Activating Compound | Comment | Organism | Structure |
---|---|---|---|
dCDP | dCDP activates the enzyme 2.5old | Cryptosporidium parvum | |
dCTP | dCTP is not a phosphate donor, but instead activates the enzyme 7fold | Cryptosporidium parvum | |
additional information | the enzyme is not activated by dATP or dGTP | Cryptosporidium parvum |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Cryptosporidium parvum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
dTTP | - |
Cryptosporidium parvum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.022 | - |
trifluoromethylthymidine | at pH 7.8 and 25°C | Cryptosporidium parvum | |
0.032 | - |
3'-azido-2',3'-dideoxythymidine | at pH 7.8 and 25°C | Cryptosporidium parvum | |
0.04 | - |
5-fluoro-2'-deoxyuridine | at pH 7.8 and 25°C | Cryptosporidium parvum | |
0.048 | - |
thymidine | at pH 7.8 and 25°C | Cryptosporidium parvum | |
0.05 | - |
2',3'-didehydro-2',3'-dideoxythymidine | at pH 7.8 and 25°C | Cryptosporidium parvum | |
0.118 | - |
deoxyuridine | at pH 7.8 and 25°C | Cryptosporidium parvum | |
0.14 | - |
ATP | at pH 7.8 and 25°C | Cryptosporidium parvum | |
0.29 | - |
beta-L-thymidine | at pH 7.8 and 25°C | Cryptosporidium parvum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
23300 | - |
3 * 23300, calculated from amino acid sequence | Cryptosporidium parvum |
70000 | - |
gel filtration | Cryptosporidium parvum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cryptosporidium parvum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
3'-aminothymidine-Sepharose resin column chromatography and HisTrap column chromatography | Cryptosporidium parvum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 2',3'-didehydro-2',3'-dideoxythymidine | 12% activity compared to thymidine | Cryptosporidium parvum | ADP + 2',3'-didehydro-2',3'-dideoxythymidine 5'-phosphate | - |
? | |
ATP + 3'-azido-2',3'-dideoxythymidine | 60% activity compared to thymidine | Cryptosporidium parvum | ADP + 3'-azido-2',3'-dideoxythymidine 5'-phosphate | - |
? | |
ATP + 5-bromodeoxyuridine | 84% activity compared to thymidine | Cryptosporidium parvum | ADP + 5-bromodeoxyuridine 5'-phosphate | - |
? | |
ATP + 5-chlorodeoxyuridine | 110% activity compared to thymidine | Cryptosporidium parvum | ADP + 5-chlorodeoxyuridine 5'-phosphate | - |
? | |
ATP + 5-fluoro-2'-deoxyuridine | good substrate, 120% activity compared to thymidine | Cryptosporidium parvum | ADP + 5-fluoro-2'-deoxyuridine 5'-phosphate | - |
? | |
ATP + 5-iododeoxyuridine | 89% activity compared to thymidine | Cryptosporidium parvum | ADP + 5-iododeoxyuridine 5'-phosphate | - |
? | |
ATP + beta-L-thymidine | less than 5% activity compared to thymidine | Cryptosporidium parvum | ADP + beta-L-thymidine 5'-phosphate | - |
? | |
ATP + deoxycytidine | 7% activity compared to thymidine | Cryptosporidium parvum | ADP + dCMP | - |
? | |
ATP + deoxyuridine | 69% activity compared to thymidine | Cryptosporidium parvum | ADP + dUMP | - |
? | |
ATP + guanosine | 1% activity compared to thymidine | Cryptosporidium parvum | ADP + GMP | - |
? | |
ATP + thymidine | 100% activity | Cryptosporidium parvum | ADP + dTMP | - |
? | |
ATP + trifluoromethylthymidine | good substrate, 130% activity compared to thymidine | Cryptosporidium parvum | ADP + trifluoromethylthymidine 5-phosphate | - |
? | |
dGTP + thymidine | 99% activity compared to ATP | Cryptosporidium parvum | dGDP + dTMP | - |
? | |
GTP + thymidine | 42% activity compared to ATP | Cryptosporidium parvum | GDP + dTMP | - |
? | |
additional information | CTP, diphosphate, uridine, adenosine, cytosine, deoxyguanosine, deoxyguanosine, dTMP, thymine 1-beta-D-arabinofuranoside, 1-beta-D-arabinofuranosylcytosine, acyclovir, and 5-iodo-2'-deoxycytidine are no substrates | Cryptosporidium parvum | ? | - |
? | |
UTP + thymidine | 17% activity compared to ATP | Cryptosporidium parvum | UDP + dTMP | - |
? |
Subunits | Comment | Organism |
---|---|---|
trimer | 3 * 23300, calculated from amino acid sequence | Cryptosporidium parvum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.007 | - |
beta-L-thymidine | at pH 7.8 and 25°C | Cryptosporidium parvum | |
0.03 | - |
2',3'-didehydro-2',3'-dideoxythymidine | at pH 7.8 and 25°C | Cryptosporidium parvum | |
0.17 | - |
3'-azido-2',3'-dideoxythymidine | at pH 7.8 and 25°C | Cryptosporidium parvum | |
0.24 | - |
5-fluoro-2'-deoxyuridine | at pH 7.8 and 25°C | Cryptosporidium parvum | |
0.28 | - |
ATP | at pH 7.8 and 25°C | Cryptosporidium parvum | |
0.28 | - |
thymidine | at pH 7.8 and 25°C | Cryptosporidium parvum | |
0.36 | - |
trifluoromethylthymidine | at pH 7.8 and 25°C | Cryptosporidium parvum | |
0.53 | - |
deoxyuridine | at pH 7.8 and 25°C | Cryptosporidium parvum |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0024 | - |
beta-L-thymidine | at pH 7.8 and 25°C | Cryptosporidium parvum | |
0.064 | - |
2',3'-didehydro-2',3'-dideoxythymidine | at pH 7.8 and 25°C | Cryptosporidium parvum | |
2.3 | - |
ATP | at pH 7.8 and 25°C | Cryptosporidium parvum | |
4.5 | - |
deoxyuridine | at pH 7.8 and 25°C | Cryptosporidium parvum | |
4.8 | - |
thymidine | at pH 7.8 and 25°C | Cryptosporidium parvum | |
5 | - |
3'-azido-2',3'-dideoxythymidine | at pH 7.8 and 25°C | Cryptosporidium parvum | |
5.8 | - |
5-fluoro-2'-deoxyuridine | at pH 7.8 and 25°C | Cryptosporidium parvum | |
6.4 | - |
trifluoromethylthymidine | at pH 7.8 and 25°C | Cryptosporidium parvum |