Cloned (Comment) | Organism |
---|---|
gene ppat/dpck encoding bifunctional enzyme with phosphopantetheine adenylyltransferase and dephospho-CoA kinase activity, located on chromosome 17q12-21, DNA sequence determination and analysis, expression in Escherichia coli BL21(DE3) | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0041 | - |
dephospho-CoA | native enzyme, pH 8.0, 25°C | Sus scrofa | |
0.0052 | - |
dephospho-CoA | recombinant enzyme, pH 8.0, 25°C | Homo sapiens | |
0.19 | - |
ATP | recombinant enzyme, pH 8.0, 25°C | Homo sapiens | |
0.33 | - |
ATP | recombinant enzyme, pH 8.0, 25°C | Sus scrofa |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Sus scrofa | 5829 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
60000 | - |
1 * 60000, SDS-PAGE | Sus scrofa |
62000 | - |
recombinant enzyme, gel filtration | Homo sapiens |
62000 | - |
wild-type enzyme, gel filtration | Sus scrofa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q13057 | gene ppat/dpck, bifunctional enzyme with phosphopantetheine adenylyltransferase and dephospho-CoA kinase activities, termed CoA synthase | - |
Sus scrofa | Q8MIR4 | bifunctional enzyme with phosphopantetheine adenylyltransferase and dephospho-CoA kinase activities | - |
Purification (Comment) | Organism |
---|---|
- |
Sus scrofa |
recombinant from Escherichia coli | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
Hep-G2 cell | - |
Homo sapiens | - |
liver | - |
Sus scrofa | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + dephospho-CoA | - |
Homo sapiens | ADP + CoA | - |
? | |
ATP + dephospho-CoA | - |
Sus scrofa | ADP + CoA | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 60000, SDS-PAGE | Sus scrofa |
Synonyms | Comment | Organism |
---|---|---|
DPCK | - |
Homo sapiens |
More | bifunctional enzyme is termed CoA synthase | Homo sapiens |
More | bifunctional enzyme with phosphopantetheine adenylyltransferase activity, EC 2.7.7.3, and dephospho-CoA kinase activity, EC 2.7.1.24 | Homo sapiens |
More | bifunctional enzyme with phosphopantetheine adenylyltransferase activity, EC 2.7.7.3, and dephospho-CoA kinase activity, EC 2.7.1.24 | Sus scrofa |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Homo sapiens |
25 | - |
assay at | Sus scrofa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Homo sapiens |
8 | - |
assay at | Sus scrofa |