Cloned (Comment) | Organism |
---|---|
expression of the His-tagged, thrombin-cleavable full length or truncated enzyme in Escherichia coli strain BL21 C41 (DE3) | Herpes simplex virus type 4 |
Crystallization (Comment) | Organism |
---|---|
full length and truncated enzyme in complex with thymidine and ADP, or with thymidine, sulfate, and a bisubstrate analogues, or with TP4A, or with TP5A, hanging drop vapour diffusion method, reservoir solution containing 0.1 M MES, pH 6.5, 1.2 M ammonium sulfate, and 3 or 5% dioxane, dependent on the complex ligand, mixed with enzyme solution containing 5.5 mg/ml protein, 1.0 mM TMP, 2.0 mM ADP, 25 mM HEPES, pH 7.0, 5 mM MgSO4, 5 mM DTT, 100 mM KCl, and 5% glycerol, at equal volumes of 0.001 ml on a glass cover slide, X-ray diffraction structure determination and analysis at 2.0-3.0 A resolution, molecular replacement method | Herpes simplex virus type 4 |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
36500 | - |
x * 38800, about, recombinant full length enzyme, amino acid sequence calculation, x * 36500, about, recombinant truncated enzyme, amino acid sequence calculation | Herpes simplex virus type 4 |
38800 | - |
x * 38800, about, recombinant full length enzyme, amino acid sequence calculation, x * 36500, about, recombinant truncated enzyme, amino acid sequence calculation | Herpes simplex virus type 4 |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + thymidine | Herpes simplex virus type 4 | - |
ADP + thymidine 5'-phosphate | - |
r | |
ATP + thymidine 5'-phosphate | Herpes simplex virus type 4 | - |
ADP + thymidine diphosphate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Herpes simplex virus type 4 | - |
type 4 | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged, thrombin-cleavable full length or truncated enzyme from Escherichia coli strain BL21 C41 (DE3) by nickel affinity chromatography, cleavage by thrombin | Herpes simplex virus type 4 |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
ATP + thymidine = ADP + dTMP | substrate binding site structures, structural basis for substrate specificity and activity | Herpes simplex virus type 4 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + thymidine | - |
Herpes simplex virus type 4 | ADP + thymidine 5'-phosphate | - |
r | |
ATP + thymidine 5'-phosphate | - |
Herpes simplex virus type 4 | ADP + thymidine diphosphate | - |
r | |
additional information | wide substrate specificity phosphorylating pyrimidine nucleosides, nucleoside monophophates, and several nucleoside analogues | Herpes simplex virus type 4 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 38800, about, recombinant full length enzyme, amino acid sequence calculation, x * 36500, about, recombinant truncated enzyme, amino acid sequence calculation | Herpes simplex virus type 4 |
Synonyms | Comment | Organism |
---|---|---|
EHV4-TK | - |
Herpes simplex virus type 4 |
thymidylate kinase | - |
Herpes simplex virus type 4 |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Herpes simplex virus type 4 |