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Literature summary for 2.7.1.149 extracted from

  • Clarke, J.H.; Emson, P.C.; Irvine, R.F.
    Localization of Phosphatidylinositol phosphate Kinase II gamma in kidney to a membrane trafficking compartment within specialized cells of the nephron (2008), Am. J. Physiol. Renal Physiol., 295, F1422-1430.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
PIP5K2 genes are amplified from a whole human brain marathon-ready cDNA library using gene-specific primers. Recombinant protein is expressed from Escherichia coli harbouring PIP5K2s in pET-32a. PCR products are cloned, via incorporated HindIII and BamHI restriction sites, into appropriate plasmid vectors. HeLa, HEK 293, COS-7 and NRK cells are transiently transfected with plasmid constructs. Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
Golgi apparatus partial Golgi localization, primarily the matrix of the cis-Golgi Mus musculus 5794
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Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
47000
-
predicted Homo sapiens
47000
-
predicted and confirmed by western blotting, represents the phosphorylated form of mature PIP4Kgamma Mus musculus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + 1-phosphatidyl-1D-myo-inositol 5-phosphate Mus musculus
-
ADP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate
-
ir
ATP + 1-phosphatidyl-1D-myo-inositol 5-phosphate Homo sapiens
-
ADP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate
-
ir

Organism

Organism UniProt Comment Textmining
Homo sapiens Q8TBX8
-
-
Mus musculus Q91XU3
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
phosphoprotein
-
Mus musculus

Purification (Commentary)

Purification (Comment) Organism
By using TALON metal affinity resin. Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
brain high mRNA expression Mus musculus
-
epithelial cell restricted expression in the thick ascending limb of the loop of Henle and in the intercalated cells of the collecting duct Mus musculus
-
heart high mRNA expression Mus musculus
-
heart PIP4Kgamma in heart run predominantly as a 40 kDa band, suggesting that processing of this isoform occurs. Mus musculus
-
HEK-293 cell
-
Homo sapiens
-
HeLa cell
-
Homo sapiens
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kidney cortex and outer medulla, regulation of vesicular transport in specialized kidney epithelial cells Mus musculus
-
ovary
-
Mus musculus
-
testis high mRNA expression Mus musculus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
Bacterially-expressed recombinant PIP4Kgamma is completely inactive, but does have the ability to associate with active PIP4Kalpha in vitro. Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 1-phosphatidyl-1D-myo-inositol 5-phosphate
-
Mus musculus ADP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate
-
ir
ATP + 1-phosphatidyl-1D-myo-inositol 5-phosphate
-
Homo sapiens ADP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate
-
ir

Synonyms

Synonyms Comment Organism
1-phosphatidylinositol-5-phosphate 4-kinase
-
Mus musculus
1-phosphatidylinositol-5-phosphate 4-kinase
-
Homo sapiens
phosphatidylinositol phosphate kinase II gamma
-
Mus musculus
phosphatidylinositol phosphate kinase II gamma
-
Homo sapiens
PIP4K
-
Mus musculus
PIP4K
-
Homo sapiens
PIP4Kgamma isoform Mus musculus
PIP4Kgamma isoform Homo sapiens
PtdIns5P 4-kinase
-
Mus musculus
PtdIns5P 4-kinase
-
Homo sapiens

Cofactor

Cofactor Comment Organism Structure
ATP
-
Mus musculus