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Literature summary for 2.7.1.17 extracted from
- Genomic analysis of a xylose operon and characterization of novel xylose isomerase and xylulokinase from Bacillus coagulans NL01 (2016), Biotechnol. Lett., 38, 1331-1339.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Weizmannia coagulans |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| phosphate | activity of xylulokinase in 100 mM Tris/HCl decreases by 70% in 100 mM NaH2PO4/NaOH at pH 7 | Weizmannia coagulans |  |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | or Mn2+, Fe2+, may substitute for Mg2+ | Weizmannia coagulans | |
| Fe2+ | or Mn2+, Co2+, may substitute for Mg2+ | Weizmannia coagulans | |
| Mg2+ | or Co2+, Mn2+, Fe2+, required. Assay in presence of 10 mM | Weizmannia coagulans | |
| Mn2+ | or Co2+, Fe2+, may substitute for Mg2+ | Weizmannia coagulans | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 56000 | - |
- |
Weizmannia coagulans |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Weizmannia coagulans | - |
- |
- |
| Weizmannia coagulans NL01 | - |
- |
- |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1.62 | - |
pH 7.5, temperature not specified in the publication | Weizmannia coagulans |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + D-xylulose | - |
Weizmannia coagulans | ADP + D-xylulose 5-phosphate | - |
? | |
| ATP + D-xylulose | - |
Weizmannia coagulans NL01 | ADP + D-xylulose 5-phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 56000, calculated, x * 56000-58000, SDS-PAGE | Weizmannia coagulans |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| XylB | - |
Weizmannia coagulans |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 85 | - |
- |
Weizmannia coagulans |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Weizmannia coagulans |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 9 | - |
5 h, 75% residual activity | Weizmannia coagulans |
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