Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Trypanosoma cruzi |
expression in Escherichia coli | Leishmania major |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.35 | - |
ATP | - |
Leishmania major | |
0.36 | - |
ATP | - |
Trypanosoma cruzi | |
1 | - |
D-glucose | - |
Trypanosoma cruzi | |
2.5 | 5 | D-glucose | recombinant enzyme without His6 | Leishmania major | |
3.3 | - |
D-glucose | recombinant (His)6-tagged enzyme | Leishmania major |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
glycosome | - |
Trypanosoma cruzi | 20015 | - |
glycosome | - |
Leishmania major | 20015 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
43000 | - |
x * 43000, the enzyme may occur as a monomer or dimer, dependent on the protein concentration, SDS-PAGE | Trypanosoma cruzi |
46000 | - |
monomer, gel filtration | Trypanosoma cruzi |
46000 | - |
x * 46000, the enzyme may occur as a monomer or dimer, dependent on the protein concentration, SDS-PAGE | Leishmania major |
49000 | - |
monomer, gel filtration | Leishmania major |
86000 | - |
dimer, gel filtration | Trypanosoma cruzi |
86000 | - |
dimer, gel filtration | Leishmania major |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Leishmania major | Q4Q1I9 | - |
- |
Trypanosoma cruzi | Q4E4E1 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme | Trypanosoma cruzi |
recombinant enzyme | Leishmania major |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
epimastigote | - |
Trypanosoma cruzi | - |
promastigote | - |
Leishmania major | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + D-glucose | enzyme is only able to phosphorylate glucose | Trypanosoma cruzi | ADP + D-glucose 6-phosphate | - |
? | |
ATP + D-glucose | enzyme is only able to phosphorylate glucose | Leishmania major | ADP + D-glucose 6-phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 43000, the enzyme may occur as a monomer or dimer, dependent on the protein concentration, SDS-PAGE | Trypanosoma cruzi |
? | x * 46000, the enzyme may occur as a monomer or dimer, dependent on the protein concentration, SDS-PAGE | Leishmania major |
Synonyms | Comment | Organism |
---|---|---|
LmGlcK | - |
Leishmania major |
TcGlcK | - |
Trypanosoma cruzi |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
317 | - |
ATP | - |
Leishmania major | |
317 | - |
D-glucose | - |
Leishmania major | |
1492 | - |
ATP | - |
Trypanosoma cruzi | |
1492 | - |
D-glucose | - |
Trypanosoma cruzi |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | 9 | - |
Trypanosoma cruzi |
8.5 | 9 | - |
Leishmania major |