Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.7.1.21 extracted from

  • Sun, X.E.; Sharling, L.; Muthalagi, M.; Mudeppa, D.G.; Pankiewicz, K.W.; Felczak, K.; Rathod, P.K.; Mead, J.; Striepen, B.; Hedstrom, L.
    Prodrug activation by Cryptosporidium thymidine kinase (2010), J. Biol. Chem., 285, 15916-15922.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
dCDP dCDP activates the enzyme 2.5old Cryptosporidium parvum
dCTP dCTP is not a phosphate donor, but instead activates the enzyme 7fold Cryptosporidium parvum
additional information the enzyme is not activated by dATP or dGTP Cryptosporidium parvum

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Cryptosporidium parvum

Inhibitors

Inhibitors Comment Organism Structure
dTTP
-
Cryptosporidium parvum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.022
-
trifluoromethylthymidine at pH 7.8 and 25°C Cryptosporidium parvum
0.032
-
3'-azido-2',3'-dideoxythymidine at pH 7.8 and 25°C Cryptosporidium parvum
0.04
-
5-fluoro-2'-deoxyuridine at pH 7.8 and 25°C Cryptosporidium parvum
0.048
-
thymidine at pH 7.8 and 25°C Cryptosporidium parvum
0.05
-
2',3'-didehydro-2',3'-dideoxythymidine at pH 7.8 and 25°C Cryptosporidium parvum
0.118
-
deoxyuridine at pH 7.8 and 25°C Cryptosporidium parvum
0.14
-
ATP at pH 7.8 and 25°C Cryptosporidium parvum
0.29
-
beta-L-thymidine at pH 7.8 and 25°C Cryptosporidium parvum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
23300
-
3 * 23300, calculated from amino acid sequence Cryptosporidium parvum
70000
-
gel filtration Cryptosporidium parvum

Organism

Organism UniProt Comment Textmining
Cryptosporidium parvum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
3'-aminothymidine-Sepharose resin column chromatography and HisTrap column chromatography Cryptosporidium parvum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 2',3'-didehydro-2',3'-dideoxythymidine 12% activity compared to thymidine Cryptosporidium parvum ADP + 2',3'-didehydro-2',3'-dideoxythymidine 5'-phosphate
-
?
ATP + 3'-azido-2',3'-dideoxythymidine 60% activity compared to thymidine Cryptosporidium parvum ADP + 3'-azido-2',3'-dideoxythymidine 5'-phosphate
-
?
ATP + 5-bromodeoxyuridine 84% activity compared to thymidine Cryptosporidium parvum ADP + 5-bromodeoxyuridine 5'-phosphate
-
?
ATP + 5-chlorodeoxyuridine 110% activity compared to thymidine Cryptosporidium parvum ADP + 5-chlorodeoxyuridine 5'-phosphate
-
?
ATP + 5-fluoro-2'-deoxyuridine good substrate, 120% activity compared to thymidine Cryptosporidium parvum ADP + 5-fluoro-2'-deoxyuridine 5'-phosphate
-
?
ATP + 5-iododeoxyuridine 89% activity compared to thymidine Cryptosporidium parvum ADP + 5-iododeoxyuridine 5'-phosphate
-
?
ATP + beta-L-thymidine less than 5% activity compared to thymidine Cryptosporidium parvum ADP + beta-L-thymidine 5'-phosphate
-
?
ATP + deoxycytidine 7% activity compared to thymidine Cryptosporidium parvum ADP + dCMP
-
?
ATP + deoxyuridine 69% activity compared to thymidine Cryptosporidium parvum ADP + dUMP
-
?
ATP + guanosine 1% activity compared to thymidine Cryptosporidium parvum ADP + GMP
-
?
ATP + thymidine 100% activity Cryptosporidium parvum ADP + dTMP
-
?
ATP + trifluoromethylthymidine good substrate, 130% activity compared to thymidine Cryptosporidium parvum ADP + trifluoromethylthymidine 5’-phosphate
-
?
dGTP + thymidine 99% activity compared to ATP Cryptosporidium parvum dGDP + dTMP
-
?
GTP + thymidine 42% activity compared to ATP Cryptosporidium parvum GDP + dTMP
-
?
additional information CTP, diphosphate, uridine, adenosine, cytosine, deoxyguanosine, deoxyguanosine, dTMP, thymine 1-beta-D-arabinofuranoside, 1-beta-D-arabinofuranosylcytosine, acyclovir, and 5-iodo-2'-deoxycytidine are no substrates Cryptosporidium parvum ?
-
?
UTP + thymidine 17% activity compared to ATP Cryptosporidium parvum UDP + dTMP
-
?

Subunits

Subunits Comment Organism
trimer 3 * 23300, calculated from amino acid sequence Cryptosporidium parvum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.007
-
beta-L-thymidine at pH 7.8 and 25°C Cryptosporidium parvum
0.03
-
2',3'-didehydro-2',3'-dideoxythymidine at pH 7.8 and 25°C Cryptosporidium parvum
0.17
-
3'-azido-2',3'-dideoxythymidine at pH 7.8 and 25°C Cryptosporidium parvum
0.24
-
5-fluoro-2'-deoxyuridine at pH 7.8 and 25°C Cryptosporidium parvum
0.28
-
ATP at pH 7.8 and 25°C Cryptosporidium parvum
0.28
-
thymidine at pH 7.8 and 25°C Cryptosporidium parvum
0.36
-
trifluoromethylthymidine at pH 7.8 and 25°C Cryptosporidium parvum
0.53
-
deoxyuridine at pH 7.8 and 25°C Cryptosporidium parvum

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.0024
-
beta-L-thymidine at pH 7.8 and 25°C Cryptosporidium parvum
0.064
-
2',3'-didehydro-2',3'-dideoxythymidine at pH 7.8 and 25°C Cryptosporidium parvum
2.3
-
ATP at pH 7.8 and 25°C Cryptosporidium parvum
4.5
-
deoxyuridine at pH 7.8 and 25°C Cryptosporidium parvum
4.8
-
thymidine at pH 7.8 and 25°C Cryptosporidium parvum
5
-
3'-azido-2',3'-dideoxythymidine at pH 7.8 and 25°C Cryptosporidium parvum
5.8
-
5-fluoro-2'-deoxyuridine at pH 7.8 and 25°C Cryptosporidium parvum
6.4
-
trifluoromethylthymidine at pH 7.8 and 25°C Cryptosporidium parvum