Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.7.1.32 extracted from

  • Chang, C.C.; Few, L.L.; Konrad, M.; See Too, W.C.
    Phosphorylation of human choline kinase beta by protein kinase A: its impact on activity and inhibition (2016), PLoS ONE, 11, e0154702.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.1.32

Protein Variants

Protein Variants Comment Organism
S39D/S40D the choline kinase beta phosphorylation mimic behaves kinetically like the wild type enzyme Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
hemicholinium-3
-
 Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.42
-
 choline phosphorylated choline kinase beta, at pH 7.5 and 30°C Homo sapiens
0.46
-
 choline mutant choline kinase beta S39D/S40D, at pH 7.5 and 30°C Homo sapiens
0.58
-
 ATP mutant choline kinase beta S39D/S40D, at pH 7.5 and 30°C Homo sapiens
0.65
-
 ATP phosphorylated choline kinase beta, at pH 7.5 and 30°C Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45000
-
 x * 45000, SDS-PAGE Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + choline Homo sapiens
-
 ADP + phosphocholine
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens Q9Y259
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
phosphoprotein choline kinase beta is phosphorylated by protein kinase A Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + choline
-
 Homo sapiens ADP + phosphocholine
-
 ?

Subunits

Subunits Comment Organism
? x * 45000, SDS-PAGE Homo sapiens

Synonyms

Synonyms Comment Organism
choline kinase beta
-
 Homo sapiens
CKbeta
-
 Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
45.31
-
 choline mutant choline kinase beta S39D/S40D, at pH 7.5 and 30°C Homo sapiens
48.25
-
 choline phosphorylated choline kinase beta, at pH 7.5 and 30°C Homo sapiens
50.48
-
 ATP mutant choline kinase beta S39D/S40D, at pH 7.5 and 30°C Homo sapiens
54.39
-
 ATP phosphorylated choline kinase beta, at pH 7.5 and 30°C Homo sapiens

General Information

General Information Comment Organism
physiological function choline kinase beta, in concert with choline kinase alpha, and depending on its phosphorylation status, plays a critical role in carcinogenesis Homo sapiens

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
83.69
-
 ATP phosphorylated choline kinase beta, at pH 7.5 and 30°C Homo sapiens
86.24
-
 ATP mutant choline kinase beta S39D/S40D, at pH 7.5 and 30°C Homo sapiens
98.5
-
 choline mutant choline kinase beta S39D/S40D, at pH 7.5 and 30°C Homo sapiens
114.9
-
 choline phosphorylated choline kinase beta, at pH 7.5 and 30°C Homo sapiens