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Literature summary for 2.7.1.32 extracted from

  • Yuan, C.; Kent, C.
    Identification of critical residues of ATP kinase A2 from Caenorhabditis elegans (2004), J. Biol. Chem., 279, 17801-17809.
    View publication on PubMed
Search for more literature for 2.7.1.32

Cloned(Commentary)

Cloned (Comment) Organism
native and mutant enzymes expressed as His-tag fusion protein in Sf9 cells Caenorhabditis elegans

Protein Variants

Protein Variants Comment Organism
C252S slightly increased turnover Caenorhabditis elegans
D255A no activity detectable Caenorhabditis elegans
D255E activity almost completely abolished Caenorhabditis elegans
D255N no activity detectable Caenorhabditis elegans
D301A no activity detectable Caenorhabditis elegans
D301E no activity detectable Caenorhabditis elegans
D301N no activity detectable Caenorhabditis elegans
D313A low expression level Caenorhabditis elegans
E125A reduced activity Caenorhabditis elegans
E125D increased turnover Caenorhabditis elegans
E125Q reduced activity Caenorhabditis elegans
E134A slightly increased turnover Caenorhabditis elegans
E151A slightly increased turnover Caenorhabditis elegans
E303A strongly reduced activity, increased Km for both substrates, strongly increased Km for Mg2+ Caenorhabditis elegans
E303D reduced activity Caenorhabditis elegans
E303Q reduced activity Caenorhabditis elegans
E320A slightly increased turnover Caenorhabditis elegans
H181A low expression level Caenorhabditis elegans
H253A reduced activity Caenorhabditis elegans
N260A activity almost completely abolished, strongly increased Km for Mg2+ Caenorhabditis elegans
N308A reduced activity Caenorhabditis elegans
N308D slightly increased turnover Caenorhabditis elegans
N308Q reduced activity Caenorhabditis elegans
N354A strongly reduced activity, only minor effect on Km for both substrates Caenorhabditis elegans
N87A reduced activity Caenorhabditis elegans
R111A reduced activity Caenorhabditis elegans
R149A reduced activity Caenorhabditis elegans
R149K slightly increased turnover Caenorhabditis elegans
S85T reduced activity Caenorhabditis elegans
S86A strongly reduced activity, increased Km for ATP Caenorhabditis elegans
W387A strongly reduced activity, only minor effect on Km for both substrates Caenorhabditis elegans

Inhibitors

Inhibitors Comment Organism Structure
Ca2+
-
 Caenorhabditis elegans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.4
-
 choline N260A mutant enzyme, pH 10, 37°C Caenorhabditis elegans
0.7
-
 choline R149A mutant enzyme, pH 10, 37°C Caenorhabditis elegans
0.7
-
 choline S85T mutant enzyme, pH 10, 37°C Caenorhabditis elegans
0.8
-
 choline E125D mutant enzyme, pH 10, 37°C Caenorhabditis elegans
0.8
-
 choline N308Q mutant enzyme, pH 10, 37°C Caenorhabditis elegans
1
-
 ATP C252S mutant enzyme, pH 10, 37°C Caenorhabditis elegans
1
-
 choline C252S mutant enzyme, pH 10, 37°C Caenorhabditis elegans
1
-
 choline N308D mutant enzyme, pH 10, 37°C Caenorhabditis elegans
1
-
 ATP N308Q mutant enzyme, pH 10, 37°C Caenorhabditis elegans
1
-
 choline N354A mutant enzyme, pH 10, 37°C Caenorhabditis elegans
1
-
 choline wild type enzyme including His-tag, pH 10, 37°C Caenorhabditis elegans
1.1
-
 choline E125A mutant enzyme, pH 10, 37°C Caenorhabditis elegans
1.2
-
 ATP N354A mutant enzyme, pH 10, 37°C Caenorhabditis elegans
1.2
-
 choline R149K mutant enzyme, pH 10, 37°C Caenorhabditis elegans
1.2
-
 choline W387A mutant enzyme, pH 10, 37°C Caenorhabditis elegans
1.3
-
 ATP D255D mutant enzyme, pH 10, 37°C Caenorhabditis elegans
1.5
-
 choline N308A mutant enzyme, pH 10, 37°C Caenorhabditis elegans
1.5
-
 ATP N308D mutant enzyme, pH 10, 37°C Caenorhabditis elegans
1.6
-
 choline E125Q mutant enzyme, pH 10, 37°C Caenorhabditis elegans
1.6
-
 choline H253A mutant enzyme, pH 10, 37°C Caenorhabditis elegans
1.6
-
 choline wild type enzyme, pH 10, 37°C Caenorhabditis elegans
1.7
-
 ATP E134A mutant enzyme, pH 10, 37°C Caenorhabditis elegans
1.7
-
 ATP N87A mutant enzyme, pH 10, 37°C Caenorhabditis elegans
1.7
-
 choline R111A mutant enzyme, pH 10, 37°C Caenorhabditis elegans
1.7
-
 ATP wild type enzyme including His-tag, pH 10, 37°C Caenorhabditis elegans
1.8
-
 choline E134A mutant enzyme, pH 10, 37°C Caenorhabditis elegans
1.8
-
 choline N87A mutant enzyme, pH 10, 37°C Caenorhabditis elegans
1.9
-
 ATP H253A mutant enzyme, pH 10, 37°C Caenorhabditis elegans
1.9
-
 ATP S85T mutant enzyme, pH 10, 37°C Caenorhabditis elegans
2
-
 ATP N308A mutant enzyme, pH 10, 37°C Caenorhabditis elegans
2.2
-
 ATP E125D mutant enzyme, pH 10, 37°C Caenorhabditis elegans
2.2
-
 choline E151A mutant enzyme, pH 10, 37°C Caenorhabditis elegans
2.2
-
 ATP R149A mutant enzyme, pH 10, 37°C Caenorhabditis elegans
2.2
-
 ATP W387A mutant enzyme, pH 10, 37°C Caenorhabditis elegans
2.3
-
 ATP R149K mutant enzyme, pH 10, 37°C Caenorhabditis elegans
2.4
-
 ATP wild type enzyme, pH 10, 37°C Caenorhabditis elegans
2.5
-
 ATP S86A mutant enzyme, pH 10, 37°C Caenorhabditis elegans
3
-
 choline E320A mutant enzyme, pH 10, 37°C Caenorhabditis elegans
3.1
-
 ATP N260A mutant enzyme, pH 10, 37°C Caenorhabditis elegans
3.2
-
 ATP E125A mutant enzyme, pH 10, 37°C Caenorhabditis elegans
3.4
-
 choline E303Q mutant enzyme, pH 10, 37°C Caenorhabditis elegans
3.6
-
 choline E303D mutant enzyme, pH 10, 37°C Caenorhabditis elegans
4.2
-
 ATP E125Q mutant enzyme, pH 10, 37°C Caenorhabditis elegans
4.5
-
 ATP E151A mutant enzyme, pH 10, 37°C Caenorhabditis elegans
5
-
 choline S86A mutant enzyme, pH 10, 37°C Caenorhabditis elegans
5.4
-
 choline D255D mutant enzyme, pH 10, 37°C Caenorhabditis elegans
5.4
-
 ATP E303A mutant enzyme, pH 10, 37°C Caenorhabditis elegans
6.9
-
 ATP E303D mutant enzyme, pH 10, 37°C Caenorhabditis elegans
7
-
 ATP R111A mutant enzyme, pH 10, 37°C Caenorhabditis elegans
8.8
-
 ATP E303Q mutant enzyme, pH 10, 37°C Caenorhabditis elegans
10
-
 choline E303A mutant enzyme, pH 10, 37°C Caenorhabditis elegans
45
-
 ATP E320A mutant enzyme, pH 10, 37°C Caenorhabditis elegans

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
 Caenorhabditis elegans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
51000
-
 2 * 51000, recombinant enzyme including His-tag, SDS-PAGE, native mass by gel filtration Caenorhabditis elegans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + choline Caenorhabditis elegans initial step of the CDP-choline pathway, that yields phosphatidylcholine as final product, involved in mitogenic signaling transduction ADP + O-phosphocholine
-
 ?

Organism

Organism UniProt Comment Textmining
Caenorhabditis elegans
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzymes using His-tag Caenorhabditis elegans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + choline
-
 Caenorhabditis elegans ADP + O-phosphocholine
-
 ?
ATP + choline initial step of the CDP-choline pathway, that yields phosphatidylcholine as final product, involved in mitogenic signaling transduction Caenorhabditis elegans ADP + O-phosphocholine
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 51000, recombinant enzyme including His-tag, SDS-PAGE, native mass by gel filtration Caenorhabditis elegans

Synonyms

Synonyms Comment Organism
More at least four genes encoding active choline kinase Caenorhabditis elegans

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.24
-
 choline N260A mutant enzyme, pH 10, 37°C Caenorhabditis elegans
0.25
-
 choline D255D mutant enzyme, pH 10, 37°C Caenorhabditis elegans
2.4
-
 choline W387A mutant enzyme, pH 10, 37°C Caenorhabditis elegans
2.6
-
 choline N354A mutant enzyme, pH 10, 37°C Caenorhabditis elegans
7
-
 choline E303A mutant enzyme, pH 10, 37°C Caenorhabditis elegans
9.8
-
 choline S86A mutant enzyme, pH 10, 37°C Caenorhabditis elegans
20.8
-
 choline N308A mutant enzyme, pH 10, 37°C Caenorhabditis elegans
21
-
 choline E125A mutant enzyme, pH 10, 37°C Caenorhabditis elegans
27.1
-
 choline R111A mutant enzyme, pH 10, 37°C Caenorhabditis elegans
29.7
-
 choline N87A mutant enzyme, pH 10, 37°C Caenorhabditis elegans
31.4
-
 choline E125Q mutant enzyme, pH 10, 37°C Caenorhabditis elegans
35.7
-
 choline S85T mutant enzyme, pH 10, 37°C Caenorhabditis elegans
37.5
-
 choline N308Q mutant enzyme, pH 10, 37°C Caenorhabditis elegans
38.7
-
 choline E303D mutant enzyme, pH 10, 37°C Caenorhabditis elegans
38.9
-
 choline R149A mutant enzyme, pH 10, 37°C Caenorhabditis elegans
42.9
-
 choline H253A mutant enzyme, pH 10, 37°C Caenorhabditis elegans
58.7
-
 choline E303Q mutant enzyme, pH 10, 37°C Caenorhabditis elegans
72.3
-
 choline wild type enzyme including His-tag, pH 10, 37°C Caenorhabditis elegans
74
-
 choline wild type enzyme, pH 10, 37°C Caenorhabditis elegans
79.9
-
 choline E320A mutant enzyme, pH 10, 37°C Caenorhabditis elegans
84.3
-
 choline C252S mutant enzyme, pH 10, 37°C Caenorhabditis elegans
84.6
-
 choline N308D mutant enzyme, pH 10, 37°C Caenorhabditis elegans
87.9
-
 choline R149K mutant enzyme, pH 10, 37°C Caenorhabditis elegans
89.2
-
 choline E151A mutant enzyme, pH 10, 37°C Caenorhabditis elegans
97.3
-
 choline E134A mutant enzyme, pH 10, 37°C Caenorhabditis elegans
130
-
 choline E125D mutant enzyme, pH 10, 37°C Caenorhabditis elegans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
10
-
-
 Caenorhabditis elegans