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Literature summary for 2.7.1.40 extracted from

  • Wu, H.B.; Turpin, D.H.
    Purification and characteization of pyruvate kinase from the green alga Chlamydomonas reinhardtii (1992), J. Phycol., 28, 472-481.
No PubMed abstract available
Search for more literature for 2.7.1.40

Activating Compound

Activating Compound Comment Organism Structure
D-Ribulose 1,5-diphosphate activation Chlamydomonas reinhardtii
dihydroxyacetone phosphate activation Chlamydomonas reinhardtii
glyceraldehyde 3-phosphate activation Chlamydomonas reinhardtii
ribulose 5-phosphate activation Chlamydomonas reinhardtii

Inhibitors

Inhibitors Comment Organism Structure
2,3-diphosphoglycerate
-
 Chlamydomonas reinhardtii
ATP
-
 Chlamydomonas reinhardtii
citrate
-
 Chlamydomonas reinhardtii
isocitrate
-
 Chlamydomonas reinhardtii
L-glutamate dihydroxyacetone phosphate reverses Chlamydomonas reinhardtii
malate
-
 Chlamydomonas reinhardtii
N-ethylmaleimide
-
 Chlamydomonas reinhardtii
oxalate
-
 Chlamydomonas reinhardtii
Phosphoglycolate
-
 Chlamydomonas reinhardtii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.15 0.16 phosphoenolpyruvate
-
 Chlamydomonas reinhardtii
0.18
-
 phosphoenolpyruvate cosubstrate ADP Chlamydomonas reinhardtii

Metals/Ions

Metals/Ions Comment Organism Structure
K+ activation Chlamydomonas reinhardtii
K+ strong Chlamydomonas reinhardtii
Mg2+ requirement Chlamydomonas reinhardtii
Mg2+ fulfills absolute requirement for divalent cation Chlamydomonas reinhardtii
Mn2+ requirement Chlamydomonas reinhardtii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
57000
-
 4 * 57000, SDS-PAGE Chlamydomonas reinhardtii
224000
-
 native molecular mass Chlamydomonas reinhardtii

Organism

Organism UniProt Comment Textmining
Chlamydomonas reinhardtii
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial Chlamydomonas reinhardtii

Reaction

Reaction Comment Organism Reaction ID
ATP + pyruvate = ADP + phosphoenolpyruvate mechanism Chlamydomonas reinhardtii
a

Source Tissue

Source Tissue Comment Organism Textmining
cell culture
-
 Chlamydomonas reinhardtii
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Chlamydomonas reinhardtii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ADP + phosphoenolpyruvate best nucleoside diphosphate substrate Chlamydomonas reinhardtii ATP + pyruvate
-
 ?
CDP + phosphoenolpyruvate less effective than ADP Chlamydomonas reinhardtii CTP + pyruvate
-
 ?
CDP + phosphoenolpyruvate reaction at 25% the rate of ADP Chlamydomonas reinhardtii CTP + pyruvate
-
 ?
GDP + phosphoenolpyruvate less effective than ADP Chlamydomonas reinhardtii GTP + pyruvate
-
 ?
GDP + phosphoenolpyruvate reaction at 55% the rate of ADP Chlamydomonas reinhardtii GTP + pyruvate
-
 ?
IDP + phosphoenolpyruvate less effective than ADP Chlamydomonas reinhardtii ITP + pyruvate
-
 ?
IDP + phosphoenolpyruvate reaction at 53% the rate of ADP Chlamydomonas reinhardtii ITP + pyruvate
-
 ?
UDP + phosphoenolpyruvate less effective than ADP Chlamydomonas reinhardtii UTP + pyruvate
-
 ?

Subunits

Subunits Comment Organism
tetramer 4 * 57000, SDS-PAGE Chlamydomonas reinhardtii

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
 heat-stable enzyme Chlamydomonas reinhardtii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.8
-
-
 Chlamydomonas reinhardtii

Cofactor

Cofactor Comment Organism Structure
AMP activation Chlamydomonas reinhardtii