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Literature summary for 2.7.1.21 extracted from

  • Tsukifuji-Nabeya, R.; Yusa, T.; Kuroiwa, N.; Kumazawa, T.; Tamiya, N.; Moriyama, Y.; Okamoto, S.; Yamaguchi, Y.; Fujimura, S.
    Characterization of the purified cytosolic thymidine kinase from murine ehrlich ascites tumor: interconversion of two different relative molecular weight forms (1996), Biochem. Mol. Biol. Int., 40, 379-388.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
ATP
-
Mus musculus
dithiothreitol
-
Mus musculus

General Stability

General Stability Organism
dithiothreitol, ATP and other nucleosides stabilize Mus musculus

Localization

Localization Comment Organism GeneOntology No. Textmining

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
26000
-
x * 26000, SDS-PAGE Mus musculus
50000
-
gel filtration, two enzyme forms of MW 50000 and 70000 Mus musculus
70000
-
gel filtration, two enzyme forms of MW 50000 and 70000 Mus musculus

Organism

Organism UniProt Comment Textmining
Mus musculus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
18000fold, 2 different forms Mus musculus

Source Tissue

Source Tissue Comment Organism Textmining
Ehrlich ascites carcinoma cell
-
Mus musculus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.6
-
-
Mus musculus

Subunits

Subunits Comment Organism
? x * 26000, SDS-PAGE Mus musculus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
37
-
20% loss of activity in the presence of bovine serum albumin, thymidine and dithiothreitol after 60 min, almost complete loss of activity witout bovine serum albumin, thymidine and dithiothreitol after 60 min Mus musculus