Cloned (Comment) | Organism |
---|---|
expression of wild-type and Val106 mutants in an enzyme-deficient Escherichia coli strain as GST-fusion proteins | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
V106A | site-directed mutagenesis, mutant is similar to the wild-type enzyme in size, conformation and polarity, unaltered activity and oligomerization pattern | Homo sapiens |
V106G | site-directed mutagenesis, mutant differs in size, conformation and polarity from the wild-type enzyme, reduced activity, permanent tetrameric form irrespective of the presence of ATP | Homo sapiens |
V106H | site-directed mutagenesis, mutant differs in size, conformation and polarity from the wild-type enzyme, reduced activity, permanent tetrameric form irrespective of the presence of ATP | Homo sapiens |
V106I | site-directed mutagenesis, mutant is similar to the wild-type enzyme in size, conformation and polarity, unaltered activity and oligomerization pattern | Homo sapiens |
V106K | site-directed mutagenesis, mutant differs in size, conformation and polarity from the wild-type enzyme, reduced activity, permanent tetrameric form irrespective of the presence of ATP | Homo sapiens |
V106L | site-directed mutagenesis, mutant differs in size, conformation and polarity from the wild-type enzyme, reduced activity, permanent tetrameric form irrespective of the presence of ATP | Homo sapiens |
V106M | site-directed mutagenesis, mutant differs in size, conformation and polarity from the wild-type enzyme, reduced activity, permanent tetrameric form irrespective of the presence of ATP | Homo sapiens |
V106Q | site-directed mutagenesis, mutant differs in size, conformation and polarity from the wild-type enzyme, reduced activity, permanent tetrameric form irrespective of the presence of ATP | Homo sapiens |
V106T | site-directed mutagenesis, mutant is similar to the wild-type enzyme in size, conformation and polarity, unaltered activity and oligomerization pattern | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics of wild-type and mutant enzymes in absence or presence of ATP, overview | Homo sapiens | |
0.0003 | - |
thymidine | pH 7.5, recombinant mutant V106H, in absence of ATP | Homo sapiens | |
0.0003 | - |
thymidine | pH 7.5, recombinant mutants V106A and V106G, in presence of ATP | Homo sapiens | |
0.0004 | - |
thymidine | pH 7.5, recombinant mutants V106L and V106G, in absence of ATP | Homo sapiens | |
0.0004 | - |
thymidine | pH 7.5, recombinant mutants V106L, V106H and V106T, in presence of ATP | Homo sapiens | |
0.0006 | - |
thymidine | pH 7.5, recombinant mutant V106M, in absence or presence of ATP | Homo sapiens | |
0.0006 | - |
thymidine | pH 7.5, recombinant wild-type enzyme, in presence of ATP | Homo sapiens | |
0.0007 | - |
thymidine | pH 7.5, recombinant mutant V106K, in absence of ATP | Homo sapiens | |
0.0008 | - |
thymidine | pH 7.5, recombinant mutant V106Q, in absence or presence of ATP | Homo sapiens | |
0.0009 | - |
thymidine | pH 7.5, recombinant mutant V106I, in presence of ATP | Homo sapiens | |
0.0012 | - |
thymidine | pH 7.5, recombinant mutant V106K, in presence of ATP | Homo sapiens | |
0.0127 | - |
thymidine | pH 7.5, recombinant mutant V106A, in absence of ATP | Homo sapiens | |
0.0271 | - |
thymidine | pH 7.5, recombinant wild-type enzyme, in absence of ATP | Homo sapiens | |
0.0294 | - |
thymidine | pH 7.5, recombinant mutant V106I, in absence of ATP | Homo sapiens | |
0.043 | - |
thymidine | pH 7.5, recombinant mutant V106T, in absence of ATP | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Homo sapiens | 5829 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
24000 | - |
x * 24000, about, SDS-PAGE | Homo sapiens |
50000 | - |
approximately, dimeric enzyme form | Homo sapiens |
100000 | - |
approximately, tetrameric enzyme form | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + thymidine | Homo sapiens | - |
ADP + thymidine 5'-phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant GST-tagged wild-type and mutant enzymes from Escherichia coli by GST affinity chromatography, cleaving of the tag | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
lymphocyte | - |
Homo sapiens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
activities of recombinant wild-type and mutant enzymes in dimeric or tetrameric form, i.e. in absence or presence of ATP, overview | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + thymidine | - |
Homo sapiens | ADP + thymidine 5'-phosphate | - |
? | |
ATP + thymidine | enzyme contains the putative thymidine-binding motif FQRK | Homo sapiens | ADP + thymidine 5'-phosphate | - |
? | |
additional information | Val106 has effects on structure-function relation of the enzyme | Homo sapiens | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 24000, about, SDS-PAGE | Homo sapiens |
More | oligomerization patterns of wild-type and mutant enzymes, binding of ATP induces reversible transition from a dimer with low catalytic activity to a tetramer with high catalytic activity, Val106 is involved | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
More | the enzyme belongs to the thymidine kinase 1, i.e. TK1, family of enzymes | Homo sapiens |
thymidine kinase 1 | - |
Homo sapiens |
TK1 | - |
Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | binding of ATP induces reversible transition from a dimer with low catalytic activiy to a tetramer with high catalytic activity | Homo sapiens |