Literature summary for 2.7.1.137 extracted from

Gray, A.; Olsson, H.; Batty, I.H.; Priganica, L.; Peter Downes, C.
Nonradioactive methods for the assay of phosphoinositide 3-kinases and phosphoinositide phosphatases and selective detection of signaling lipids in cell and tissue extracts (2003), Anal. Biochem., 313, 234-245.

Search for more literature for 2.7.1.137

Activating Compound

Activating Compound	Comment	Organism	Structure
additional information	enzyme induction in cell culture by platelet-derived growth factor PGDF and starvation via the G-protein-coupled fMLP receptor	Mus musculus

Cloned(Commentary)

Cloned (Comment)	Organism
expression of the GRP1 domain, comprising residues 263-380, in Escherichia coli	Mus musculus

Inhibitors

Inhibitors	Comment	Organism	Structure
EDTA	-	Mus musculus
LY294002	specific inhibition	Mus musculus
staurosporine	-	Mus musculus
Wortmannin	specific irreversible inhibition, binds covalently to the active site, mixed type inhibition, IC50 is 12 nM	Mus musculus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.035	-	ATP	pH 7.4, recombinant enzyme	Mus musculus
0.05	-	1,2-dioctanoylphosphatidylinositol 4,5-diphosphate	pH 7.4, recombinant enzyme	Mus musculus

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	dependent on, optimal at 5 mM	Mus musculus

Organism

Organism	UniProt	Comment	Textmining
Mus musculus	-	isozyme PI 3-kinase gamma	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant GRP1 domain, comprising residues 263-380, from Escherichia coli by glutathione affinity chromatography	Mus musculus

Source Tissue

Source Tissue	Comment	Organism	Textmining
brain	-	Mus musculus	-
NIH-3T3 cell	-	Mus musculus	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
additional information	-	assay method development and optimization, phosphoinositide quantification via labeled and/or tagged ligands binding to the pleckstrin homology PH domain of the recombinant enzyme in competition with phosphoinositides, overview	Mus musculus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + 1,2-dibutanoylphosphatidylinositol 4,5-diphosphate	-	Mus musculus	ADP + 1,2-dibutanoylphosphatidylinositol 3,4,5-trisphosphate	-	?
ATP + 1,2-dioctanoylphosphatidylinositol 4,5-diphosphate	-	Mus musculus	ADP + 1,2-dioctanoylphosphatidylinositol 3,4,5-trisphosphate	-	?
additional information	phosphoinositide quantification via labeled and/or tagged ligands binding to the pleckstrin homology PH domain of the enzyme in competition with phosphoinositides, overview	Mus musculus	?	-	?

Synonyms

Synonyms	Comment	Organism
phosphoinositide 3-kinase	-	Mus musculus
PI 3-kinase	-	Mus musculus

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.4	-	assay at	Mus musculus

Cofactor

Cofactor	Comment	Organism	Structure
ATP	-	Mus musculus

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.0038	-	staurosporine	pH 7.4, recombinant enzyme	Mus musculus
0.0056	-	LY294002	pH 7.4, recombinant enzyme	Mus musculus

IC50 Value

IC50 Value	IC50 Value Maximum	Comment	Organism	Inhibitor	Structure
0.000012	-	specific irreversible inhibition, binds covalently to the active site, mixed type inhibition, IC50 is 12 nM	Mus musculus	Wortmannin

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Release 2023.1 (January 2023)