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Literature summary for 2.7.1.2 extracted from
- Thermostable continuous coupled assay for measuring glucose using glucokinase and glucose-6-phosphate dehydrogenase from the marine hyperthermophile Thermotoga maritima (2003), Anal. Biochem., 318, 196-203.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene TM 1469, expression in Escherichia coli strain BL21(DE3) | Thermotoga maritima |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | kinetics | Thermotoga maritima | |
| 0.038 | - |
D-glucose | pH 8.0, 85°C, recombinant enzyme | Thermotoga maritima |  |
| 0.189 | - |
ATP | pH 8.0, 85°C, recombinant enzyme | Thermotoga maritima | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | at 4.4 mM MgCl2 | Thermotoga maritima | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| additional information | - |
recombinant enzyme, native PAGE | Thermotoga maritima |
| 33500 | - |
2 * 33500, the recombinant enzyme probably is a dimer, SDS-PAGE | Thermotoga maritima |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + D-glucose | Thermotoga maritima | - |
ADP + D-glucose 6-phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thermotoga maritima | - |
gene TM 1469 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme from Escherichia coli strain BL21(DE3) by heat treatment and Ni2+ chelating affinity chromatography | Thermotoga maritima |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 166 | - |
purified recombinant enzyme | Thermotoga maritima |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + D-glucose | - |
Thermotoga maritima | ADP + D-glucose 6-phosphate | - |
? | |
| additional information | no activity with fructose, galactose, and mannose, development of a coupled assay method with glucose 6-phosphate dehydrogenase stable at pH 6.8-8.5 and 75-90°C, overview | Thermotoga maritima | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 33500, the recombinant enzyme probably is a dimer, SDS-PAGE | Thermotoga maritima |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| glk | - |
Thermotoga maritima |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 85 | - |
- |
Thermotoga maritima |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
development of an assay method stable at pH 6.8-8.5 and 75-90°C, overview | Thermotoga maritima |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 92.6 | - |
D-glucose | pH 8.0, 85°C, recombinant enzyme | Thermotoga maritima | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
sharp optimum | Thermotoga maritima |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| additional information | - |
development of an assay method stable at pH 6.8-8.5 and 75-90°C, overview | Thermotoga maritima |
| 7 | 8.5 | - |
Thermotoga maritima |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Thermotoga maritima | |
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Release 2023.1 (January 2023)
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