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Literature summary for 2.7.1.40 extracted from
- Purification and properties of pyruvate kinase type M2 from rat lung (1982), Biochim. Biophys. Acta, 717, 337-347.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| D-fructose 1,6-diphosphate | activation | Rattus norvegicus |  |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | - |
Rattus norvegicus | |
| cysteine | fructose 1,6-diphosphate protects | Rattus norvegicus | |
| D-serine | - |
Rattus norvegicus | |
| L-alanine | fructose 1,6-diphosphate restores | Rattus norvegicus | |
| L-phenylalanine | fructose 1,6-diphosphate protects | Rattus norvegicus | |
| additional information | no phosphorylation | Rattus norvegicus | |
| threonine | fructose 1,6-diphosphate protects | Rattus norvegicus | |
| tyrosine | fructose 1,6-diphosphate partially protects | Rattus norvegicus | |
| valine | fructose 1,6-diphosphate protects | Rattus norvegicus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | kinetic parameters | Rattus norvegicus | |
| 0.082 | 0.4 | ADP | pH 7.1, 37°C | Rattus norvegicus | |
| 0.67 | - |
GDP | pH 7.1, 37°C | Rattus norvegicus | |
| 1.25 | - |
UDP | pH 7.1, 37°C | Rattus norvegicus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| K+ | - |
Rattus norvegicus | |
| Mg2+ | - |
Rattus norvegicus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 224000 | - |
gel filtration | Rattus norvegicus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| lung | M2-type isozyme | Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1341 | - |
pH 7.1, 37°C | Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ADP + phosphoenolpyruvate | best nucleoside diphosphate substrate | Rattus norvegicus | ATP + pyruvate | - |
? | |
| CDP + phosphoenolpyruvate | less effective than ADP | Rattus norvegicus | CTP + pyruvate | - |
? | |
| GDP + phosphoenolpyruvate | less effective than ADP | Rattus norvegicus | GTP + pyruvate | - |
? | |
| IDP + phosphoenolpyruvate | less effective than ADP | Rattus norvegicus | ITP + pyruvate | - |
? | |
| UDP + phosphoenolpyruvate | less effective than ADP | Rattus norvegicus | UTP + pyruvate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 62000-66000, SDS-PAGE | Rattus norvegicus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Rattus norvegicus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
- |
Rattus norvegicus |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Rattus norvegicus | - |
- |
5.8 |
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