General Stability | Organism |
---|---|
loss of activity during repeated freezing and thawing | Megasphaera elsdenii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0047 | - |
riboflavin | pH 7.2, 37°C | Megasphaera elsdenii | |
0.01 | - |
riboflavin | pH 7.2, 37°C | Megasphaera elsdenii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Megasphaera elsdenii | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0083 | - |
continuous fluorometric assay | Megasphaera elsdenii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ADP + riboflavin | 22% of the activity with ATP | Megasphaera elsdenii | AMP + FMN | - |
? | |
ATP + 5-deazariboflavin | - |
Megasphaera elsdenii | ADP + 5-deazariboflavin 5'-phosphate | - |
? | |
ATP + riboflavin | - |
Megasphaera elsdenii | ADP + FMN | - |
? | |
GTP + riboflavin | no activity | Megasphaera elsdenii | GDP + riboflavin 5'-phosphate | - |
? |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 45 | the relative activities at pH 7.2 and 20°C, 25°C, 37°C and 45°C are 0.22, 0.46, 1, and 1.35, respectively | Megasphaera elsdenii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Megasphaera elsdenii |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 8.5 | enzyme is active between pH 5 and 8.5 | Megasphaera elsdenii |