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Literature summary for 2.7.1.21 extracted from

  • Okazaki, R.; Kornberg, A.
    Deoxythymidine kinase of Escherichia coli. I. Purification and some properties of the enzyme (1964), J. Biol. Chem., 239, 269-274.
    View publication on PubMed
Search for more literature for 2.7.1.21

Activating Compound

Activating Compound Comment Organism Structure
bovine serum albumin activation at 0.2 mg/ml Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ 2.9 mM, required Escherichia coli
Mn2+ can partially substitute for Mg2+ Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4
-
-
 Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 5-bromo-2'-deoxyuridine
-
 Escherichia coli ADP + 5-bromo-2'-deoxyuridine 5'-phosphate
-
 ?
ATP + 5-chloro-2'-deoxyuridine
-
 Escherichia coli ADP + 5-chloro-2'-deoxyuridine 5'-phosphate
-
 ?
ATP + 5-fluoro-2'-deoxyuridine
-
 Escherichia coli ADP + 5-fluoro-2'-deoxyuridine 5'-phosphate
-
 ?
ATP + 5-iodo-2'-deoxyuridine
-
 Escherichia coli ADP + 5-iodo-2'-deoxyuridine 5'-phosphate
-
 ?
ATP + thymidine ATP can be substituted by dGTP Escherichia coli ADP + dTMP
-
 ?
ATP + thymidine requirement for high levels of ATP Escherichia coli ADP + dTMP
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
 Escherichia coli