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Literature summary for 2.7.1.21 extracted from
- Identification and characterization of mitochondrial factors modulating thymidine kinase 2 activity (2010), Nucleosides Nucleotides Nucleic Acids, 29, 382-385.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| 3'-azido-2',3'-dideoxy-3,4-dihydrothymidine | stimulates deoxycytidine phosphorylation catalyzed by thymidine kinase 2 | Rattus norvegicus |  |
| 3'-azido-3'-deoxythymidine | stimulates deoxycytidine phosphorylation catalyzed by thymidine kinase 2 | Homo sapiens | |
| 3'-deoxy-3'-fluorothymidine | stimulates deoxycytidine phosphorylation catalyzed by thymidine kinase 2 | Homo sapiens | |
| 3'-fluorothymidine | stimulates deoxycytidine phosphorylation catalyzed by thymidine kinase 2 | Rattus norvegicus | |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 3'-azido-2',3'-dideoxy-3,4-dihydrothymidine | inhibits thymidine phosphorylation catalyzed by thymidine kinase 2 | Homo sapiens | |
| 3'-azido-2',3'-dideoxy-3,4-dihydrothymidine | inhibits thymidine phosphorylation catalyzed by thymidine kinase 2 | Rattus norvegicus | |
| 3'-deoxy-3'-fluorothymidine | inhibits thymidine phosphorylation catalyzed by thymidine kinase 2 | Homo sapiens | |
| 3'-fluorothymidine | inhibits thymidine phosphorylation catalyzed by thymidine kinase 2 | Rattus norvegicus | |
| additional information | at physiological relevant concentration deoxycytidine phosphorylation is not inhibited by 3-azido-3'-deoxythmidine or 3'-fluorothymidine | Homo sapiens | |
| additional information | at physiological relevant concentration deoxycytidine phosphorylation is not inhibited by 3'-azido-3'-deoxythmidine or 3'-fluorothymidine | Rattus norvegicus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0188 | - |
thymidine | pH and temperature not specified in the publication | Rattus norvegicus | |
| 0.0414 | - |
deoxycytidine | pH and temperature not specified in the publication | Rattus norvegicus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| mitochondrion | - |
Homo sapiens | 5739 | - |
| mitochondrion | - |
Rattus norvegicus | 5739 | - |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
| Rattus norvegicus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Homo sapiens |
| DEAE-Sephadex gel filtration | Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Homo sapiens | - |
| liver | - |
Rattus norvegicus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + deoxycytidine | - |
Homo sapiens | ADP + dCMP | - |
? | |
| ATP + deoxycytidine | - |
Rattus norvegicus | ADP + dCMP | - |
? | |
| ATP + thymidine | - |
Homo sapiens | ADP + dTMP | - |
? | |
| ATP + thymidine | - |
Rattus norvegicus | ADP + dTMP | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| thymidine kinase 2 | - |
Homo sapiens |
| thymidine kinase 2 | - |
Rattus norvegicus |
| TK2 | - |
Homo sapiens |
| TK2 | - |
Rattus norvegicus |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0014 | - |
3'-deoxy-3'-fluorothymidine | pH and temperature not specified in the publication | Homo sapiens | |
| 0.0014 | - |
3'-fluorothymidine | pH and temperature not specified in the publication | Rattus norvegicus | |
| 0.0039 | - |
3'-azido-2',3'-dideoxy-3,4-dihydrothymidine | pH and temperature not specified in the publication | Homo sapiens | |
| 0.0039 | - |
3'-azido-2',3'-dideoxy-3,4-dihydrothymidine | pH and temperature not specified in the publication | Rattus norvegicus | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | thymidine kinase 2 plays an important role in providing precursors for the replication and maintenance of mitochondrial DNA, especially in nonproliferating cells | Homo sapiens |
| physiological function | thymidine kinase 2 plays an important role in providing precursors for the replication and maintenance of mitochondrial DNA, especially in nonproliferating cells | Rattus norvegicus |
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