Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.7.1.24 extracted from

  • O'Toole, N.; Barbosa, J.A.; Li, Y.; Hung, L.W.; Matte, A.; Cygler, M.
    Crystal structure of a trimeric form of dephosphocoenzyme A kinase from Escherichia coli (2003), Protein Sci., 12, 327-336.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.1.24

Cloned(Commentary)

Cloned (Comment) Organism
gene coaE, expression as His-tagged protein in strain DL41 Escherichia coli

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapour diffusion method, purified enzyme in 20 mM Tris-HCl, pH 7.5, 0.2 M NaCl, 5% glycerol v/v, 10 mM DTT, from droplets of equal volume of protein solution and reservoir solution, reservoir solution: 20% w/v PEG 8K, 50 mM cacodylate, pH 6.5, 0.2 M (NH4)2SO4, 5% glycerol v/v, 21°C, within 4 days to 2 weeks, X-ray and light scattering structure determination and analysis Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
22000
-
 monomeric enzyme form, gel filtration Escherichia coli
66000
-
 trimeric enzyme form, gel filtration Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + dephospho-CoA Escherichia coli final step of coenzyme A biosynthesis ADP + CoA
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from strain DL41 Escherichia coli

Reaction

Reaction Comment Organism Reaction ID
ATP + 3'-dephospho-CoA = ADP + CoA active site, mechanism Escherichia coli
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + dephospho-CoA
-
 Escherichia coli ADP + CoA
-
 ?
ATP + dephospho-CoA final step of coenzyme A biosynthesis Escherichia coli ADP + CoA
-
 ?

Subunits

Subunits Comment Organism
More enzyme is a monomer in solution, but crystallizes as a tightly packed trimer, in presence of stabilizing sulfate ions the monomeric and trimeric forms build an equilibrium in solution Escherichia coli

Synonyms

Synonyms Comment Organism
DPCK
-
 Escherichia coli