracea | |
9 | - |
CDP | isozyme PKI, pH 7.1, 25°C | Spinacia oleracea |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
K+ | - |
Saccharomyces cerevisiae | |
K+ | - |
Spinacia oleracea | |
Mg2+ | - |
Saccharomyces cerevisiae | |
Mg2+ | - |
Spinacia oleracea |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Spinacia oleracea | - |
spinach | - |
Purification (Comment) | Organism |
---|---|
partial, 2 isoforms, separable by blue-agarose chromatography | Spinacia oleracea |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Spinacia oleracea | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Saccharomyces cerevisiae |
additional information | - |
- |
Spinacia oleracea |
Storage Stability | Organism |
---|---|
-20°C, in the absence of substrates, 70% loss of activity within 1 month, phosphoenolpyruvate reduces this loss somewhat, ADP increases it | Spinacia oleracea |
-80°C, in the absence of substrates, 95% loss of activity within 1 month, phosphoenolpyruvate reduces this loss somewhat, ADP increases it | Spinacia oleracea |
4°C, in the absence of substrates, t1/2: 1 month | Spinacia oleracea |
8°C, isozyme PKI in the absence of substrates, less than 20% loss of activity within 1 month and isozyme PKII with t1/2 of 5 days, 25% loss within 1 month in the presence of phosphoenolpyruvate or 60% loss of activity in the presence of ADP | Spinacia oleracea |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ADP + phosphoenolpyruvate | - |
Saccharomyces cerevisiae | ATP + pyruvate | - |
? | |
ADP + phosphoenolpyruvate | best nucleoside diphosphate substrate | Spinacia oleracea | ATP + pyruvate | - |
? | |
CDP + phosphoenolpyruvate | less effective than ADP | Spinacia oleracea | CTP + pyruvate | - |
? | |
GDP + phosphoenolpyruvate | less effective than ADP | Spinacia oleracea | GTP + pyruvate | - |
? | |
UDP + phosphoenolpyruvate | less effective than ADP | Spinacia oleracea | UTP + pyruvate | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Spinacia oleracea |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
cold-labile enzyme | Saccharomyces cerevisiae |
additional information | - |
cold-labile enzyme | Spinacia oleracea |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.5 | 7.5 | about 80% of maximal activity at pH 5.5 and pH 7.5 | Spinacia oleracea |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
AMP | activation | Spinacia oleracea | |
AMP | not at physiological concentrations | Spinacia oleracea |