Activating Compound | Comment | Organism | Structure |
---|---|---|---|
UTP | stimulation of ATP utilization | Saccharomyces cerevisiae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
p-chloromercuribenzoate | 0.1 mM, 83% inhibition, completely reversed by reduced glutathione | Saccharomyces cerevisiae |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | can partially replace Mn2+ in activation | Saccharomyces cerevisiae | |
Ca2+ | at 5 mM 10% of the activity with Mn2+ | Saccharomyces cerevisiae | |
Co2+ | can partially replace Mn2+ in activation | Saccharomyces cerevisiae | |
Co2+ | approx. 15% of the activity with Mn2+ at 5 mM | Saccharomyces cerevisiae | |
Fe2+ | can partially replace Mn2+ in activation | Saccharomyces cerevisiae | |
Fe2+ | at 5 mM about 10% of the activity with Mn2+ | Saccharomyces cerevisiae | |
Mg2+ | Mg2+ becomes increasingly active at higher concentrations and at sufficiently high concentrations the most active metal ion | Saccharomyces cerevisiae | |
Mn2+ | divalent cation required for activity | Saccharomyces cerevisiae | |
Mn2+ | higher activation than with Mg2+ at concentrations below approx. 0.008 mM, lower activation than with Mg2+ above | Saccharomyces cerevisiae | |
Zn2+ | can partially replace Mn2+ in activation | Saccharomyces cerevisiae | |
Zn2+ | at 5 mM about 30% of the activity of Mn2+ | Saccharomyces cerevisiae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Purification (Comment) | Organism |
---|---|
protamine sulfate, ammonium sulfate, acid treatment at pH 5.0, ammonium sulfate | Saccharomyces cerevisiae |
Storage Stability | Organism |
---|---|
-10°C, several months, no loss of activity | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + mevalonate | no activity with ADP | Saccharomyces cerevisiae | ADP + phosphomevalonate | - |
? | |
CTP + mevalonate | - |
Saccharomyces cerevisiae | CDP + phosphomevalonate | - |
? | |
GTP + mevalonate | - |
Saccharomyces cerevisiae | GDP + phosphomevalonate | - |
? | |
UTP + mevalonate | - |
Saccharomyces cerevisiae | UDP + phosphomevalonate | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.4 | 6.7 | - |
Saccharomyces cerevisiae |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 9 | more than 50% of maximal activity at pH 5.0 and pH 9.0 | Saccharomyces cerevisiae |