Cloned (Comment) | Organism |
---|---|
transient expression of isozyme B fragment 108-170 fused to EGFP in NRK 52E and PC12 cells co-localizes with F-actin, overexpression of isozyme B fused to EGFP in Escherichia coli, the enzyme co-localizes in with the cytoskeleton as well as with the endoplasmic reticulum and the plasma membrane, overexpression of inactive isozyme B lacking the F-actin target sequence at the noncatalytical N-terminus | Homo sapiens |
transient expression of isozyme B in NRK 52E and PC12 cells, DNA and amino acid sequence determination and analysis, expression of isoform B comprising residues 555-934 as Strep-Tactin-tagged enzyme in Escherichia coli strain XL1-Blue, expression of isoform B comprising residues 108-170 as GST-fusion protein in Escherichia coli strain BL21(DE3) | Rattus norvegicus |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of a mutant isozyme B by helix-breaking mutations in the actin binding domain and deletion of the actin binding domain in full-length isozyme B resulting in loss of actin binding ability | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoskeleton | the enzyme binds to F-actin in the cellular actin cytoskeleton with its actin binding domain at the N-terminus | Homo sapiens | 5856 | - |
cytoskeleton | the enzyme binds to F-actin in the cellular actin cytoskeleton with its actin binding domain, within residues 108-170, at the N-terminus | Rattus norvegicus | 5856 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
isoform B | - |
Rattus norvegicus | P42335 | isoform B; isoform B | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
additional information | the enzyme is cleaved at the Asp-Pro clusters, residues 610, 617, and 765, at low pH and different temperatures producing 7 enzyme fragments of 17.4-82.3 kDa of the full-length enzyme of about 100 kDa, overview | Rattus norvegicus |
Purification (Comment) | Organism |
---|---|
recombinant GST-fusion isoform B comprising residues 108-170 by glutathione affinity chromatography, recombinant truncated Strep-tagged isozyme B by affinity chromatography | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | - |
Rattus norvegicus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 1D-myo-inositol 1,4,5-trisphosphate | - |
Homo sapiens | ADP + 1D-myo-inositol 1,3,4,5-tetrakisphosphate | - |
? | |
ATP + 1D-myo-inositol 1,4,5-trisphosphate | - |
Rattus norvegicus | ADP + 1D-myo-inositol 1,3,4,5-tetrakisphosphate | - |
? | |
additional information | the enzyme binds to F-actin in the cellular actin cytoskeleton with its F-actin domain at the N-terminus, the C-terminal fragment cannot bind to F-actin | Homo sapiens | ? | - |
? | |
additional information | the enzyme binds to F-actin in the cellular actin cytoskeleton with its F-actin domain at the N-terminus, the C-terminal fragment cannot bind to F-actin | Rattus norvegicus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Ins(1,4,5)P3 3-kinase isoform B | - |
Homo sapiens |
Ins(1,4,5)P3 3-kinase isoform B | - |
Rattus norvegicus |
IP3K-B | - |
Homo sapiens |
IP3K-B | - |
Rattus norvegicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Homo sapiens | |
ATP | - |
Rattus norvegicus |