Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Entamoeba histolytica |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
3'-ethyl-5-methyl-2'-deoxyuridine | - |
Entamoeba histolytica | |
5-iodo-2'-deoxyuridine | potent inhibitor | Entamoeba histolytica | |
5-propyl-2'-deoxyuridine | - |
Entamoeba histolytica | |
5-trifluoromethyl-2-deoxyuridine | potent inhibitor | Entamoeba histolytica | |
6-methyluridine | - |
Entamoeba histolytica | |
alpha-D-5-ethyl-2'-deoxyuridine | - |
Entamoeba histolytica | |
alpha-L-thymidine | - |
Entamoeba histolytica | |
beta-D-5-ethyl-2'-deoxyuridine | potent inhibitor | Entamoeba histolytica | |
beta-L-thymidine | potent inhibitor | Entamoeba histolytica | |
additional information | not inhibited by D-5-(bromovinyl)-2'-deoxyuridine, L-5-(bromovinyl)-2'-deoxyuridine, and 2-phenylamino-9-(4-hydroxybutyl)-6-oxopurine | Entamoeba histolytica | |
penciclovir | - |
Entamoeba histolytica |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00027 | - |
thymidine | at 37°C for 20 min in 30 mM potassium phosphate, pH 8.0, 1.2 mM MgCl2 | Entamoeba histolytica |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | MgCl2 is required for activity, reaching its optimal effect at 1.2 mM when ATP is present at 1 mM | Entamoeba histolytica | |
Mn2+ | MnCl2 can replace MgCl2 in the assay, with optimal activity at 200 mM when ATP is present at 1 mM | Entamoeba histolytica |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Entamoeba histolytica | Q6S4W2 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography | Entamoeba histolytica |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 5-iodo-2'-deoxyuridine | 5-iodo-2'-deoxyuridine is efficiently phosphorylated by thymidine kinase confirming that these compound enters the active site of the enzyme and acts as alternate substrate | Entamoeba histolytica | ? | - |
? | |
ATP + 5-trifluoromethyl-2-deoxyuridine | 5-trifluoromethyl-2-deoxyuridine is efficiently phosphorylated by thymidine kinase confirming that these compound enters the active site of the enzyme and acts as alternate substrate | Entamoeba histolytica | ? | - |
? | |
ATP + beta-5-ethyl-2'-deoxyuridine | beta-5-ethyl-2'-deoxyuridine is efficiently phosphorylated by thymidine kinase confirming that these compound enters the active site of the enzyme and acts as alternate substrate | Entamoeba histolytica | ? | - |
? | |
ATP + thymidine | ATP acts as specific phosphate donor | Entamoeba histolytica | ADP + thymidine 5'-phosphate | - |
? | |
additional information | beta-L-thymidine is poorly phosphorylated by the enzyme | Entamoeba histolytica | ? | - |
? | |
additional information | does not use UTP, GTP, and TTP as cosubstrates | Entamoeba histolytica | ? | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
exhibits best activity at pH 8.0 at an ATP concentration range between 0.2 and 4.0 mM | Entamoeba histolytica |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Entamoeba histolytica |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.0006 | - |
at 37°C for 20 min in 30 mM potassium phosphate, pH 8.0, 1.2 mM MgCl2 | Entamoeba histolytica | 5-trifluoromethyl-2-deoxyuridine | |
0.001 | - |
at 37°C for 20 min in 30 mM potassium phosphate, pH 8.0, 1.2 mM MgCl2 | Entamoeba histolytica | 5-iodo-2'-deoxyuridine | |
0.0025 | - |
at 37°C for 20 min in 30 mM potassium phosphate, pH 8.0, 1.2 mM MgCl2 | Entamoeba histolytica | beta-D-5-ethyl-2'-deoxyuridine | |
0.005 | - |
at 37°C for 20 min in 30 mM potassium phosphate, pH 8.0, 1.2 mM MgCl2 | Entamoeba histolytica | beta-L-thymidine | |
0.01 | - |
at 37°C for 20 min in 30 mM potassium phosphate, pH 8.0, 1.2 mM MgCl2 | Entamoeba histolytica | penciclovir | |
0.015 | - |
at 37°C for 20 min in 30 mM potassium phosphate, pH 8.0, 1.2 mM MgCl2 | Entamoeba histolytica | 6-methyluridine | |
0.017 | - |
- |
Entamoeba histolytica | alpha-D-5-ethyl-2'-deoxyuridine | |
0.018 | - |
at 37°C for 20 min in 30 mM potassium phosphate, pH 8.0, 1.2 mM MgCl2 | Entamoeba histolytica | 3'-ethyl-5-methyl-2'-deoxyuridine | |
0.035 | - |
at 37°C for 20 min in 30 mM potassium phosphate, pH 8.0, 1.2 mM MgCl2 | Entamoeba histolytica | alpha-L-thymidine | |
0.0444 | - |
at 37°C for 20 min in 30 mM potassium phosphate, pH 8.0, 1.2 mM MgCl2 | Entamoeba histolytica | 5-propyl-2'-deoxyuridine |