Activating Compound | Comment | Organism | Structure |
---|---|---|---|
light | Selective increase in the transcript abundance of GbCMK2 in the radicles by induction of light. | Ginkgo biloba | |
methyl jasmonate | Selective increase in the transcript abundance of GbCMK2 in adicles. | Ginkgo biloba |
Cloned (Comment) | Organism |
---|---|
The two CMK sequences encoding the mature CMK proteins are amplified with their respective primer pairs and cloned to the pMW118 vector to derive the pMW-CMK1 and the pMW-CMK2 plasmids. NMW29, an Escherichia coli ychB mutant harboring the pTMV20KM plasmid. The strain is transformed with the pMW-CMK1 and the pMW-CMK2 plasmids and selected on the LB medium without mevalonate. | Ginkgo biloba |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast | isoenzyme contains chloroplast transit peptide sequence | Ginkgo biloba | 9507 | - |
cytosol | - |
Ginkgo biloba | 5829 | - |
nucleus | - |
Ginkgo biloba | 5634 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
39200 | - |
theoretical value, predicted | Ginkgo biloba |
39900 | - |
theoretical value, predicted | Ginkgo biloba |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol + ATP | Ginkgo biloba | - |
2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol + ADP | - |
ir |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Ginkgo biloba | A6XAA8 | - |
- |
Ginkgo biloba | A6XAA9 | - |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
ATP + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol = ADP + 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol | catalyzes the fourth step of 2-C-methyl-D-erythritol 4-phosphate pathway | Ginkgo biloba |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cotyledon | 5 embryos are pooled to prepare a RNA sample, used for complementation assay | Ginkgo biloba | - |
radicle | 5 embryos are pooled to prepare a RNA sample, used for complementation assay | Ginkgo biloba | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol + ATP | - |
Ginkgo biloba | 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol + ADP | - |
ir |
Synonyms | Comment | Organism |
---|---|---|
4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase | - |
Ginkgo biloba |
CDP-ME kinase | - |
Ginkgo biloba |
CMK | - |
Ginkgo biloba |
GbCMK1 | Isoenzyme with 433 amino acids. The identity between GbCMK2 and GbCMK1 is 62.7%. | Ginkgo biloba |
GbCMK2 | isoenzyme with 428 amino acids. The identity between GbCMK2 and GbCMK1 is 62.7%. | Ginkgo biloba |
YchB | - |
Ginkgo biloba |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Ginkgo biloba |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Ginkgo biloba | theoretical value, predicted | - |
5.1 |
Ginkgo biloba | theoretical value, predicted | - |
5.2 |