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Literature summary for 2.7.1.21 extracted from

  • Hinds, T.A.; Compadre, C.; Hurlburt, B.K.; Drake, R.R.
    Conservative mutations of glutamine-125 in Herpes simplex virus type 1 thymidine kinase result in a Ganciclovir kinase with minimal deoxypyrimidine kinase activities (2000), Biochemistry, 39, 4105-4111.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
-
Herpes simplex virus

Protein Variants

Protein Variants Comment Organism
Q125D increased Km for thymidine Herpes simplex virus
Q125E increased Km for thymidine Herpes simplex virus
Q125N increased Km for thymidine and ganciclovir Herpes simplex virus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0009
-
thymidine pH 7.6, 37°C, wild-type Herpes simplex virus
0.003
-
thymidine pH 7.6, 37°C, mutant Q125N Herpes simplex virus
0.02
-
thymidine pH 7.6, 37°C, mutant Q125E Herpes simplex virus
0.05
-
ganciclovir pH 7.6, 37°C, mutant Q125E Herpes simplex virus
0.069
-
ganciclovir pH 7.6, 37°C, wild-type Herpes simplex virus
0.473
-
ganciclovir pH 7.6, 37°C, mutant Q125N Herpes simplex virus
6
-
thymidine pH 7.6, 37°C, mutant Q125D Herpes simplex virus

Organism

Organism UniProt Comment Textmining
Herpes simplex virus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Herpes simplex virus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + ganciclovir
-
Herpes simplex virus ?
-
?
ATP + thymidine
-
Herpes simplex virus ADP + dTMP
-
?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.003
-
thymidine pH 7.6, 37°C, mutant Q125N Herpes simplex virus
0.04
-
ganciclovir pH 7.6, 37°C, mutant Q125N Herpes simplex virus
0.06
-
thymidine pH 7.6, 37°C, wild-type Herpes simplex virus
0.08
-
ganciclovir pH 7.6, 37°C, mutant Q125E Herpes simplex virus
0.11
-
thymidine pH 7.6, 37°C, mutant Q125D Herpes simplex virus
0.13
-
thymidine pH 7.6, 37°C, mutant Q125E Herpes simplex virus
0.47
-
ganciclovir pH 7.6, 37°C, wild-type Herpes simplex virus