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Literature summary for 2.7.1.40 extracted from

  • Plaxton, W.C.; Storey, K.B.
    Purification and properties of aerobic and anoxic forms of pyruvate kinase from red muscle tissue of the channelled whelk, Busycotypus canaliculatum (1984), Eur. J. Biochem., 143, 257-265.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
D-fructose 1,6-diphosphate activation Busycotypus canaliculatum
D-fructose 2,6-diphosphate activation Busycotypus canaliculatum

Inhibitors

Inhibitors Comment Organism Structure
2-oxoglutarate not Busycotypus canaliculatum
3-phosphoglycerate not Busycotypus canaliculatum
ADP at high concentrations; fructose 1,6-diphosphate partially restores Busycotypus canaliculatum
AMP MgAMP Busycotypus canaliculatum
arginine phosphate allosteric inhibitor; fructose 1,6-diphosphate restores Busycotypus canaliculatum
ATP MgATP2- Busycotypus canaliculatum
citrate not Busycotypus canaliculatum
glucose 6-phosphate not Busycotypus canaliculatum
isocitrate not Busycotypus canaliculatum
L-alanine allosteric inhibitor; fructose 1,6-diphosphate restores Busycotypus canaliculatum
L-lactate
-
Busycotypus canaliculatum
L-phenylalanine alanine and fructose 1,6-diphosphate protect, kinetics; allosteric inhibitor; pH-dependent Busycotypus canaliculatum
malate not Busycotypus canaliculatum
MgATP2- allosteric inhibitor; fructose 1,6-diphosphate restores Busycotypus canaliculatum
additional information no inhibition by acetyl-CoA, NADP+, succinate, glycerol 1-phosphate, fructose 6-phosphate, D-octopine, meso-alanopine, NH4Cl, Arg, Gly, taurine, creatine phosphate Busycotypus canaliculatum
oxaloacetate not Busycotypus canaliculatum
phosphate not Busycotypus canaliculatum
proline allosteric inhibitor, fructose 1,6-diphosphate restores Busycotypus canaliculatum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information kinetic properties Busycotypus canaliculatum
0.2 0.35 ADP pH 7.0, 20°C Busycotypus canaliculatum

Metals/Ions

Metals/Ions Comment Organism Structure
K+
-
Busycotypus canaliculatum
Mg2+
-
Busycotypus canaliculatum
Mn2+ can partially replace Mg2+ Busycotypus canaliculatum
NH4+ requirement Busycotypus canaliculatum
NH4+ can replace K+ Busycotypus canaliculatum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
64400
-
4 * 64400, PK-aerobic, SDS-PAGE Busycotypus canaliculatum
232600
-
PK-anoxic, gel filtration Busycotypus canaliculatum
237500
-
PK-aerobic, gel filtration Busycotypus canaliculatum

Organism

Organism UniProt Comment Textmining
Busycotypus canaliculatum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
aerobic and anoxic variants, i.e. isozymes PK-aerobic and PK-anoxic Busycotypus canaliculatum

Source Tissue

Source Tissue Comment Organism Textmining
muscle radular retractor muscle Busycotypus canaliculatum
-
muscle red muscle Busycotypus canaliculatum
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Busycotypus canaliculatum
368
-
isozyme PK-aerobic, pH 7.0, 20°C Busycotypus canaliculatum

Storage Stability

Storage Stability Organism
4°C, in 25 mM imidazole-HCl buffer, pH 7.1, 10 mM 2-mercaptoethanol, 1 mM EDTA, 0.5 M sucrose and 0.04% NaN3, concentrated by dialysis against solid polyethylene glycol, at least 4 weeks, isozyme PK-aerobic Busycotypus canaliculatum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ADP + phosphoenolpyruvate
-
Busycotypus canaliculatum ATP + pyruvate
-
?

Subunits

Subunits Comment Organism
tetramer 4 * 64400, PK-aerobic, SDS-PAGE Busycotypus canaliculatum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
additional information
-
pH-dependence of phosphoenolpyruvate affinity Busycotypus canaliculatum
7
-
broad Busycotypus canaliculatum

pH Range

pH Minimum pH Maximum Comment Organism
6 8 about 70% of maximal activity at pH 6 and about half-maximal activity at pH 8 Busycotypus canaliculatum

pI Value

Organism Comment pI Value Maximum pI Value
Busycotypus canaliculatum isozyme PK-anoxic, isoelectric focusing
-
5.42
Busycotypus canaliculatum isozyme PK-aerobic, isoelectric focusing
-
5.81