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Literature summary for 2.7.1.26 extracted from
- Role of key residues at the flavin mononucleotide (FMN):adenylyltransferase catalytic site of the bifunctional riboflavin kinase/flavin adenine dinucleotide (FAD) synthetase from Corynebacterium ammoniagenes (2012), Int. J. Mol. Sci., 13, 14492-14517.
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| H28A | the mutant shows reduced catalytic efficiency compared to the wild type enzyme | Corynebacterium ammoniagenes |
| H28D | the mutant shows reduced catalytic efficiency compared to the wild type enzyme | Corynebacterium ammoniagenes |
| H31A | the mutant shows increased catalytic efficiency compared to the wild type enzyme | Corynebacterium ammoniagenes |
| N125A | the mutant shows reduced catalytic efficiency compared to the wild type enzyme | Corynebacterium ammoniagenes |
| N125D | the mutant shows reduced catalytic efficiency compared to the wild type enzyme | Corynebacterium ammoniagenes |
| R161A | the mutant shows reduced catalytic efficiency compared to the wild type enzyme | Corynebacterium ammoniagenes |
| R161D | the mutant shows wild type catalytic efficiency | Corynebacterium ammoniagenes |
| S164A | the mutant shows increased catalytic efficiency compared to the wild type enzyme | Corynebacterium ammoniagenes |
| S164D | the mutant shows increased catalytic efficiency compared to the wild type enzyme | Corynebacterium ammoniagenes |
| T165A | the mutant shows reduced catalytic efficiency compared to the wild type enzyme | Corynebacterium ammoniagenes |
| T165D | the mutant shows wild type catalytic efficiency | Corynebacterium ammoniagenes |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| riboflavin | substrate inhibition | Corynebacterium ammoniagenes |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0017 | - |
riboflavin | mutant enzyme N125A, in 20 mM PIPES, 0.8 mM MgCl2, pH 7.0, at 37°C | Corynebacterium ammoniagenes | |
| 0.0045 | - |
riboflavin | mutant enzyme H31A, in 20 mM PIPES, 0.8 mM MgCl2, pH 7.0, at 37°C | Corynebacterium ammoniagenes | |
| 0.0056 | - |
riboflavin | mutant enzyme S164D, in 20 mM PIPES, 0.8 mM MgCl2, pH 7.0, at 37°C | Corynebacterium ammoniagenes | |
| 0.0077 | - |
riboflavin | mutant enzyme T165D, in 20 mM PIPES, 0.8 mM MgCl2, pH 7.0, at 37°C | Corynebacterium ammoniagenes | |
| 0.0088 | - |
riboflavin | mutant enzyme S164A, in 20 mM PIPES, 0.8 mM MgCl2, pH 7.0, at 37°C | Corynebacterium ammoniagenes | |
| 0.01 | - |
ATP | mutant enzyme S164D, in 20 mM PIPES, 0.8 mM MgCl2, pH 7.0, at 37°C | Corynebacterium ammoniagenes | |
| 0.01 | - |
riboflavin | mutant enzyme T165A, in 20 mM PIPES, 0.8 mM MgCl2, pH 7.0, at 37°C | Corynebacterium ammoniagenes | |
| 0.011 | - |
ATP | mutant enzyme R161D, in 20 mM PIPES, 0.8 mM MgCl2, pH 7.0, at 37°C | Corynebacterium ammoniagenes | |
| 0.011 | - |
ATP | mutant enzyme T165D, in 20 mM PIPES, 0.8 mM MgCl2, pH 7.0, at 37°C | Corynebacterium ammoniagenes | |
| 0.012 | - |
ATP | mutant enzyme H28D, in 20 mM PIPES, 0.8 mM MgCl2, pH 7.0, at 37°C | Corynebacterium ammoniagenes | |
| 0.012 | - |
ATP | mutant enzyme R161A, in 20 mM PIPES, 0.8 mM MgCl2, pH 7.0, at 37°C | Corynebacterium ammoniagenes | |
| 0.012 | - |
riboflavin | mutant enzyme R161A, in 20 mM PIPES, 0.8 mM MgCl2, pH 7.0, at 37°C | Corynebacterium ammoniagenes | |
| 0.012 | - |
ATP | mutant enzyme S164A, in 20 mM PIPES, 0.8 mM MgCl2, pH 7.0, at 37°C | Corynebacterium ammoniagenes | |
| 0.012 | - |
ATP | mutant enzyme T165A, in 20 mM PIPES, 0.8 mM MgCl2, pH 7.0, at 37°C | Corynebacterium ammoniagenes | |
| 0.013 | - |
riboflavin | mutant enzyme R161D, in 20 mM PIPES, 0.8 mM MgCl2, pH 7.0, at 37°C | Corynebacterium ammoniagenes | |
| 0.013 | - |
riboflavin | wild type enzyme, in 20 mM PIPES, 0.8 mM MgCl2, pH 7.0, at 37°C | Corynebacterium ammoniagenes | |
| 0.014 | - |
ATP | mutant enzyme H28A, in 20 mM PIPES, 0.8 mM MgCl2, pH 7.0, at 37°C | Corynebacterium ammoniagenes | |
| 0.014 | - |
ATP | mutant enzyme H31A, in 20 mM PIPES, 0.8 mM MgCl2, pH 7.0, at 37°C | Corynebacterium ammoniagenes | |
| 0.014 | - |
ATP | wild type enzyme, in 20 mM PIPES, 0.8 mM MgCl2, pH 7.0, at 37°C | Corynebacterium ammoniagenes | |
| 0.016 | - |
riboflavin | mutant enzyme N125D, in 20 mM PIPES, 0.8 mM MgCl2, pH 7.0, at 37°C | Corynebacterium ammoniagenes | |
| 0.023 | - |
riboflavin | mutant enzyme H28A, in 20 mM PIPES, 0.8 mM MgCl2, pH 7.0, at 37°C | Corynebacterium ammoniagenes | |
| 0.024 | - |
ATP | mutant enzyme N125A, in 20 mM PIPES, 0.8 mM MgCl2, pH 7.0, at 37°C | Corynebacterium ammoniagenes | |
| 0.025 | - |
riboflavin | mutant enzyme H28D, in 20 mM PIPES, 0.8 mM MgCl2, pH 7.0, at 37°C | Corynebacterium ammoniagenes | |
| 0.035 | - |
ATP | mutant enzyme N125D, in 20 mM PIPES, 0.8 mM MgCl2, pH 7.0, at 37°C | Corynebacterium ammoniagenes | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Corynebacterium ammoniagenes | Q59263 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + riboflavin | - |
Corynebacterium ammoniagenes | ADP + FMN | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ATP:riboflavin kinase | - |
Corynebacterium ammoniagenes |
| FAD synthetase | bifunctional enzyme, the C-terminal is associated with ATP:riboflavin kinase activity and the N-terminal associated with ATP:FMN adenylyltransferase activity | Corynebacterium ammoniagenes |
| FADS | - |
Corynebacterium ammoniagenes |
| RFK | - |
Corynebacterium ammoniagenes |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.75 | - |
ATP | mutant enzyme H28A, in 20 mM PIPES, 0.8 mM MgCl2, pH 7.0, at 37°C | Corynebacterium ammoniagenes | |
| 0.75 | - |
ATP | mutant enzyme T165D, in 20 mM PIPES, 0.8 mM MgCl2, pH 7.0, at 37°C | Corynebacterium ammoniagenes | |
| 0.77 | - |
ATP | mutant enzyme N125A, in 20 mM PIPES, 0.8 mM MgCl2, pH 7.0, at 37°C | Corynebacterium ammoniagenes | |
| 0.92 | - |
ATP | mutant enzyme H28D, in 20 mM PIPES, 0.8 mM MgCl2, pH 7.0, at 37°C | Corynebacterium ammoniagenes | |
| 0.92 | - |
ATP | mutant enzyme S164D, in 20 mM PIPES, 0.8 mM MgCl2, pH 7.0, at 37°C | Corynebacterium ammoniagenes | |
| 0.97 | - |
ATP | mutant enzyme H31A, in 20 mM PIPES, 0.8 mM MgCl2, pH 7.0, at 37°C | Corynebacterium ammoniagenes | |
| 0.98 | - |
ATP | mutant enzyme S164A, in 20 mM PIPES, 0.8 mM MgCl2, pH 7.0, at 37°C | Corynebacterium ammoniagenes | |
| 1.08 | - |
ATP | mutant enzyme R161A, in 20 mM PIPES, 0.8 mM MgCl2, pH 7.0, at 37°C | Corynebacterium ammoniagenes | |
| 1.1 | - |
ATP | mutant enzyme T165A, in 20 mM PIPES, 0.8 mM MgCl2, pH 7.0, at 37°C | Corynebacterium ammoniagenes | |
| 1.13 | - |
ATP | mutant enzyme R161D, in 20 mM PIPES, 0.8 mM MgCl2, pH 7.0, at 37°C | Corynebacterium ammoniagenes | |
| 1.13 | - |
ATP | wild type enzyme, in 20 mM PIPES, 0.8 mM MgCl2, pH 7.0, at 37°C | Corynebacterium ammoniagenes | |
| 1.33 | - |
ATP | mutant enzyme N125D, in 20 mM PIPES, 0.8 mM MgCl2, pH 7.0, at 37°C | Corynebacterium ammoniagenes | |
| 1.85 | - |
riboflavin | mutant enzyme N125A, in 20 mM PIPES, 0.8 mM MgCl2, pH 7.0, at 37°C | Corynebacterium ammoniagenes | |
| 2.82 | - |
riboflavin | mutant enzyme H31A, in 20 mM PIPES, 0.8 mM MgCl2, pH 7.0, at 37°C | Corynebacterium ammoniagenes | |
| 2.85 | - |
riboflavin | mutant enzyme T165A, in 20 mM PIPES, 0.8 mM MgCl2, pH 7.0, at 37°C | Corynebacterium ammoniagenes | |
| 3 | - |
riboflavin | mutant enzyme T165D, in 20 mM PIPES, 0.8 mM MgCl2, pH 7.0, at 37°C | Corynebacterium ammoniagenes | |
| 3.08 | - |
riboflavin | mutant enzyme S164D, in 20 mM PIPES, 0.8 mM MgCl2, pH 7.0, at 37°C | Corynebacterium ammoniagenes | |
| 4.32 | - |
riboflavin | mutant enzyme S164A, in 20 mM PIPES, 0.8 mM MgCl2, pH 7.0, at 37°C | Corynebacterium ammoniagenes | |
| 4.78 | - |
riboflavin | mutant enzyme H28D, in 20 mM PIPES, 0.8 mM MgCl2, pH 7.0, at 37°C | Corynebacterium ammoniagenes | |
| 5 | - |
riboflavin | mutant enzyme R161A, in 20 mM PIPES, 0.8 mM MgCl2, pH 7.0, at 37°C | Corynebacterium ammoniagenes | |
| 5 | - |
riboflavin | mutant enzyme R161D, in 20 mM PIPES, 0.8 mM MgCl2, pH 7.0, at 37°C | Corynebacterium ammoniagenes | |
| 5.02 | - |
riboflavin | wild type enzyme, in 20 mM PIPES, 0.8 mM MgCl2, pH 7.0, at 37°C | Corynebacterium ammoniagenes | |
| 6.92 | - |
riboflavin | mutant enzyme N125D, in 20 mM PIPES, 0.8 mM MgCl2, pH 7.0, at 37°C | Corynebacterium ammoniagenes | |
| 7.12 | - |
riboflavin | mutant enzyme H28A, in 20 mM PIPES, 0.8 mM MgCl2, pH 7.0, at 37°C | Corynebacterium ammoniagenes | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0018 | - |
riboflavin | mutant enzyme H28A, in 20 mM PIPES, 0.8 mM MgCl2, pH 7.0, at 37°C | Corynebacterium ammoniagenes | |
| 0.0019 | - |
riboflavin | mutant enzyme H28D, in 20 mM PIPES, 0.8 mM MgCl2, pH 7.0, at 37°C | Corynebacterium ammoniagenes | |
| 0.0032 | - |
riboflavin | mutant enzyme T165A, in 20 mM PIPES, 0.8 mM MgCl2, pH 7.0, at 37°C | Corynebacterium ammoniagenes | |
| 0.0033 | - |
riboflavin | mutant enzyme N125D, in 20 mM PIPES, 0.8 mM MgCl2, pH 7.0, at 37°C | Corynebacterium ammoniagenes | |
| 0.004 | - |
riboflavin | wild type enzyme, in 20 mM PIPES, 0.8 mM MgCl2, pH 7.0, at 37°C | Corynebacterium ammoniagenes | |
| 0.0041 | - |
riboflavin | mutant enzyme T165D, in 20 mM PIPES, 0.8 mM MgCl2, pH 7.0, at 37°C | Corynebacterium ammoniagenes | |
| 0.0044 | - |
riboflavin | mutant enzyme S164A, in 20 mM PIPES, 0.8 mM MgCl2, pH 7.0, at 37°C | Corynebacterium ammoniagenes | |
| 0.0046 | - |
riboflavin | mutant enzyme N125A, in 20 mM PIPES, 0.8 mM MgCl2, pH 7.0, at 37°C | Corynebacterium ammoniagenes | |
| 0.0051 | - |
riboflavin | mutant enzyme R161A, in 20 mM PIPES, 0.8 mM MgCl2, pH 7.0, at 37°C | Corynebacterium ammoniagenes | |
| 0.0051 | - |
riboflavin | mutant enzyme R161D, in 20 mM PIPES, 0.8 mM MgCl2, pH 7.0, at 37°C | Corynebacterium ammoniagenes | |
| 0.0058 | - |
riboflavin | mutant enzyme S164D, in 20 mM PIPES, 0.8 mM MgCl2, pH 7.0, at 37°C | Corynebacterium ammoniagenes | |
| 0.0063 | - |
riboflavin | mutant enzyme H31A, in 20 mM PIPES, 0.8 mM MgCl2, pH 7.0, at 37°C | Corynebacterium ammoniagenes | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 31.7 | - |
ATP | mutant enzyme N125A, in 20 mM PIPES, 0.8 mM MgCl2, pH 7.0, at 37°C | Corynebacterium ammoniagenes | |
| 38.3 | - |
ATP | mutant enzyme N125D, in 20 mM PIPES, 0.8 mM MgCl2, pH 7.0, at 37°C | Corynebacterium ammoniagenes | |
| 51.7 | - |
ATP | mutant enzyme H28A, in 20 mM PIPES, 0.8 mM MgCl2, pH 7.0, at 37°C | Corynebacterium ammoniagenes | |
| 68.3 | - |
ATP | mutant enzyme T165D, in 20 mM PIPES, 0.8 mM MgCl2, pH 7.0, at 37°C | Corynebacterium ammoniagenes | |
| 71.7 | - |
ATP | mutant enzyme H31A, in 20 mM PIPES, 0.8 mM MgCl2, pH 7.0, at 37°C | Corynebacterium ammoniagenes | |
| 78.3 | - |
ATP | mutant enzyme H28D, in 20 mM PIPES, 0.8 mM MgCl2, pH 7.0, at 37°C | Corynebacterium ammoniagenes | |
| 81.7 | - |
ATP | wild type enzyme, in 20 mM PIPES, 0.8 mM MgCl2, pH 7.0, at 37°C | Corynebacterium ammoniagenes | |
| 83.3 | - |
ATP | mutant enzyme S164A, in 20 mM PIPES, 0.8 mM MgCl2, pH 7.0, at 37°C | Corynebacterium ammoniagenes | |
| 88.3 | - |
ATP | mutant enzyme S164D, in 20 mM PIPES, 0.8 mM MgCl2, pH 7.0, at 37°C | Corynebacterium ammoniagenes | |
| 90 | - |
ATP | mutant enzyme T165A, in 20 mM PIPES, 0.8 mM MgCl2, pH 7.0, at 37°C | Corynebacterium ammoniagenes | |
| 93.3 | - |
ATP | mutant enzyme R161A, in 20 mM PIPES, 0.8 mM MgCl2, pH 7.0, at 37°C | Corynebacterium ammoniagenes | |
| 101.7 | - |
ATP | mutant enzyme R161D, in 20 mM PIPES, 0.8 mM MgCl2, pH 7.0, at 37°C | Corynebacterium ammoniagenes | |
| 200 | - |
riboflavin | mutant enzyme H28D, in 20 mM PIPES, 0.8 mM MgCl2, pH 7.0, at 37°C | Corynebacterium ammoniagenes | |
| 283 | - |
riboflavin | mutant enzyme T165A, in 20 mM PIPES, 0.8 mM MgCl2, pH 7.0, at 37°C | Corynebacterium ammoniagenes | |
| 300 | - |
riboflavin | mutant enzyme H28A, in 20 mM PIPES, 0.8 mM MgCl2, pH 7.0, at 37°C | Corynebacterium ammoniagenes | |
| 383 | - |
riboflavin | mutant enzyme R161D, in 20 mM PIPES, 0.8 mM MgCl2, pH 7.0, at 37°C | Corynebacterium ammoniagenes | |
| 383 | - |
riboflavin | mutant enzyme T165D, in 20 mM PIPES, 0.8 mM MgCl2, pH 7.0, at 37°C | Corynebacterium ammoniagenes | |
| 383 | - |
riboflavin | wild type enzyme, in 20 mM PIPES, 0.8 mM MgCl2, pH 7.0, at 37°C | Corynebacterium ammoniagenes | |
| 417 | - |
riboflavin | mutant enzyme R161A, in 20 mM PIPES, 0.8 mM MgCl2, pH 7.0, at 37°C | Corynebacterium ammoniagenes | |
| 433 | - |
riboflavin | mutant enzyme N125D, in 20 mM PIPES, 0.8 mM MgCl2, pH 7.0, at 37°C | Corynebacterium ammoniagenes | |
| 483 | - |
riboflavin | mutant enzyme S164A, in 20 mM PIPES, 0.8 mM MgCl2, pH 7.0, at 37°C | Corynebacterium ammoniagenes | |
| 550 | - |
riboflavin | mutant enzyme S164D, in 20 mM PIPES, 0.8 mM MgCl2, pH 7.0, at 37°C | Corynebacterium ammoniagenes | |
| 617 | - |
riboflavin | mutant enzyme H31A, in 20 mM PIPES, 0.8 mM MgCl2, pH 7.0, at 37°C | Corynebacterium ammoniagenes | |
| 1083 | - |
riboflavin | mutant enzyme N125A, in 20 mM PIPES, 0.8 mM MgCl2, pH 7.0, at 37°C | Corynebacterium ammoniagenes | |
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