Inhibitors | Comment | Organism | Structure |
---|---|---|---|
citrate | - |
Columba sp. | |
citrate | - |
Gallus gallus | |
additional information | phosphorylation by cAMP-dependent protein kinase causes inactivation of liver enzyme | Columba sp. | |
additional information | phosphorylation by cAMP-dependent protein kinase causes inactivation of liver enzyme | Gallus gallus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0072 | - |
MgATP2- | pH 7.1, 30°C, phosphorylated enzyme | Columba sp. | |
0.0095 | 0.012 | MgATP2- | pH 7.1, 30°C, native pigeon enzyme | Columba sp. | |
0.05 | - |
beta-D-fructose 6-phosphate | pH 7.1, 30°C, MgATP2-, phosphorylated chicken enzyme | Gallus gallus | |
0.29 | - |
MgATP2- | pH 7.1, 30°C, native chicken enzyme | Gallus gallus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
phosphate | - |
Gallus gallus | |
phosphate | - |
Columba sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
53000 | - |
2 * 53000, SDS-PAGE, with a minor constituent of MW 54000 | Columba sp. |
54000 | - |
2 * 54000, SDS-PAGE | Gallus gallus |
110000 | - |
gel filtration | Gallus gallus |
110000 | - |
gel filtration | Columba sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Columba sp. | - |
- |
- |
Gallus gallus | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
phosphoprotein | phosphorylation by cAMP-dependent protein kinase causes inactivation of liver enzyme | Gallus gallus |
phosphoprotein | phosphorylation by cAMP-dependent protein kinase causes inactivation of liver enzyme | Columba sp. |
Purification (Comment) | Organism |
---|---|
- |
Gallus gallus |
- |
Columba sp. |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Gallus gallus | - |
muscle | - |
Columba sp. | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.11 | - |
- |
Columba sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + beta-D-fructose 6-phosphate | - |
Gallus gallus | ADP + beta-D-fructose 2,6-bisphosphate | - |
r | |
ATP + beta-D-fructose 6-phosphate | - |
Columba sp. | ADP + beta-D-fructose 2,6-bisphosphate | - |
r | |
MgATP2- + beta-D-fructose 6-phosphate | - |
Gallus gallus | ? | - |
? | |
MgATP2- + beta-D-fructose 6-phosphate | - |
Columba sp. | ? | - |
? | |
additional information | also catalyses the degradation of fructose 2,6-bisphosphate (EC 3.1.3.46) | Gallus gallus | ? | - |
? | |
additional information | also catalyses the degradation of fructose 2,6-bisphosphate (EC 3.1.3.46) | Columba sp. | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 54000, SDS-PAGE | Gallus gallus |
dimer | 2 * 53000, SDS-PAGE, with a minor constituent of MW 54000 | Columba sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Gallus gallus |
30 | - |
assay at | Columba sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
10 | - |
- |
Gallus gallus |
10 | - |
- |
Columba sp. |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 10 | about 80% of maximal activity at pH 6.0 and 9.5 | Gallus gallus |
7.5 | 10 | linear increase up to pH 10 from 10% of maximal activity at pH 7.5 | Columba sp. |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.083 | - |
citrate | pH 7.1, 30°C, native enzyme | Columba sp. | |
0.084 | - |
citrate | pH 7.1, 30°C, phosphorylated enzyme | Columba sp. | |
0.13 | - |
citrate | pH 7.1, 30°C, phosphorylated enzyme | Gallus gallus | |
0.49 | - |
citrate | pH 7.1, 30°C, native enzyme | Gallus gallus |