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Literature summary for 2.7.1.32 extracted from
- Expression, purification, and characterization of choline kinase, product of the CKI gene from Saccharomyces cerevisiae (1998), J. Biol. Chem., 273, 6844-6852.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ADP | 50% inhibition at 0.32 mM, in a positive cooperative manner | Saccharomyces cerevisiae |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.09 | - |
ATP | pH 9.5, 30°C | Saccharomyces cerevisiae | |
| 0.27 | - |
choline | pH 9.5, 30°C | Saccharomyces cerevisiae | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Saccharomyces cerevisiae | 5829 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required | Saccharomyces cerevisiae | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 66300 | - |
2 * 73000, SDS-PAGE, 2 * 66300, deduced from gene sequence, enzyme exists also as tetramer and octamer whose amounts increase in the presence of ATP | Saccharomyces cerevisiae |
| 66300 | - |
2 * 73000, SDS-PAGE, 2 * 66300, deduced from gene sequence, enzyme exists also as dimer and octamer, amount of tetramer and octamer increases in the presence of ATP | Saccharomyces cerevisiae |
| 73000 | - |
2 * 73000, SDS-PAGE, 2 * 66300, deduced from gene sequence, enzyme exists also as tetramer and octamer whose amounts increase in the presence of ATP | Saccharomyces cerevisiae |
| 73000 | - |
2 * 73000, SDS-PAGE, 2 * 66300, deduced from gene sequence, enzyme exists also as dimer and octamer, amount of tetramer and octamer increases in the presence of ATP | Saccharomyces cerevisiae |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharomyces cerevisiae | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme, from Sf-9 insect cells | Saccharomyces cerevisiae |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| ATP + choline = ADP + phosphocholine | sequential bi bi reaction | Saccharomyces cerevisiae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + choline | - |
Saccharomyces cerevisiae | ADP + O-phosphocholine | - |
r | |
| ATP + ethanolamine | poor substrate compared to choline | Saccharomyces cerevisiae | ADP + ethanolamine phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 73000, SDS-PAGE, 2 * 66300, deduced from gene sequence, enzyme exists also as tetramer and octamer whose amounts increase in the presence of ATP | Saccharomyces cerevisiae |
| tetramer | 2 * 73000, SDS-PAGE, 2 * 66300, deduced from gene sequence, enzyme exists also as dimer and octamer, amount of tetramer and octamer increases in the presence of ATP | Saccharomyces cerevisiae |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
- |
Saccharomyces cerevisiae |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2.55 | - |
choline | per subunit | Saccharomyces cerevisiae | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9.5 | - |
- |
Saccharomyces cerevisiae |
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