Activating Compound | Comment | Organism | Structure |
---|---|---|---|
EDTA | no activation | Scheffersomyces stipitis |
General Stability | Organism |
---|---|
EDTA and MgCl2 partially stabilize | Scheffersomyces stipitis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | weak | Scheffersomyces stipitis | |
KCl | weak | Scheffersomyces stipitis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.28 | - |
ATP | - |
Scheffersomyces stipitis | |
0.52 | - |
D-xylulose | - |
Scheffersomyces stipitis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Scheffersomyces stipitis | |
Mn2+ | 35% of the activation with Mg2+ | Scheffersomyces stipitis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
71000 | - |
2 * 71000, SDS-PAGE | Scheffersomyces stipitis |
120000 | 130000 | gel filtration | Scheffersomyces stipitis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + D-xylulose | Scheffersomyces stipitis | - |
? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Scheffersomyces stipitis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Scheffersomyces stipitis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
21.4 | - |
- |
Scheffersomyces stipitis |
Storage Stability | Organism |
---|---|
-20°C, 1 month stable and 20% of original activity retained after 8 months | Scheffersomyces stipitis |
4°C, inactivation within 24 h | Scheffersomyces stipitis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + D-xylulose | - |
Scheffersomyces stipitis | ? | - |
? | |
ATP + D-xylulose | specific for D-isomer | Scheffersomyces stipitis | ADP + D-xylulose 5-phosphate | - |
r | |
ATP + L-erythrulose | phosphorylation at 30% the rate of D-xylulose | Scheffersomyces stipitis | ADP + ? | - |
? | |
GTP + D-xylulose | no activity | Scheffersomyces stipitis | GDP + D-xylulose 5-phosphate | - |
? | |
UTP + D-xylulose | no activity | Scheffersomyces stipitis | UDP + D-xylulose 5-phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 71000, SDS-PAGE | Scheffersomyces stipitis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
- |
Scheffersomyces stipitis |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
10 | 40 | steady increase up to 30°C, rapid loss above, with 60% of maximal activity at 40°C | Scheffersomyces stipitis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | 7.8 | - |
Scheffersomyces stipitis |