Cloned (Comment) | Organism |
---|---|
- |
Herpes simplex virus |
Protein Variants | Comment | Organism |
---|---|---|
Q125D | increased Km for thymidine | Herpes simplex virus |
Q125E | increased Km for thymidine | Herpes simplex virus |
Q125N | increased Km for thymidine and ganciclovir | Herpes simplex virus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0009 | - |
thymidine | pH 7.6, 37°C, wild-type | Herpes simplex virus | |
0.003 | - |
thymidine | pH 7.6, 37°C, mutant Q125N | Herpes simplex virus | |
0.02 | - |
thymidine | pH 7.6, 37°C, mutant Q125E | Herpes simplex virus | |
0.05 | - |
ganciclovir | pH 7.6, 37°C, mutant Q125E | Herpes simplex virus | |
0.069 | - |
ganciclovir | pH 7.6, 37°C, wild-type | Herpes simplex virus | |
0.473 | - |
ganciclovir | pH 7.6, 37°C, mutant Q125N | Herpes simplex virus | |
6 | - |
thymidine | pH 7.6, 37°C, mutant Q125D | Herpes simplex virus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Herpes simplex virus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Herpes simplex virus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + ganciclovir | - |
Herpes simplex virus | ? | - |
? | |
ATP + thymidine | - |
Herpes simplex virus | ADP + dTMP | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.003 | - |
thymidine | pH 7.6, 37°C, mutant Q125N | Herpes simplex virus | |
0.04 | - |
ganciclovir | pH 7.6, 37°C, mutant Q125N | Herpes simplex virus | |
0.06 | - |
thymidine | pH 7.6, 37°C, wild-type | Herpes simplex virus | |
0.08 | - |
ganciclovir | pH 7.6, 37°C, mutant Q125E | Herpes simplex virus | |
0.11 | - |
thymidine | pH 7.6, 37°C, mutant Q125D | Herpes simplex virus | |
0.13 | - |
thymidine | pH 7.6, 37°C, mutant Q125E | Herpes simplex virus | |
0.47 | - |
ganciclovir | pH 7.6, 37°C, wild-type | Herpes simplex virus |