Cloned (Comment) | Organism |
---|---|
functional recombinant expression of wild-type and K802R mutant enzymes in Saccharomyces cerevisiae, the transcriptional profile of PI3K-expressing cells is consistent with sustained CWI activation, Pkc1 localizes to endosomes in PI3K-expressing cells, PI3K expression relocates Pkc1 to endosomal compartments. PI3K effects in yeast signaling and trafficking are reversible by a competitive inhibitor in a dose-dependent manner | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
K802R | mutation in subunit p110alpha, inactive mutant | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
plasma membrane | - |
Homo sapiens | 5886 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate | Homo sapiens | - |
ADP + 1-phosphatidyl-1D-myo-inositol 3,4,5-trisphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P42336 AND P27986 | isozyme PI3Kalpha | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate | - |
Homo sapiens | ADP + 1-phosphatidyl-1D-myo-inositol 3,4,5-trisphosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
class I phosphoinositide 3-kinase | - |
Homo sapiens |
PI3Kalpha | - |
Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Homo sapiens |
General Information | Comment | Organism |
---|---|---|
physiological function | phosphatidylinositol 4,5-bisphosphate is essential for recognition of the plasma membrane inner leaf by protein complexes. Elimination of phosphatidylinositol 4,5-bisphosphate by its conversion into 1-phosphatidyl-1D-myo-inositol 3,4,5-trisphosphate, a lipid naturally missing in this yeast. The loss of 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate leads to loss of actin function and endocytosis defects, causing a blockage in polarized secretion. Also, the cell wall integrity mitogen-activated protein kinase pathway is activated, triggering a typical transcriptional response. In the absence of 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate at the plasma membrane, the Pkc1 protein kinase upstream the cell wall integrity mitogen-activated protein kinase pathway module localizes to post-Golgi endosomes marked by SNARE Snc1 and Rab GTPases Ypt31 and Ypt32. Phosphatidylinositol 4,5-bisphosphate depletion activates the CWI pathway and generates a transcriptional profile reminiscent of that induced by cellwall aggressions. PI3K effects in yeast signaling and trafficking are reversible by a competitive inhibitor in a dose-dependent manner | Homo sapiens |