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Literature summary for 2.7.1.17 extracted from

  • Waites, M.J.; Flanagan, T.
    Purification and characterization of D-xylulokinase from the pentose-fermenting yeast Pichia stipitis NCYC 1541 (1992), Enzyme Microb. Technol., 14, 975-979.
No PubMed abstract available

Activating Compound

Activating Compound Comment Organism Structure
EDTA no activation Scheffersomyces stipitis

General Stability

General Stability Organism
EDTA and MgCl2 partially stabilize Scheffersomyces stipitis

Inhibitors

Inhibitors Comment Organism Structure
EDTA weak Scheffersomyces stipitis
KCl weak Scheffersomyces stipitis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.28
-
ATP
-
Scheffersomyces stipitis
0.52
-
D-xylulose
-
Scheffersomyces stipitis

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
Scheffersomyces stipitis
Mn2+ 35% of the activation with Mg2+ Scheffersomyces stipitis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
71000
-
2 * 71000, SDS-PAGE Scheffersomyces stipitis
120000 130000 gel filtration Scheffersomyces stipitis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + D-xylulose Scheffersomyces stipitis
-
?
-
?

Organism

Organism UniProt Comment Textmining
Scheffersomyces stipitis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Scheffersomyces stipitis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
21.4
-
-
Scheffersomyces stipitis

Storage Stability

Storage Stability Organism
-20°C, 1 month stable and 20% of original activity retained after 8 months Scheffersomyces stipitis
4°C, inactivation within 24 h Scheffersomyces stipitis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + D-xylulose
-
Scheffersomyces stipitis ?
-
?
ATP + D-xylulose specific for D-isomer Scheffersomyces stipitis ADP + D-xylulose 5-phosphate
-
r
ATP + L-erythrulose phosphorylation at 30% the rate of D-xylulose Scheffersomyces stipitis ADP + ?
-
?
GTP + D-xylulose no activity Scheffersomyces stipitis GDP + D-xylulose 5-phosphate
-
?
UTP + D-xylulose no activity Scheffersomyces stipitis UDP + D-xylulose 5-phosphate
-
?

Subunits

Subunits Comment Organism
dimer 2 * 71000, SDS-PAGE Scheffersomyces stipitis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
Scheffersomyces stipitis

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
10 40 steady increase up to 30°C, rapid loss above, with 60% of maximal activity at 40°C Scheffersomyces stipitis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2 7.8
-
Scheffersomyces stipitis