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Literature summary for 2.7.1.21 extracted from
- Purification and properties of deoxythymidine kinase induced by bacteriophage T4 infection (1977), J. Biochem., 82, 1347-1359.
General Stability
| General Stability | Organism |
|---|---|
| dTTP protects against thermal inactivation | Tequatrovirus T4 |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ATP | non-competitive | Tequatrovirus T4 |  |
| Ba2+ | 0.29 mM | Tequatrovirus T4 | |
| dTTP | inhibition dependent on pH | Tequatrovirus T4 | |
| Mg2+ | non-competitive | Tequatrovirus T4 | |
| Zn2+ | 1.1 mM and 2.9 mM | Tequatrovirus T4 | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | - |
Tequatrovirus T4 |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | can partially substitute for Mg2+ | Tequatrovirus T4 | |
| Co2+ | can partially substitute for Mg2+ | Tequatrovirus T4 | |
| Mg2+ | - |
Tequatrovirus T4 | |
| Mn2+ | can partially substitute for Mg2+ | Tequatrovirus T4 | |
| Ni2+ | can partially substitute for Mg2+ | Tequatrovirus T4 | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 28000 | - |
x * 28000, SDS-PAGE | Tequatrovirus T4 |
| 86000 | - |
gel filtration | Tequatrovirus T4 |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Tequatrovirus T4 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Escherichia coli infected with bacteriophage | Tequatrovirus T4 |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 11 | - |
- |
Tequatrovirus T4 |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + thymidine | ATP can be substituted by dATP | Tequatrovirus T4 | ADP + dTMP | - |
? | |
| additional information | no phosphorylation of deoxyuridine, 5-halogenated deoxyuridines | Tequatrovirus T4 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 28000, SDS-PAGE | Tequatrovirus T4 |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
quick inactivation above, dTTP protects against thermal inactivation | Tequatrovirus T4 |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.8 | - |
- |
Tequatrovirus T4 |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 8 | 60% of maximal activity at pH 5.0, 50% of maximal activity at pH 8.0 | Tequatrovirus T4 |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2.5 | - |
Mg2+ | pH 7, 37°C | Tequatrovirus T4 | |
| 3.8 | - |
ATP | pH 7, 37°C | Tequatrovirus T4 | |
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