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Literature summary for 2.7.1.153 extracted from

  • Fernandez-Acero, T.; Rodriguez-Escudero, I.; Molina, M.; Cid, V.J.
    The yeast cell wall integrity pathway signals from recycling endosomes upon elimination of phosphatidylinositol (4,5)-bisphosphate by mammalian phosphatidylinositol 3-kinase (2015), Cell. Signal., 27, 2272-2284.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
functional recombinant expression of wild-type and K802R mutant enzymes in Saccharomyces cerevisiae, the transcriptional profile of PI3K-expressing cells is consistent with sustained CWI activation, Pkc1 localizes to endosomes in PI3K-expressing cells, PI3K expression relocates Pkc1 to endosomal compartments. PI3K effects in yeast signaling and trafficking are reversible by a competitive inhibitor in a dose-dependent manner Homo sapiens

Protein Variants

Protein Variants Comment Organism
K802R mutation in subunit p110alpha, inactive mutant Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
plasma membrane
-
Homo sapiens 5886
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate Homo sapiens
-
ADP + 1-phosphatidyl-1D-myo-inositol 3,4,5-trisphosphate
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens P42336 AND P27986 isozyme PI3Kalpha
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate
-
Homo sapiens ADP + 1-phosphatidyl-1D-myo-inositol 3,4,5-trisphosphate
-
?

Synonyms

Synonyms Comment Organism
class I phosphoinositide 3-kinase
-
Homo sapiens
PI3Kalpha
-
Homo sapiens

Cofactor

Cofactor Comment Organism Structure
ATP
-
Homo sapiens

General Information

General Information Comment Organism
physiological function phosphatidylinositol 4,5-bisphosphate is essential for recognition of the plasma membrane inner leaf by protein complexes. Elimination of phosphatidylinositol 4,5-bisphosphate by its conversion into 1-phosphatidyl-1D-myo-inositol 3,4,5-trisphosphate, a lipid naturally missing in this yeast. The loss of 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate leads to loss of actin function and endocytosis defects, causing a blockage in polarized secretion. Also, the cell wall integrity mitogen-activated protein kinase pathway is activated, triggering a typical transcriptional response. In the absence of 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate at the plasma membrane, the Pkc1 protein kinase upstream the cell wall integrity mitogen-activated protein kinase pathway module localizes to post-Golgi endosomes marked by SNARE Snc1 and Rab GTPases Ypt31 and Ypt32. Phosphatidylinositol 4,5-bisphosphate depletion activates the CWI pathway and generates a transcriptional profile reminiscent of that induced by cellwall aggressions. PI3K effects in yeast signaling and trafficking are reversible by a competitive inhibitor in a dose-dependent manner Homo sapiens