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Literature summary for 2.7.1.40 extracted from

  • Imamura, K.; Tanaka, T.
    Pyruvate kinase isozymes from rat (1982), Methods Enzymol., 90, 150-165.
No PubMed abstract available
Search for more literature for 2.7.1.40

Activating Compound

Activating Compound Comment Organism Structure
Insulin stimulation Rattus norvegicus

Crystallization (Commentary)

Crystallization (Comment) Organism
liver L-type isozyme, erythrocytic R-type isozyme, M1- and M2-type isozymes Rattus norvegicus

General Stability

General Stability Organism
fructose 1,6-diphosphate and bovine serum albumin stabilize Rattus norvegicus
phosphate buffer, MgSO4, 2-mercaptoethanol, fructose 1,6-diphosphate stabilize during purification at room temperature Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
ATP L- and R-type isozyme; phosphorylated enzyme is more sensitive than unphosphorylated enzyme Rattus norvegicus
catecholamine
-
 Rattus norvegicus
glucagon
-
 Rattus norvegicus
L-alanine L- and M2-type, not M1-type isozyme Rattus norvegicus
L-phenylalanine L- and M2-type, not M1-type isozyme Rattus norvegicus
additional information phosphorylation by cAMP-dependent protein kinase, L- and R-type isozyme Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.049 0.1 IDP cosubstrate phosphoenolpyruvate Rattus norvegicus
0.082 0.4 ADP L-type isozyme, pH 7.4, 37°C Rattus norvegicus
0.3 0.96 phosphoenolpyruvate L-type, pH 7.4, 37°C Rattus norvegicus
0.4 0.6 ADP R-type isozyme, pH 7.4, 37°C Rattus norvegicus
0.4 0.57 phosphoenolpyruvate M2-type isozyme, pH 7.4, 37°C Rattus norvegicus
0.98 1 ADP M1-type isozyme, pH 7.4, 37°C Rattus norvegicus
1.4
-
 phosphoenolpyruvate R-type isozyme, pH 7.4, 37°C Rattus norvegicus
1.4 1.5 ADP M2-type isozyme, pH 7.4, 37°C Rattus norvegicus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
 Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
multiple isozymes Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
brain M1-type isozyme Rattus norvegicus
-
erythrocyte R-type isozyme Rattus norvegicus
-
fetus M2-type isozyme Rattus norvegicus
-
heart type M-isozyme Rattus norvegicus
-
hematopoietic system R-type isozyme Rattus norvegicus
-
liver L-type: major, M2-type: minor isozyme Rattus norvegicus
-
additional information tissue distribution Rattus norvegicus
-
muscle skeletal Rattus norvegicus
-
muscle type M-isozyme Rattus norvegicus
-
Yoshida AH-130 cell M2-type isozyme Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
307
-
 pH 7.4, 37°C, R-type isozyme Rattus norvegicus
380
-
 M1-type isozyme, pH 7.4, 37°C Rattus norvegicus
520
-
 M2-type isozyme, 25°C, pH 7.4 Rattus norvegicus
770
-
 M2-type isozyme, 37°C, pH 7.4 Rattus norvegicus
780
-
 M1-type isozyme, 37°C, pH 7.4 Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ADP + phosphoenolpyruvate
-
 Rattus norvegicus ATP + pyruvate
-
 ?
IDP + phosphoenolpyruvate
-
 Rattus norvegicus ITP + pyruvate
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Rattus norvegicus